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Statements

Subject Item
n2:RIV%2F61989592%3A15310%2F10%3A10212423%21RIV11-MSM-15310___
rdf:type
n7:Vysledek skos:Concept
dcterms:description
Electron paramagnetic resonance (EPR) spin trapping spectroscopy is an important method used in free radical research; however, its application in biological systems is hindered by EPR silencing of spin adducts. Previous studies in superoxide-generating chemical systems have shown that spin adducts can be partially stabilized by cyclodextrins. In this work, for the first time, this proposed protective effect of cyclodextrins is investigated in a real biological sample?in isolated thylakoid membranes and photosystem II (PSII) particles with EMPO as a spin trap. Electron paramagnetic resonance (EPR) spin trapping spectroscopy is an important method used in free radical research; however, its application in biological systems is hindered by EPR silencing of spin adducts. Previous studies in superoxide-generating chemical systems have shown that spin adducts can be partially stabilized by cyclodextrins. In this work, for the first time, this proposed protective effect of cyclodextrins is investigated in a real biological sample?in isolated thylakoid membranes and photosystem II (PSII) particles with EMPO as a spin trap.
dcterms:title
Improvement of the sensitivity of EPR spin trapping in biological systems by cyclodextrins: A model study with thylakoids and photosystem II particles Improvement of the sensitivity of EPR spin trapping in biological systems by cyclodextrins: A model study with thylakoids and photosystem II particles
skos:prefLabel
Improvement of the sensitivity of EPR spin trapping in biological systems by cyclodextrins: A model study with thylakoids and photosystem II particles Improvement of the sensitivity of EPR spin trapping in biological systems by cyclodextrins: A model study with thylakoids and photosystem II particles
skos:notation
RIV/61989592:15310/10:10212423!RIV11-MSM-15310___
n3:aktivita
n14:Z
n3:aktivity
Z(MSM6198959215)
n3:cisloPeriodika
2
n3:dodaniDat
n6:2011
n3:domaciTvurceVysledku
n13:1466119
n3:druhVysledku
n10:J
n3:duvernostUdaju
n12:S
n3:entitaPredkladatele
n8:predkladatel
n3:idSjednocenehoVysledku
263199
n3:idVysledku
RIV/61989592:15310/10:10212423
n3:jazykVysledku
n18:eng
n3:klicovaSlova
Free radicals; Chlorophyll fluorescence induction; Superoxide; Photosystem II; Thylakoid; EMPO; Spin adduct stability; Electron paramagnetic resonance; Spin trapping; 2-Hydroxypropyl-beta-cyclodextrin; Randomly methylated-beta-cyclodextrin; Cyclodextrin
n3:klicoveSlovo
n4:Superoxide n4:Free%20radicals n4:Spin%20adduct%20stability n4:Chlorophyll%20fluorescence%20induction n4:Electron%20paramagnetic%20resonance n4:Spin%20trapping n4:Photosystem%20II n4:Thylakoid n4:Randomly%20methylated-beta-cyclodextrin n4:Cyclodextrin n4:EMPO n4:2-Hydroxypropyl-beta-cyclodextrin
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[67D2C625C211]
n3:nazevZdroje
Free Radical Biology and Medicine
n3:obor
n17:BO
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
1
n3:rokUplatneniVysledku
n6:2010
n3:svazekPeriodika
48
n3:tvurceVysledku
Šnyrychová, Iva
n3:wos
000273883800010
n3:zamer
n16:MSM6198959215
s:issn
0891-5849
s:numberOfPages
11
n15:organizacniJednotka
15310