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Statements

Subject Item
n2:RIV%2F61989592%3A15310%2F12%3A33138883%21RIV13-MSM-15310___
rdf:type
n3:Vysledek skos:Concept
dcterms:description
Liquid chromatography on various stationary phases was used for the metabolite profile analysis of quercetin, rutin, isoquercitrin and taxifolin. The metabolites were obtained using an in vitro model system of human and rat hepatocytes in the form of cell suspensions and the primary cultures. For separations of the parent compounds and their metabolites, stationary phases based on C18, C8, cyanopropyl or phenyl modifications of silica were tested. Individual metabolites were identified on the basis of an elemental composition determination using electrospray ionization-quadrupole time-of-flight mass spectrometry (ESI-QqTOF MS) on-line connected with an LC system. The predominant metabolites were methylated flavonols and flavonol glucuronides. The highest biotransformation rate was found with taxifolin, which was mainly converted to sulfates. The HPLC/ESI-QqTOF MS analyses revealed that quercetin and taxifolin were metabolized more extensively than the studied glycosides, rutin and isoquercitrin. Liquid chromatography on various stationary phases was used for the metabolite profile analysis of quercetin, rutin, isoquercitrin and taxifolin. The metabolites were obtained using an in vitro model system of human and rat hepatocytes in the form of cell suspensions and the primary cultures. For separations of the parent compounds and their metabolites, stationary phases based on C18, C8, cyanopropyl or phenyl modifications of silica were tested. Individual metabolites were identified on the basis of an elemental composition determination using electrospray ionization-quadrupole time-of-flight mass spectrometry (ESI-QqTOF MS) on-line connected with an LC system. The predominant metabolites were methylated flavonols and flavonol glucuronides. The highest biotransformation rate was found with taxifolin, which was mainly converted to sulfates. The HPLC/ESI-QqTOF MS analyses revealed that quercetin and taxifolin were metabolized more extensively than the studied glycosides, rutin and isoquercitrin.
dcterms:title
Biotransformation of flavonols and taxifolin in hepatocyte in vitro systems as determined by liquid chromatography with various stationary phases and electrospray ionization-quadrupole time-of-flight mass spectrometry Biotransformation of flavonols and taxifolin in hepatocyte in vitro systems as determined by liquid chromatography with various stationary phases and electrospray ionization-quadrupole time-of-flight mass spectrometry
skos:prefLabel
Biotransformation of flavonols and taxifolin in hepatocyte in vitro systems as determined by liquid chromatography with various stationary phases and electrospray ionization-quadrupole time-of-flight mass spectrometry Biotransformation of flavonols and taxifolin in hepatocyte in vitro systems as determined by liquid chromatography with various stationary phases and electrospray ionization-quadrupole time-of-flight mass spectrometry
skos:notation
RIV/61989592:15310/12:33138883!RIV13-MSM-15310___
n3:predkladatel
n18:orjk%3A15310
n4:aktivita
n13:S n13:P
n4:aktivity
P(ED2.1.00/03.0058), P(GAP301/11/0767), S
n4:cisloPeriodika
15.6.2012
n4:dodaniDat
n7:2013
n4:domaciTvurceVysledku
n16:3649768
n4:druhVysledku
n11:J
n4:duvernostUdaju
n17:S
n4:entitaPredkladatele
n10:predkladatel
n4:idSjednocenehoVysledku
125201
n4:idVysledku
RIV/61989592:15310/12:33138883
n4:jazykVysledku
n8:eng
n4:klicovaSlova
hepatocytes; mass spektrometry; retention behaviour; liquid chromatography; metabolism; taxifolin; flavanonol; Flavonols
n4:klicoveSlovo
n5:liquid%20chromatography n5:retention%20behaviour n5:hepatocytes n5:taxifolin n5:metabolism n5:mass%20spektrometry n5:flavanonol n5:Flavonols
n4:kodStatuVydavatele
NL - Nizozemsko
n4:kontrolniKodProRIV
[5A55B742C331]
n4:nazevZdroje
JOURNAL OF CHROMATOGRAPHY B
n4:obor
n9:CE
n4:pocetDomacichTvurcuVysledku
1
n4:pocetTvurcuVysledku
6
n4:projekt
n14:GAP301%2F11%2F0767 n14:ED2.1.00%2F03.0058
n4:rokUplatneniVysledku
n7:2012
n4:svazekPeriodika
899
n4:tvurceVysledku
Papoušková, Barbora Vrba, Jiří Křen, Vladimír Ulrichová, Jitka Vacek, Jan Kosina, Pavel
s:issn
1570-0232
s:numberOfPages
7
n19:organizacniJednotka
15310