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Statements

Subject Item
n2:RIV%2F61989592%3A15310%2F13%3A33147385%21RIV15-MSM-15310___
rdf:type
n11:Vysledek skos:Concept
dcterms:description
The most important commercial and experimental approaches (excluding military instrumentation and biodefense applications) used in mass spectrometry for microorganism identification and evaluation or their biological properties were summarized in this Review. Some of them are the subject of intellectual property rights, as documented by about fifty patent applications published in CAPLUS database in past five years. The most recent (mainly Patent Cooperation Treaty, PCT) applications are reported on AST and virulence testing and were submitted by Bruker Daltonics, bioMe?rieux,Syft Technologies, and other private companies. Some vendors also protected their devices for sample preparation and robotics.Microorganism identification based on mass spectrometry of the amplicons has already been heavily patented by Ibis (Abbott). Typing of bacteria and yeasts has become standard, and significant progress has also been made in the identification of filamentous fungi. Identification can be finished in one minute if direct transfer of front mycelium is carried out. Analysis time is extended to 10 min when an ethanol extraction step is added. For slowly sporulating or in other way problematic fungi, a broth cultivation protocol taking one day was already developed. We conclude that the progressive role of mass spectrometry in microbiology109 and associated clinical areas has brought new users into the mass spectrometry field. In turn, the field is continuously being expanded due to its robustness, sensitivity, selectivity, speed, and vendor support and investments. In the future, we expect the broad expansion of mass spectrometry to biological and biochemical testing areas temporarily being occupied by conventional approaches. Routine food quality control and detection of infectious diseases including viral infections will soon become a reality. The most important commercial and experimental approaches (excluding military instrumentation and biodefense applications) used in mass spectrometry for microorganism identification and evaluation or their biological properties were summarized in this Review. Some of them are the subject of intellectual property rights, as documented by about fifty patent applications published in CAPLUS database in past five years. The most recent (mainly Patent Cooperation Treaty, PCT) applications are reported on AST and virulence testing and were submitted by Bruker Daltonics, bioMe?rieux,Syft Technologies, and other private companies. Some vendors also protected their devices for sample preparation and robotics.Microorganism identification based on mass spectrometry of the amplicons has already been heavily patented by Ibis (Abbott). Typing of bacteria and yeasts has become standard, and significant progress has also been made in the identification of filamentous fungi. Identification can be finished in one minute if direct transfer of front mycelium is carried out. Analysis time is extended to 10 min when an ethanol extraction step is added. For slowly sporulating or in other way problematic fungi, a broth cultivation protocol taking one day was already developed. We conclude that the progressive role of mass spectrometry in microbiology109 and associated clinical areas has brought new users into the mass spectrometry field. In turn, the field is continuously being expanded due to its robustness, sensitivity, selectivity, speed, and vendor support and investments. In the future, we expect the broad expansion of mass spectrometry to biological and biochemical testing areas temporarily being occupied by conventional approaches. Routine food quality control and detection of infectious diseases including viral infections will soon become a reality.
dcterms:title
Current Trends in Microbial Diagnostics Based on Mass Spectrometry Current Trends in Microbial Diagnostics Based on Mass Spectrometry
skos:prefLabel
Current Trends in Microbial Diagnostics Based on Mass Spectrometry Current Trends in Microbial Diagnostics Based on Mass Spectrometry
skos:notation
RIV/61989592:15310/13:33147385!RIV15-MSM-15310___
n3:aktivita
n5:I n5:P
n3:aktivity
I, P(ED2.1.00/03.0058), P(GAP206/12/1150), P(ME10013)
n3:cisloPeriodika
2
n3:dodaniDat
n12:2015
n3:domaciTvurceVysledku
n4:4140702 n4:4316851
n3:druhVysledku
n10:J
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
67556
n3:idVysledku
RIV/61989592:15310/13:33147385
n3:jazykVysledku
n9:eng
n3:klicovaSlova
amphotericin-B; cyclic-peptides; gas chromatography; routine identification; SIFT-MS; rapid detection; Aspergillus-fumigatus; clinical microbiology; MALDI-TOF-MS; desorption ionization-time
n3:klicoveSlovo
n8:desorption%20ionization-time n8:gas%20chromatography n8:amphotericin-B n8:SIFT-MS n8:cyclic-peptides n8:routine%20identification n8:clinical%20microbiology n8:rapid%20detection n8:Aspergillus-fumigatus n8:MALDI-TOF-MS
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[6489E4E6FA3F]
n3:nazevZdroje
Analytical Chemistry
n3:obor
n16:CB
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
3
n3:projekt
n13:GAP206%2F12%2F1150 n13:ED2.1.00%2F03.0058 n13:ME10013
n3:rokUplatneniVysledku
n12:2013
n3:svazekPeriodika
85
n3:tvurceVysledku
Havlíček, Vladimír Lemr, Karel Schug, Kevin A
n3:wos
000313668400020
s:issn
0003-2700
s:numberOfPages
8
n15:doi
10.1021/ac3031866
n17:organizacniJednotka
15310