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Statements

Subject Item
n2:RIV%2F61989592%3A15310%2F13%3A33147303%21RIV15-MSM-15310___
rdf:type
n12:Vysledek skos:Concept
dcterms:description
Due to their low polarities and dielectric constants, analytes in solvents such as hexane, chloroform, and ethyl acetate exhibit poor electrospray ionization (ESI) efficiency. These are deemed to be %22non-ESI-friendly%22 solvents. Continuous flow extractive desorption electrospray ionization (CF-EDESI) is a novel ambient ionization technique that was recently developed in our group to manipulate protein charge distributions. Here we demonstrate its potential for ionizing analytes from non-ESI-friendly solvents. This feature makes CF-EDESI attractive to the general analytical community due to its apparent potential in lipidomics, normal phase separations, and hyphenation of mass spectrometry with HPLC-NMR systems. In this context, interest was subsequently initiated to discern mechanistic aspects of CF-EDESI. To achieve this, mechanistic experiments associated with a seemingly similar ambient ionization technique, extractive electrospray ionization (EESI), were emulated to compare CF-EDESI and EESI. Analysis of a series of fatty acids in multiple solvents in the negative ionization mode revealed differences between the two techniques. Whereas EESI has been previously shown to operate via extraction of analytes into the spray solvent, data presented here for CF-EDESI point toward a liquid-liquid mixing process to facilitate ionization. Further, a partial factorial design experiment was performed to evaluate the effects of different experimental variables on signal intensity. Sample flow rate was confirmed to be among the most significant factors to affect sensitivity. As a whole, the work presented provides greater insight into a new ambient ionization process, which exhibits expanded capabilities over conventional ESI; in this case, for direct analysis from non-ESI-friendly solvents. Due to their low polarities and dielectric constants, analytes in solvents such as hexane, chloroform, and ethyl acetate exhibit poor electrospray ionization (ESI) efficiency. These are deemed to be %22non-ESI-friendly%22 solvents. Continuous flow extractive desorption electrospray ionization (CF-EDESI) is a novel ambient ionization technique that was recently developed in our group to manipulate protein charge distributions. Here we demonstrate its potential for ionizing analytes from non-ESI-friendly solvents. This feature makes CF-EDESI attractive to the general analytical community due to its apparent potential in lipidomics, normal phase separations, and hyphenation of mass spectrometry with HPLC-NMR systems. In this context, interest was subsequently initiated to discern mechanistic aspects of CF-EDESI. To achieve this, mechanistic experiments associated with a seemingly similar ambient ionization technique, extractive electrospray ionization (EESI), were emulated to compare CF-EDESI and EESI. Analysis of a series of fatty acids in multiple solvents in the negative ionization mode revealed differences between the two techniques. Whereas EESI has been previously shown to operate via extraction of analytes into the spray solvent, data presented here for CF-EDESI point toward a liquid-liquid mixing process to facilitate ionization. Further, a partial factorial design experiment was performed to evaluate the effects of different experimental variables on signal intensity. Sample flow rate was confirmed to be among the most significant factors to affect sensitivity. As a whole, the work presented provides greater insight into a new ambient ionization process, which exhibits expanded capabilities over conventional ESI; in this case, for direct analysis from non-ESI-friendly solvents.
dcterms:title
Continuous flow-extractive desorption electrospray ionization: Analysis from %22non-electrospray ionization-friendly%22 solvents and related mechanism Continuous flow-extractive desorption electrospray ionization: Analysis from %22non-electrospray ionization-friendly%22 solvents and related mechanism
skos:prefLabel
Continuous flow-extractive desorption electrospray ionization: Analysis from %22non-electrospray ionization-friendly%22 solvents and related mechanism Continuous flow-extractive desorption electrospray ionization: Analysis from %22non-electrospray ionization-friendly%22 solvents and related mechanism
skos:notation
RIV/61989592:15310/13:33147303!RIV15-MSM-15310___
n3:aktivita
n10:I n10:P
n3:aktivity
I, P(ED2.1.00/03.0058), P(GAP206/12/1150), P(ME10013)
n3:cisloPeriodika
MAR
n3:dodaniDat
n15:2015
n3:domaciTvurceVysledku
n19:4140702 n19:4316851
n3:druhVysledku
n9:J
n3:duvernostUdaju
n6:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
66893
n3:idVysledku
RIV/61989592:15310/13:33147303
n3:jazykVysledku
n11:eng
n3:klicovaSlova
Mass spectrometry; Progesterone; Electrospray; Ambient ionization
n3:klicoveSlovo
n4:Mass%20spectrometry n4:Electrospray n4:Ambient%20ionization n4:Progesterone
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[3BA0C245DDF2]
n3:nazevZdroje
Analytica Chimica Acta
n3:obor
n17:CB
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
5
n3:projekt
n14:ED2.1.00%2F03.0058 n14:GAP206%2F12%2F1150 n14:ME10013
n3:rokUplatneniVysledku
n15:2013
n3:svazekPeriodika
769
n3:tvurceVysledku
Schug, Kevin A Li, Li Lemr, Karel Yang, Samuel H Havlíček, Vladimír
n3:wos
000316591400011
s:issn
0003-2670
s:numberOfPages
7
n13:doi
10.1016/j.aca.2013.01.018
n18:organizacniJednotka
15310