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Statements

Subject Item
n2:RIV%2F61989592%3A15310%2F10%3A10212711%21RIV11-MSM-15310___
rdf:type
skos:Concept n16:Vysledek
dcterms:description
Protoplast cultures are remarkable examples of plant cell dedifferentiation. The state of dedifferentiation is evidenced by changes in cell morphology, genome organization, as well as by the capability of protoplasts to differentiate into multiple types of cells. The first change in the genome structure is connected with large-scale chromatin decondensation, affecting chromocenters involving various types of these repetitive sequences. This paper describes not only the de- and re-condensation of repetitive sequences, but it also compares the recondensation level of chromatin with the levels of oxidative stress which were decreased by using an antioxidant. We have demonstrated that the treatment of protoplasts with ascorbic acid not only decreased the level of oxidative stress but also positively stimulated the expression of the ascorbate peroxidase and catalase. It also led to a greater recondensation of the chromatin (when compared to the untreated protoplasts). Protoplast cultures are remarkable examples of plant cell dedifferentiation. The state of dedifferentiation is evidenced by changes in cell morphology, genome organization, as well as by the capability of protoplasts to differentiate into multiple types of cells. The first change in the genome structure is connected with large-scale chromatin decondensation, affecting chromocenters involving various types of these repetitive sequences. This paper describes not only the de- and re-condensation of repetitive sequences, but it also compares the recondensation level of chromatin with the levels of oxidative stress which were decreased by using an antioxidant. We have demonstrated that the treatment of protoplasts with ascorbic acid not only decreased the level of oxidative stress but also positively stimulated the expression of the ascorbate peroxidase and catalase. It also led to a greater recondensation of the chromatin (when compared to the untreated protoplasts).
dcterms:title
Recondensation level of repetitive sequences in the plant protoplast nucleus is limited by oxidative stress Recondensation level of repetitive sequences in the plant protoplast nucleus is limited by oxidative stress
skos:prefLabel
Recondensation level of repetitive sequences in the plant protoplast nucleus is limited by oxidative stress Recondensation level of repetitive sequences in the plant protoplast nucleus is limited by oxidative stress
skos:notation
RIV/61989592:15310/10:10212711!RIV11-MSM-15310___
n3:aktivita
n15:P n15:Z
n3:aktivity
P(2E08018), Z(MSM6198959215)
n3:cisloPeriodika
9
n3:dodaniDat
n9:2011
n3:domaciTvurceVysledku
n4:2025167 n4:1301411 n4:2584913 n4:2038269 n4:4790782 n4:8054312 n4:5930731
n3:druhVysledku
n10:J
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
284206
n3:idVysledku
RIV/61989592:15310/10:10212711
n3:jazykVysledku
n6:eng
n3:klicovaSlova
protoplasts; oxidative stress; nuclear organization; chromatin
n3:klicoveSlovo
n11:protoplasts n11:oxidative%20stress n11:nuclear%20organization n11:chromatin
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[8DBDAE9EE3C1]
n3:nazevZdroje
Journal of Experimenal Botany
n3:obor
n13:EF
n3:pocetDomacichTvurcuVysledku
7
n3:pocetTvurcuVysledku
7
n3:projekt
n12:2E08018
n3:rokUplatneniVysledku
n9:2010
n3:svazekPeriodika
61
n3:tvurceVysledku
Luhová, Lenka Protivánková, Iva Piterková, Jana Lebeda, Aleš Ondřej, Vladan Navrátilová, Božena Sedlářová, Michaela
n3:wos
000279003600015
n3:zamer
n17:MSM6198959215
s:issn
0022-0957
s:numberOfPages
7
n5:organizacniJednotka
15310