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Statements

Subject Item
n2:RIV%2F61989592%3A15310%2F13%3A33147707%21RIV14-GA0-15310___
rdf:type
skos:Concept n15:Vysledek
rdfs:seeAlso
http://onlinelibrary.wiley.com/doi/10.1002/elps.201300079/full
dcterms:description
The electrophoretic mobility shift assay (EMSA) is a method for the study of specific DNA-protein interactions in vitro. The pregnane X receptor (PRX) is a key xenobiotic sensor that regulates the expression of drug-metabolizing enzymes andmany other genes. Radiolabeled ??P-DNA-probes had been used in studies of PXR-DNA interactions. There is an increasing need for nonradioactive assays, due to the health, safety and environmental issues. In the current study, we present a protocol for the nonradioactive electrophoretic mobility shift assay, allowing studying interactions between human PXR with promoter DNA sequences. The electrophoretic mobility shift assay (EMSA) is a method for the study of specific DNA-protein interactions in vitro. The pregnane X receptor (PRX) is a key xenobiotic sensor that regulates the expression of drug-metabolizing enzymes andmany other genes. Radiolabeled ??P-DNA-probes had been used in studies of PXR-DNA interactions. There is an increasing need for nonradioactive assays, due to the health, safety and environmental issues. In the current study, we present a protocol for the nonradioactive electrophoretic mobility shift assay, allowing studying interactions between human PXR with promoter DNA sequences.
dcterms:title
A nonradioactive electrophoretic mobility shift assay for measurement of pregnane X receptor binding activity to CYP3A4 response element A nonradioactive electrophoretic mobility shift assay for measurement of pregnane X receptor binding activity to CYP3A4 response element
skos:prefLabel
A nonradioactive electrophoretic mobility shift assay for measurement of pregnane X receptor binding activity to CYP3A4 response element A nonradioactive electrophoretic mobility shift assay for measurement of pregnane X receptor binding activity to CYP3A4 response element
skos:notation
RIV/61989592:15310/13:33147707!RIV14-GA0-15310___
n15:predkladatel
n16:orjk%3A15310
n3:aktivita
n7:S n7:P
n3:aktivity
P(GA13-01809S), P(GAP303/12/0472), S
n3:cisloPeriodika
13
n3:dodaniDat
n5:2014
n3:domaciTvurceVysledku
n11:9933387 n11:4908171 n11:8069239
n3:druhVysledku
n6:J
n3:duvernostUdaju
n21:S
n3:entitaPredkladatele
n9:predkladatel
n3:idSjednocenehoVysledku
58870
n3:idVysledku
RIV/61989592:15310/13:33147707
n3:jazykVysledku
n10:eng
n3:klicovaSlova
Response elements; Pregnane X receptor; Electrophoretic mobility shift assay; Drug metabolism; Cytochrome P450
n3:klicoveSlovo
n4:Electrophoretic%20mobility%20shift%20assay n4:Response%20elements n4:Drug%20metabolism n4:Cytochrome%20P450 n4:Pregnane%20X%20receptor
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[D7AB5E358DB5]
n3:nazevZdroje
Electrophoresis
n3:obor
n19:FR
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:projekt
n18:GAP303%2F12%2F0472 n18:GA13-01809S
n3:rokUplatneniVysledku
n5:2013
n3:svazekPeriodika
34
n3:tvurceVysledku
Vávrová, Aneta Dvořák, Zdeněk Vrzal, Radim
n3:wos
000322112200003
s:issn
0173-0835
s:numberOfPages
6
n20:doi
10.1002/elps.201300079
n14:organizacniJednotka
15310