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Statements

Subject Item
n2:RIV%2F61989592%3A15310%2F13%3A33147897%21RIV14-MSM-15310___
rdf:type
n5:Vysledek skos:Concept
dcterms:description
Here we introduce the software BIOSPEAN, which is applicable for processing of peptide/protein profiles obtained from MALDI intact cell/spore mass spectrometry. It has been developed as a web application using common technology. The BIOSPEAN automatically finds peaks in a mass spectrum. The peak detection principle involves a local scanning of intensity values around individual m/z positions. To cope with the level of noise, a threshold signal-to-noise ratio is adjusted for filtering relevant results. Based on the detected peak pattern, a similarity search in a custom spectral database can subsequently be performed for sample identification. When a spectrum is analyzed by comparison with a database entry, a percentage score value is calculated from the number of identical peak positions found (they are assigned with an adjustable mass tolerance) divided by the number of all detected peaks in the analyzed spectrum. Each two spectra may also be compared in the opposite way and both score values averaged. The database search results are finally sorted in a table. Here we introduce the software BIOSPEAN, which is applicable for processing of peptide/protein profiles obtained from MALDI intact cell/spore mass spectrometry. It has been developed as a web application using common technology. The BIOSPEAN automatically finds peaks in a mass spectrum. The peak detection principle involves a local scanning of intensity values around individual m/z positions. To cope with the level of noise, a threshold signal-to-noise ratio is adjusted for filtering relevant results. Based on the detected peak pattern, a similarity search in a custom spectral database can subsequently be performed for sample identification. When a spectrum is analyzed by comparison with a database entry, a percentage score value is calculated from the number of identical peak positions found (they are assigned with an adjustable mass tolerance) divided by the number of all detected peaks in the analyzed spectrum. Each two spectra may also be compared in the opposite way and both score values averaged. The database search results are finally sorted in a table.
dcterms:title
BIOSPEAN: A freeware tool for processing spectra from MALDI intact cell/spore mass spectrometry BIOSPEAN: A freeware tool for processing spectra from MALDI intact cell/spore mass spectrometry
skos:prefLabel
BIOSPEAN: A freeware tool for processing spectra from MALDI intact cell/spore mass spectrometry BIOSPEAN: A freeware tool for processing spectra from MALDI intact cell/spore mass spectrometry
skos:notation
RIV/61989592:15310/13:33147897!RIV14-MSM-15310___
n5:predkladatel
n6:orjk%3A15310
n3:aktivita
n19:P
n3:aktivity
P(7AMB12AT018), P(ED0007/01/01)
n3:cisloPeriodika
12
n3:dodaniDat
n7:2014
n3:domaciTvurceVysledku
n11:9708790 n11:5464900
n3:druhVysledku
n20:J
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n8:predkladatel
n3:idSjednocenehoVysledku
63556
n3:idVysledku
RIV/61989592:15310/13:33147897
n3:jazykVysledku
n9:eng
n3:klicovaSlova
Comparison, Database search, Mass spectrometry, Microorganisms, Peak, Software
n3:klicoveSlovo
n12:Software n12:Microorganisms n12:Database%20search n12:Comparison n12:Mass%20spectrometry n12:Peak
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[9167106C6656]
n3:nazevZdroje
Journal of Proteomics and Bioinformatics
n3:obor
n16:CE
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
2
n3:projekt
n4:7AMB12AT018 n4:ED0007%2F01%2F01
n3:rokUplatneniVysledku
n7:2013
n3:svazekPeriodika
6
n3:tvurceVysledku
Raus, Martin Šebela, Marek
s:issn
0974-276X
s:numberOfPages
5
n18:doi
10.4172/jpb.1000292
n15:organizacniJednotka
15310