This HTML5 document contains 55 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n19http://localhost/temp/predkladatel/
n21http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n15http://linked.opendata.cz/resource/domain/vavai/subjekt/
n12http://linked.opendata.cz/ontology/domain/vavai/
n16http://linked.opendata.cz/resource/domain/vavai/zamer/
n9http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F61989592%3A15310%2F11%3A33148777%21RIV14-MSM-15310___/
shttp://schema.org/
skoshttp://www.w3.org/2004/02/skos/core#
rdfshttp://www.w3.org/2000/01/rdf-schema#
n3http://linked.opendata.cz/ontology/domain/vavai/riv/
n4http://bibframe.org/vocab/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n7http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n14http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n18http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n5http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n20http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n13http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n10http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F61989592%3A15310%2F11%3A33148777%21RIV14-MSM-15310___
rdf:type
n12:Vysledek skos:Concept
rdfs:seeAlso
http://nar.oxfordjournals.org/content/39/6/2330
dcterms:description
Argonaute (Ago) proteins are highly conserved between species and constitute a direct-binding platform for small RNAs including short-interfering RNAs (siRNAs), microRNAs (miRNAs) and Piwi interacting RNAs (piRNAs). Small RNAs function as guides whereas Ago proteins are the actual mediators of gene silencing. Although the major steps in RNA-guided gene silencing have been elucidated, not much is known about Ago-protein regulation. Here we report a comprehensive analysis of Ago2 phosphorylation in human cells. We find that the highly conserved tyrosine Y529, located in the small RNA 5'-end-binding pocket of Ago proteins can be phosphorylated. By substituting Y529 with a negatively charged glutamate (E) mimicking a phosphorylated tyrosine, we show that small RNA binding is strongly reduced. Our data suggest that a negatively charged phospho-tyrosine generates a repulsive force that prevents efficient binding of the negatively charged 5' phosphate of the small RNA Argonaute (Ago) proteins are highly conserved between species and constitute a direct-binding platform for small RNAs including short-interfering RNAs (siRNAs), microRNAs (miRNAs) and Piwi interacting RNAs (piRNAs). Small RNAs function as guides whereas Ago proteins are the actual mediators of gene silencing. Although the major steps in RNA-guided gene silencing have been elucidated, not much is known about Ago-protein regulation. Here we report a comprehensive analysis of Ago2 phosphorylation in human cells. We find that the highly conserved tyrosine Y529, located in the small RNA 5'-end-binding pocket of Ago proteins can be phosphorylated. By substituting Y529 with a negatively charged glutamate (E) mimicking a phosphorylated tyrosine, we show that small RNA binding is strongly reduced. Our data suggest that a negatively charged phospho-tyrosine generates a repulsive force that prevents efficient binding of the negatively charged 5' phosphate of the small RNA
dcterms:title
Phosphorylation of human Argonaute proteins affects small RNA binding Phosphorylation of human Argonaute proteins affects small RNA binding
skos:prefLabel
Phosphorylation of human Argonaute proteins affects small RNA binding Phosphorylation of human Argonaute proteins affects small RNA binding
skos:notation
RIV/61989592:15310/11:33148777!RIV14-MSM-15310___
n12:predkladatel
n15:orjk%3A15310
n3:aktivita
n18:Z
n3:aktivity
Z(MSM6198959216)
n3:cisloPeriodika
6
n3:dodaniDat
n10:2014
n3:domaciTvurceVysledku
n21:9896821
n3:druhVysledku
n20:J
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n9:predkladatel
n3:idSjednocenehoVysledku
220217
n3:idVysledku
RIV/61989592:15310/11:33148777
n3:jazykVysledku
n5:eng
n3:klicovaSlova
cleavage; mechanisms; mirnas; microrna; P-bodies; piwi-domain; messenger-RNAs; crystal-structure; aeolicus argonaute; silencing complex
n3:klicoveSlovo
n7:aeolicus%20argonaute n7:messenger-RNAs n7:cleavage n7:mechanisms n7:microrna n7:crystal-structure n7:piwi-domain n7:mirnas n7:P-bodies n7:silencing%20complex
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[AFAC04EC98F5]
n3:nazevZdroje
Nucleic Acids Research
n3:obor
n13:EB
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
8
n3:rokUplatneniVysledku
n10:2011
n3:svazekPeriodika
39
n3:tvurceVysledku
Meister, Gunter Lenobel, René Wang, Yanli Hsiao, He-Hsuan Patel, Dinshaw Rüdel, Sabine Urlaub, Henning Körner, Roman
n3:wos
000289166400037
n3:zamer
n16:MSM6198959216
s:issn
0305-1048
s:numberOfPages
14
n4:doi
10.1093/nar/gkq1032
n19:organizacniJednotka
15310