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Statements

Subject Item
n2:RIV%2F61989592%3A15310%2F13%3A33145014%21RIV14-GA0-15310___
rdf:type
skos:Concept n9:Vysledek
dcterms:description
Methanogenic archaea produce methane as a metabolic product under anoxic conditions and they play a crucial role in the global methane cycle. In this study molecular diversity of methanogenic archaea in the hyporheic sediment of the lowland stream Sitka (Olomouc, Czech Republic) was analyzed by PCR amplification, cloning and sequencing analysis of the methyl coenzyme M reductase alpha subunit (mcrA) gene. Sequencing analysis of 60 clones revealed 24 different mcrA phylotypes from hyporheic sedimentary layers to a depth of 50 cm. Phylotypes were affiliated with Methanomicrobiales, Methanosarcinales and Methanobacteriales orders. Only one phylotype remains unclassified. The majority of the phylotypes showed higher affiliation with uncultured methanogens than with known methanogenic species. The presence of relatively rich assemblage of methanogenic archaea confirmed that methanogens may be an important component of hyporheic microbial communities and may affect CH4 cycling in rivers. Methanogenic archaea produce methane as a metabolic product under anoxic conditions and they play a crucial role in the global methane cycle. In this study molecular diversity of methanogenic archaea in the hyporheic sediment of the lowland stream Sitka (Olomouc, Czech Republic) was analyzed by PCR amplification, cloning and sequencing analysis of the methyl coenzyme M reductase alpha subunit (mcrA) gene. Sequencing analysis of 60 clones revealed 24 different mcrA phylotypes from hyporheic sedimentary layers to a depth of 50 cm. Phylotypes were affiliated with Methanomicrobiales, Methanosarcinales and Methanobacteriales orders. Only one phylotype remains unclassified. The majority of the phylotypes showed higher affiliation with uncultured methanogens than with known methanogenic species. The presence of relatively rich assemblage of methanogenic archaea confirmed that methanogens may be an important component of hyporheic microbial communities and may affect CH4 cycling in rivers.
dcterms:title
Identification of Methanogenic archaea in the Hyporheic Sediment of Sitka Stream Identification of Methanogenic archaea in the Hyporheic Sediment of Sitka Stream
skos:prefLabel
Identification of Methanogenic archaea in the Hyporheic Sediment of Sitka Stream Identification of Methanogenic archaea in the Hyporheic Sediment of Sitka Stream
skos:notation
RIV/61989592:15310/13:33145014!RIV14-GA0-15310___
n9:predkladatel
n13:orjk%3A15310
n3:aktivita
n20:P
n3:aktivity
P(GA526/09/1639)
n3:cisloPeriodika
11
n3:dodaniDat
n5:2014
n3:domaciTvurceVysledku
Chaudhary, Prem Prashant n8:7130139 n8:6531660 n8:1886886 n8:2756765 n8:7304609
n3:druhVysledku
n16:J
n3:duvernostUdaju
n7:S
n3:entitaPredkladatele
n11:predkladatel
n3:idSjednocenehoVysledku
78688
n3:idVysledku
RIV/61989592:15310/13:33145014
n3:jazykVysledku
n17:eng
n3:klicovaSlova
methanogenic archaea, methane, cloning, mcrA gene, hyporheic sediment
n3:klicoveSlovo
n10:hyporheic%20sediment n10:methanogenic%20archaea n10:methane n10:cloning n10:mcrA%20gene
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[9BAE6FFEE096]
n3:nazevZdroje
PLoS One
n3:obor
n19:EB
n3:pocetDomacichTvurcuVysledku
6
n3:pocetTvurcuVysledku
6
n3:projekt
n18:GA526%2F09%2F1639
n3:rokUplatneniVysledku
n5:2013
n3:svazekPeriodika
8
n3:tvurceVysledku
Buriánková, Iva Mach, Václav Brablcová, Lenka Chaudhary, Prem Prashant Rulík, Martin Dvořák, Petr
n3:wos
000327313100113
s:issn
1932-6203
s:numberOfPages
10
n14:doi
10.1371/journal.pone.0080804
n15:organizacniJednotka
15310