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Statements

Subject Item
n2:RIV%2F61989592%3A15310%2F12%3A33142285%21RIV13-MSM-15310___
rdf:type
skos:Concept n12:Vysledek
dcterms:description
The rapid and simple ultra performance liquid chromatography-tandem mass spectrometry method was developed and validated for simultaneous determination parent drugs: sertraline, fluoxetine, citalopram, paroxetine, venlafaxine, clozapine, olanzapine, quetiapine, risperidone, and their active and nonacrive metabolites N-desmethylsertraline, norfluoxetine, desmethylcitalopram, didemethylcitalopram. N-desmethylvenlafaxine, O-desmethylvenlafaxine, N-desmethylclozapine, N-desmethylolanzapine, 2-hydroxyolanzapine and 9-hydroxyrisperidone in human serum. Precipitation of serum proteins was performed with a precipitation reagent consisting of 0.05% solution of ZnSO4 center dot 7H(2)O in acetonitrile/methanol (40:60, v/v). Alprenolol was used as an internal standard. Chromatographic separation was carried out on a BEH C18 column using gradient elution mobile phase A (2 mmol/L ammonium acetate, 0.1% formic acid in 5% acetonitrile, v/v/v) and B (2 mmol/L ammonium acetate, 0.1% formic acid in 95% acetonitrile, v/v/v). Electrospray in positive mode was used for ionization. Detection was performed on a triple-quadrupole tandem mass spectrometer by multiple reaction monitoring. Analysis time was 5 min. Drugs were separated into three groups with low, medium and high levels. Correlation coefficients of calibration curves were in the range 0.995-1.000. Coefficients of variation were 4.2-9.5% for intra-assay and 3.0-11.9% for inter-assay. Recoveries were 87.1-110% for intra-assay and 88.1-108.2% for inter-assay. The method was fully validated and can be successfully applied for routine analyses. The rapid and simple ultra performance liquid chromatography-tandem mass spectrometry method was developed and validated for simultaneous determination parent drugs: sertraline, fluoxetine, citalopram, paroxetine, venlafaxine, clozapine, olanzapine, quetiapine, risperidone, and their active and nonacrive metabolites N-desmethylsertraline, norfluoxetine, desmethylcitalopram, didemethylcitalopram. N-desmethylvenlafaxine, O-desmethylvenlafaxine, N-desmethylclozapine, N-desmethylolanzapine, 2-hydroxyolanzapine and 9-hydroxyrisperidone in human serum. Precipitation of serum proteins was performed with a precipitation reagent consisting of 0.05% solution of ZnSO4 center dot 7H(2)O in acetonitrile/methanol (40:60, v/v). Alprenolol was used as an internal standard. Chromatographic separation was carried out on a BEH C18 column using gradient elution mobile phase A (2 mmol/L ammonium acetate, 0.1% formic acid in 5% acetonitrile, v/v/v) and B (2 mmol/L ammonium acetate, 0.1% formic acid in 95% acetonitrile, v/v/v). Electrospray in positive mode was used for ionization. Detection was performed on a triple-quadrupole tandem mass spectrometer by multiple reaction monitoring. Analysis time was 5 min. Drugs were separated into three groups with low, medium and high levels. Correlation coefficients of calibration curves were in the range 0.995-1.000. Coefficients of variation were 4.2-9.5% for intra-assay and 3.0-11.9% for inter-assay. Recoveries were 87.1-110% for intra-assay and 88.1-108.2% for inter-assay. The method was fully validated and can be successfully applied for routine analyses.
dcterms:title
Liquid chromatography-tandem mass spectrometry method for determination of five antidepressants and four atypical antipsychotics and their main metabolites in human serum Liquid chromatography-tandem mass spectrometry method for determination of five antidepressants and four atypical antipsychotics and their main metabolites in human serum
skos:prefLabel
Liquid chromatography-tandem mass spectrometry method for determination of five antidepressants and four atypical antipsychotics and their main metabolites in human serum Liquid chromatography-tandem mass spectrometry method for determination of five antidepressants and four atypical antipsychotics and their main metabolites in human serum
skos:notation
RIV/61989592:15310/12:33142285!RIV13-MSM-15310___
n12:predkladatel
n13:orjk%3A15310
n3:aktivita
n16:V n16:P
n3:aktivity
P(ED2.1.00/03.0058), V
n3:cisloPeriodika
říjen
n3:dodaniDat
n11:2013
n3:domaciTvurceVysledku
n4:9956034 n4:4316851
n3:druhVysledku
n19:J
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n8:predkladatel
n3:idSjednocenehoVysledku
147180
n3:idVysledku
RIV/61989592:15310/12:33142285
n3:jazykVysledku
n5:eng
n3:klicovaSlova
Mass spectrometry; High performance liquid chromatography; Therapeutic drug monitoring; Psychotropic drugs
n3:klicoveSlovo
n15:Psychotropic%20drugs n15:Mass%20spectrometry n15:High%20performance%20liquid%20chromatography n15:Therapeutic%20drug%20monitoring
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[1EDFA1636D04]
n3:nazevZdroje
Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
n3:obor
n14:CB
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
7
n3:projekt
n18:ED2.1.00%2F03.0058
n3:rokUplatneniVysledku
n11:2012
n3:svazekPeriodika
907
n3:tvurceVysledku
Brozmanová, Hana Grundmann, Milan Lemr, Karel Šištík, Pavel Kacířová, Ivana Uřinovská, Romana Šilhán, Petr
n3:wos
000310256500015
s:issn
1570-0232
s:numberOfPages
7
n20:doi
10.1016/j.jchromb.2012.09.009
n7:organizacniJednotka
15310