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Statements

Subject Item
n2:RIV%2F61989592%3A15310%2F12%3A33142476%21RIV13-MSM-15310___
rdf:type
skos:Concept n16:Vysledek
rdfs:seeAlso
http://www.sciencedirect.com/science/article/pii/S0176161712002593
dcterms:description
The effect of five topolins (meta-Topolin= mT; meta-Topolin riboside = mTR; meta-Methoxy topolin = MemT; meta-Methoxy topolin riboside = MemTR and 6-(meta-methoxy)-9-(tetrahydropyran-2-yl)-topolin = MemTTHP) on the photosynthetic pigments and leaf structures of micropropagated 'Williams' bananas was compared with the commonly used benzyladenine (BA). Surface-decontaminated explants were cultured for 70 d on modified Murashige and Skoog (MS) basal medium and supplemented with 10, 20 or 30 mu M cytokinins (CKs). At 10 d intervals, the photosynthetic pigments were quantified via spectrophotometric methods for 7 cycles. Generally, the maximum pigment content was attained between 40 and 50 d. The control plantlets had the highest pigment content (1150 mu g/g FW). Among the CKs, 10 mu M MemTTHP generally had the best pigment stimulatory effect at the same period. After 40 d, scanning electron microscopy (SEM) of the foliar surface showed that the stomata density was highest in 10 mu M MemTTHP-treated and lowest in 10 mu M MemTR-treated plantlets. The stomatal structure and pore area also varied with the type and concentration of CK added. Generally, prolonging culture duration as well as increasing CK concentrations reduced the pigment content. However, the drastic breakdown in chlorophyll pigments beyond 50 d was slightly inhibited by the presence of mT, mTR, MemTTHP and BA compared to the control. The CK-treated plantlets at equimolar concentration had comparable chlorophyll a/b and total chlorophyll/carotenoid ratios after 10 d; probably as an adaptive measure. At the end of the current study, 10 mu M mT and mTR plantlets remained green as reflected by the higher total chlorophyll/carotenoid ratio as well as by the visual observations. A well-developed photosynthetic apparatus enhances the survival of in vitro plantlets during the acclimatization stage. The effect of five topolins (meta-Topolin= mT; meta-Topolin riboside = mTR; meta-Methoxy topolin = MemT; meta-Methoxy topolin riboside = MemTR and 6-(meta-methoxy)-9-(tetrahydropyran-2-yl)-topolin = MemTTHP) on the photosynthetic pigments and leaf structures of micropropagated 'Williams' bananas was compared with the commonly used benzyladenine (BA). Surface-decontaminated explants were cultured for 70 d on modified Murashige and Skoog (MS) basal medium and supplemented with 10, 20 or 30 mu M cytokinins (CKs). At 10 d intervals, the photosynthetic pigments were quantified via spectrophotometric methods for 7 cycles. Generally, the maximum pigment content was attained between 40 and 50 d. The control plantlets had the highest pigment content (1150 mu g/g FW). Among the CKs, 10 mu M MemTTHP generally had the best pigment stimulatory effect at the same period. After 40 d, scanning electron microscopy (SEM) of the foliar surface showed that the stomata density was highest in 10 mu M MemTTHP-treated and lowest in 10 mu M MemTR-treated plantlets. The stomatal structure and pore area also varied with the type and concentration of CK added. Generally, prolonging culture duration as well as increasing CK concentrations reduced the pigment content. However, the drastic breakdown in chlorophyll pigments beyond 50 d was slightly inhibited by the presence of mT, mTR, MemTTHP and BA compared to the control. The CK-treated plantlets at equimolar concentration had comparable chlorophyll a/b and total chlorophyll/carotenoid ratios after 10 d; probably as an adaptive measure. At the end of the current study, 10 mu M mT and mTR plantlets remained green as reflected by the higher total chlorophyll/carotenoid ratio as well as by the visual observations. A well-developed photosynthetic apparatus enhances the survival of in vitro plantlets during the acclimatization stage.
dcterms:title
The role of meta-topolins on the photosynthetic pigment profiles and foliar structures of micropropagated 'Williams' bananas The role of meta-topolins on the photosynthetic pigment profiles and foliar structures of micropropagated 'Williams' bananas
skos:prefLabel
The role of meta-topolins on the photosynthetic pigment profiles and foliar structures of micropropagated 'Williams' bananas The role of meta-topolins on the photosynthetic pigment profiles and foliar structures of micropropagated 'Williams' bananas
skos:notation
RIV/61989592:15310/12:33142476!RIV13-MSM-15310___
n16:predkladatel
n17:orjk%3A15310
n4:aktivita
n5:P n5:Z
n4:aktivity
P(ED0007/01/01), P(GAP501/10/1450), P(QI92A247), Z(AV0Z50380511)
n4:cisloPeriodika
15
n4:dodaniDat
n10:2013
n4:domaciTvurceVysledku
n13:8147345 Van Staden, Johannes
n4:druhVysledku
n7:J
n4:duvernostUdaju
n19:S
n4:entitaPredkladatele
n21:predkladatel
n4:idSjednocenehoVysledku
166027
n4:idVysledku
RIV/61989592:15310/12:33142476
n4:jazykVysledku
n11:eng
n4:klicovaSlova
Musa spp; Microscopy; Micropropagation; Cytokinins; Chlorophylls; Carotenoids
n4:klicoveSlovo
n12:Micropropagation n12:Carotenoids n12:Chlorophylls n12:Microscopy n12:Cytokinins n12:Musa%20spp
n4:kodStatuVydavatele
DE - Spolková republika Německo
n4:kontrolniKodProRIV
[B6EE9B4BD792]
n4:nazevZdroje
Journal of Plant Physiology
n4:obor
n6:GK
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
5
n4:projekt
n15:GAP501%2F10%2F1450 n15:QI92A247 n15:ED0007%2F01%2F01
n4:rokUplatneniVysledku
n10:2012
n4:svazekPeriodika
169
n4:tvurceVysledku
Bairu, Michael W Szüčová, Lucie Van Staden, Johannes Aremu, Adeyemi O Finnie, Jeffrey F
n4:wos
000309566800011
n4:zamer
n22:AV0Z50380511
s:issn
0176-1617
s:numberOfPages
12
n20:doi
10.1016/j.jplph.2012.06.006
n18:organizacniJednotka
15310