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Statements

Subject Item
n2:RIV%2F61989592%3A15310%2F13%3A33147814%21RIV14-GA0-15310___
rdf:type
skos:Concept n10:Vysledek
rdfs:seeAlso
http://www.plantphysiol.org/content/161/3/1229.full.pdf+html
dcterms:description
In vitro shoot regeneration is implemented in basic plant research and commercial plant production, but for some plant species, it is still difficult to achieve by means of the currently available cytokinins and auxins. To identify novel compounds that promote shoot regeneration, we screened a library of 10,000 small molecules. The bioassay consisted of a two-step regeneration protocol adjusted and optimized for high-throughput manipulations of root explants of Arabidopsis (Arabidopsis thaliana) carrying the shoot regeneration marker LIGHT-DEPENDENT SHORT HYPOCOTYLS4. The screen revealed a single compound, the cytokinin-like phenyl-adenine (Phe-Ade), as a potent inducer of adventitious shoots. Although Phe-Ade triggered diverse cytokinin-dependent phenotypical responses, it did not inhibit shoot growth and was not cytotoxic at high concentrations. Transcript profiling of cytokinin-related genes revealed that Phe-Ade treatment established a typical cytokinin response. Moreover, Phe-Ade activated the cytokinin receptors ARABIDOPSIS HISTIDINE KINASE3 and ARABIDOPSIS HISTIDINE KINASE4 in a bacterial receptor assay, albeit at relatively high concentrations, illustrating that it exerts genuine but weak cytokinin activity. In addition, we demonstrated that Phe-Ade is a strong competitive inhibitor of CYTOKININ OXIDASE/DEHYDROGENASE enzymes, leading to an accumulation of endogenous cytokinins. Collectively, Phe-Ade exhibits a dual mode of action that results in a strong shoot-inducing activity. In vitro shoot regeneration is implemented in basic plant research and commercial plant production, but for some plant species, it is still difficult to achieve by means of the currently available cytokinins and auxins. To identify novel compounds that promote shoot regeneration, we screened a library of 10,000 small molecules. The bioassay consisted of a two-step regeneration protocol adjusted and optimized for high-throughput manipulations of root explants of Arabidopsis (Arabidopsis thaliana) carrying the shoot regeneration marker LIGHT-DEPENDENT SHORT HYPOCOTYLS4. The screen revealed a single compound, the cytokinin-like phenyl-adenine (Phe-Ade), as a potent inducer of adventitious shoots. Although Phe-Ade triggered diverse cytokinin-dependent phenotypical responses, it did not inhibit shoot growth and was not cytotoxic at high concentrations. Transcript profiling of cytokinin-related genes revealed that Phe-Ade treatment established a typical cytokinin response. Moreover, Phe-Ade activated the cytokinin receptors ARABIDOPSIS HISTIDINE KINASE3 and ARABIDOPSIS HISTIDINE KINASE4 in a bacterial receptor assay, albeit at relatively high concentrations, illustrating that it exerts genuine but weak cytokinin activity. In addition, we demonstrated that Phe-Ade is a strong competitive inhibitor of CYTOKININ OXIDASE/DEHYDROGENASE enzymes, leading to an accumulation of endogenous cytokinins. Collectively, Phe-Ade exhibits a dual mode of action that results in a strong shoot-inducing activity.
dcterms:title
Phenyl-adenine, identified in an LSH4-assisted chemical screen, is a potent compound for shoot regeneration through the inhibition of CKX activity Phenyl-adenine, identified in an LSH4-assisted chemical screen, is a potent compound for shoot regeneration through the inhibition of CKX activity
skos:prefLabel
Phenyl-adenine, identified in an LSH4-assisted chemical screen, is a potent compound for shoot regeneration through the inhibition of CKX activity Phenyl-adenine, identified in an LSH4-assisted chemical screen, is a potent compound for shoot regeneration through the inhibition of CKX activity
skos:notation
RIV/61989592:15310/13:33147814!RIV14-GA0-15310___
n10:predkladatel
n11:orjk%3A15310
n3:aktivita
n12:P
n3:aktivity
P(ED0007/01/01), P(GAP501/10/1141), P(GPP501/12/P160)
n3:cisloPeriodika
3
n3:dodaniDat
n16:2014
n3:domaciTvurceVysledku
n5:7890052 n5:7107544 n5:4481445 n5:4866029 n5:1711725 n5:1749560
n3:druhVysledku
n9:J
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n20:predkladatel
n3:idSjednocenehoVysledku
96044
n3:idVysledku
RIV/61989592:15310/13:33147814
n3:jazykVysledku
n21:eng
n3:klicovaSlova
inhibition of CKX activity; shoot regeneration; LSH4-assisted screen; Phenyl-adenine
n3:klicoveSlovo
n6:LSH4-assisted%20screen n6:Phenyl-adenine n6:inhibition%20of%20CKX%20activity n6:shoot%20regeneration
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[54FE0144F2F3]
n3:nazevZdroje
Plant Physiology
n3:obor
n19:GC
n3:pocetDomacichTvurcuVysledku
6
n3:pocetTvurcuVysledku
10
n3:projekt
n4:GPP501%2F12%2FP160 n4:GAP501%2F10%2F1141 n4:ED0007%2F01%2F01
n3:rokUplatneniVysledku
n16:2013
n3:svazekPeriodika
161
n3:tvurceVysledku
Plíhal, Ondřej Geelen, Danny Werbrouck, Stefaan Šmehilová, Mária Tarkowski, Petr Galuszka, Petr Vereecke, Danny Spíchal, Lukáš Jaworek, Pavel Motte, Hans
n3:wos
000316984100015
s:issn
0032-0889
s:numberOfPages
13
n7:doi
10.1104/pp.112.210716
n17:organizacniJednotka
15310