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Statements

Subject Item
n2:RIV%2F61989592%3A15310%2F10%3A33115951%21RIV12-MSM-15310___
rdf:type
skos:Concept n6:Vysledek
dcterms:description
The present study was conducted to examine changes in photosynthetic pigment composition and functional state of the thylakoid membranes during the individual steps of preparation of samples that are intended for a separation of pigmentprotein complexes by nondenaturing polyacrylamide gel electrophoresis. The thylakoid membranes were isolated from barley leaves (Hordeum vulgare L.) grown under low irradiance (50 mýmol m-2 s-1). Functional state of the thylakoid membrane preparations was evaluated by determination of the maximal photochemical efficiency of photosystem (PS) II (FV/FM) and by analysis of excitation and emission spectra of chlorophyll a (Chl a) fluorescence at 77 K. All measurements were done at three phases of preparation of the samples: (1) in the suspensions of osmotically-shocked broken chloroplasts, (2) thylakoid membranes in extraction buffer containing Tris, glycine, and glycerol and (3) thylakoid membranes solubilized with a detergent decyl-beta-D-maltosid. FV/FM was reduced from 0.815 in the first step to 0.723 in the second step and to values close to zero in solubilized membranes. Pigment composition was not pronouncedly changed during preparation of the thylakoid membrane samples. Isolation of thylakoid membranes affected the efficiency of excitation energy transfer within PSII complexes only slightly. Emission and excitation fluorescence spectra of the solubilized membranes resemble spectra of trimers of PSII light-harvesting complexes (LHCII). Despite a disrupted excitation energy transfer from LHCII to PSII antenna core in solubilized membranes, energy transfer from Chl b and carotenoids to emission forms of Chl a within LHCII trimers remained effective. The present study was conducted to examine changes in photosynthetic pigment composition and functional state of the thylakoid membranes during the individual steps of preparation of samples that are intended for a separation of pigmentprotein complexes by nondenaturing polyacrylamide gel electrophoresis. The thylakoid membranes were isolated from barley leaves (Hordeum vulgare L.) grown under low irradiance (50 mýmol m-2 s-1). Functional state of the thylakoid membrane preparations was evaluated by determination of the maximal photochemical efficiency of photosystem (PS) II (FV/FM) and by analysis of excitation and emission spectra of chlorophyll a (Chl a) fluorescence at 77 K. All measurements were done at three phases of preparation of the samples: (1) in the suspensions of osmotically-shocked broken chloroplasts, (2) thylakoid membranes in extraction buffer containing Tris, glycine, and glycerol and (3) thylakoid membranes solubilized with a detergent decyl-beta-D-maltosid. FV/FM was reduced from 0.815 in the first step to 0.723 in the second step and to values close to zero in solubilized membranes. Pigment composition was not pronouncedly changed during preparation of the thylakoid membrane samples. Isolation of thylakoid membranes affected the efficiency of excitation energy transfer within PSII complexes only slightly. Emission and excitation fluorescence spectra of the solubilized membranes resemble spectra of trimers of PSII light-harvesting complexes (LHCII). Despite a disrupted excitation energy transfer from LHCII to PSII antenna core in solubilized membranes, energy transfer from Chl b and carotenoids to emission forms of Chl a within LHCII trimers remained effective.
dcterms:title
Pigment composition and functional state of the thylakoid membranes during preparation of samples for pigment-protein complexes separation by nondenaturing gel electrophoresis. Pigment composition and functional state of the thylakoid membranes during preparation of samples for pigment-protein complexes separation by nondenaturing gel electrophoresis.
skos:prefLabel
Pigment composition and functional state of the thylakoid membranes during preparation of samples for pigment-protein complexes separation by nondenaturing gel electrophoresis. Pigment composition and functional state of the thylakoid membranes during preparation of samples for pigment-protein complexes separation by nondenaturing gel electrophoresis.
skos:notation
RIV/61989592:15310/10:33115951!RIV12-MSM-15310___
n3:aktivita
n10:Z n10:S n10:P
n3:aktivity
P(GP522/07/P246), S, Z(MSM6198959215)
n3:cisloPeriodika
3
n3:dodaniDat
n9:2012
n3:domaciTvurceVysledku
n14:3731596
n3:druhVysledku
n16:J
n3:duvernostUdaju
n4:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
278981
n3:idVysledku
RIV/61989592:15310/10:33115951
n3:jazykVysledku
n13:eng
n3:klicovaSlova
thylakoid membranes isolation; polyacrylamide gel electrophoresis; photochemical efficiency; Hordeum vulgare; 77-K chlorophyll fluorescence spectra
n3:klicoveSlovo
n7:polyacrylamide%20gel%20electrophoresis n7:Hordeum%20vulgare n7:77-K%20chlorophyll%20fluorescence%20spectra n7:photochemical%20efficiency n7:thylakoid%20membranes%20isolation
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[B9EDC2E360EE]
n3:nazevZdroje
Photosynthetica
n3:obor
n18:BO
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
6
n3:projekt
n17:GP522%2F07%2FP246
n3:rokUplatneniVysledku
n9:2010
n3:svazekPeriodika
48
n3:tvurceVysledku
Štroch, Michal Karlický, Václav Špunda, Vladimír Čajánek, Martin Nadkanská, Lenka Podolínská, Jana
n3:zamer
n19:MSM6198959215
s:issn
0300-3604
s:numberOfPages
6
n11:organizacniJednotka
15310