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Statements

Subject Item
n2:RIV%2F61989592%3A15310%2F11%3A33119786%21RIV12-MSM-15310___
rdf:type
n7:Vysledek skos:Concept
dcterms:description
The presence of light, oxygen and photosensitizer (organic dye) is required for the photodynamic effect. Light and photosensitizer are harmless by themselves, butwhen combinedwith oxygen, reactive oxygen species (ROS) can be produced. This photodynamic effect is used in photodynamic therapy (PDT); the production of ROS as lethal cytotoxic agents can inactivate tumor cells. However, during PDT, there aremany difficulties, so it is not possible to excite the photosensitizer using a laser, a source of light at the wavelengths specific to the photosensitizer (in visible region of the spectrum). Chemiluminescence is the light emission as a result of a chemical reaction. It is possible to use a chemiluminescent mixture to excite the photosensitizer even if the light emission does not conform to the absorption maximum of the photosensitizer. Luciferin and luminol have been used as chemiluminescent compounds (energizers) for the excitation of the photosensitizers.The aimof this workwas to compare the chemiexcitation of some selected photosensitizers (e.g. fluorescein, eosin,methylene blue, hypericin and phthalocyanines) by chemiluminescent mixtures containing luminol (high chemiluminescent quantum yield) or phthalhydrazide (low chemiluminescent quantum yield) on some Gram-positive (Enterococcus faecalis, Staphylococcus aureus) and Gram-negative (Pseudomonas aeruginosa, E. coli) bacteria and some cell lines (NIH3T3 and MCF7). The efficiency of the chemiexcitation was dependent on the kind of the photosensitizer and on the type of the bacterial strain or cell line and was independent of the energizers. The presence of light, oxygen and photosensitizer (organic dye) is required for the photodynamic effect. Light and photosensitizer are harmless by themselves, butwhen combinedwith oxygen, reactive oxygen species (ROS) can be produced. This photodynamic effect is used in photodynamic therapy (PDT); the production of ROS as lethal cytotoxic agents can inactivate tumor cells. However, during PDT, there aremany difficulties, so it is not possible to excite the photosensitizer using a laser, a source of light at the wavelengths specific to the photosensitizer (in visible region of the spectrum). Chemiluminescence is the light emission as a result of a chemical reaction. It is possible to use a chemiluminescent mixture to excite the photosensitizer even if the light emission does not conform to the absorption maximum of the photosensitizer. Luciferin and luminol have been used as chemiluminescent compounds (energizers) for the excitation of the photosensitizers.The aimof this workwas to compare the chemiexcitation of some selected photosensitizers (e.g. fluorescein, eosin,methylene blue, hypericin and phthalocyanines) by chemiluminescent mixtures containing luminol (high chemiluminescent quantum yield) or phthalhydrazide (low chemiluminescent quantum yield) on some Gram-positive (Enterococcus faecalis, Staphylococcus aureus) and Gram-negative (Pseudomonas aeruginosa, E. coli) bacteria and some cell lines (NIH3T3 and MCF7). The efficiency of the chemiexcitation was dependent on the kind of the photosensitizer and on the type of the bacterial strain or cell line and was independent of the energizers.
dcterms:title
The photodynamic effect: the comparison of chemiexcitation by luminol and phthalhydrazide The photodynamic effect: the comparison of chemiexcitation by luminol and phthalhydrazide
skos:prefLabel
The photodynamic effect: the comparison of chemiexcitation by luminol and phthalhydrazide The photodynamic effect: the comparison of chemiexcitation by luminol and phthalhydrazide
skos:notation
RIV/61989592:15310/11:33119786!RIV12-MSM-15310___
n7:predkladatel
n8:orjk%3A15310
n3:aktivita
n15:Z
n3:aktivity
Z(MSM6198959216)
n3:cisloPeriodika
6
n3:dodaniDat
n18:2012
n3:domaciTvurceVysledku
n5:5964423 n5:3373215
n3:druhVysledku
n9:J
n3:duvernostUdaju
n12:S
n3:entitaPredkladatele
n6:predkladatel
n3:idSjednocenehoVysledku
220244
n3:idVysledku
RIV/61989592:15310/11:33119786
n3:jazykVysledku
n19:eng
n3:klicovaSlova
cell line; bacterium; phthalhydrazide; luminol; photodynamic effect
n3:klicoveSlovo
n4:photodynamic%20effect n4:cell%20line n4:luminol n4:bacterium n4:phthalhydrazide
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[239A49D5D193]
n3:nazevZdroje
Luminescence
n3:obor
n20:CF
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
2
n3:rokUplatneniVysledku
n18:2011
n3:svazekPeriodika
26
n3:tvurceVysledku
Lasovský, Jan Bancířová, Martina
n3:wos
000297923800005
n3:zamer
n13:MSM6198959216
s:issn
1522-7235
s:numberOfPages
6
n11:doi
10.1002/bio.1245
n17:organizacniJednotka
15310