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Statements

Subject Item
n2:RIV%2F61989592%3A15310%2F10%3A10211974%21RIV11-MZE-15310___
rdf:type
skos:Concept n14:Vysledek
dcterms:description
Traditional plant breeding relies upon crosses and subsequent selection of genotypes to meet desirable traits. The incorporation of marker-assisted selection (MAS) into breeding strategies would result in a reduction in the number of offspring to be propagated, selected and tested. In the case of pea (Pisum sativum L.), the testing of resistance to viral pathogens such as Pea seed-borne mosaic virus (PSbMV) is included in the breeding process. Resistance to the common strains of PSbMV is conferred by a single recessive gene (eIF4E), localized on LG VI (sbm-1 locus). We have analyzed for variation in the eIF4E genomic sequences from 43 pea varieties and breeding lines, reported as donors of resistance. This enabled a comprehensive investigation of the eIF4E gene structure and mutations responsible for PSbMV resistance were identified. Subsequently, PCR-based and gene-specific single nucleotide polymorphism (SNP) and co-dominant amplicon length polymorphism markers were developed. Traditional plant breeding relies upon crosses and subsequent selection of genotypes to meet desirable traits. The incorporation of marker-assisted selection (MAS) into breeding strategies would result in a reduction in the number of offspring to be propagated, selected and tested. In the case of pea (Pisum sativum L.), the testing of resistance to viral pathogens such as Pea seed-borne mosaic virus (PSbMV) is included in the breeding process. Resistance to the common strains of PSbMV is conferred by a single recessive gene (eIF4E), localized on LG VI (sbm-1 locus). We have analyzed for variation in the eIF4E genomic sequences from 43 pea varieties and breeding lines, reported as donors of resistance. This enabled a comprehensive investigation of the eIF4E gene structure and mutations responsible for PSbMV resistance were identified. Subsequently, PCR-based and gene-specific single nucleotide polymorphism (SNP) and co-dominant amplicon length polymorphism markers were developed.
dcterms:title
Marker assisted pea breeding: eIF4E allele specific markers to Pea seed-borne mosaic virus Marker assisted pea breeding: eIF4E allele specific markers to Pea seed-borne mosaic virus
skos:prefLabel
Marker assisted pea breeding: eIF4E allele specific markers to Pea seed-borne mosaic virus Marker assisted pea breeding: eIF4E allele specific markers to Pea seed-borne mosaic virus
skos:notation
RIV/61989592:15310/10:10211974!RIV11-MZE-15310___
n3:aktivita
n7:Z n7:S n7:P
n3:aktivity
P(LA08011), P(QI91A229), S, Z(MSM2678424601)
n3:cisloPeriodika
3
n3:dodaniDat
n9:2011
n3:domaciTvurceVysledku
n18:2016249 n18:4473566
n3:druhVysledku
n13:J
n3:duvernostUdaju
n8:S
n3:entitaPredkladatele
n11:predkladatel
n3:idSjednocenehoVysledku
269691
n3:idVysledku
RIV/61989592:15310/10:10211974
n3:jazykVysledku
n19:eng
n3:klicovaSlova
virusresistance; sbm-1; Pea seed-borne mosaic virus; pea; Potyvirus; marker assisted breeding; eIF4E
n3:klicoveSlovo
n5:pea n5:virusresistance n5:Potyvirus n5:eIF4E n5:Pea%20seed-borne%20mosaic%20virus n5:sbm-1 n5:marker%20assisted%20breeding
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[7A828A755971]
n3:nazevZdroje
Molecular Breeding
n3:obor
n6:EB
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
3
n3:projekt
n12:QI91A229 n12:LA08011
n3:rokUplatneniVysledku
n9:2010
n3:svazekPeriodika
26
n3:tvurceVysledku
Navrátil, Milan Šafářová, Dana Smýkal, Petr
n3:wos
000282426600005
n3:zamer
n10:MSM2678424601
s:issn
1380-3743
s:numberOfPages
14
n15:organizacniJednotka
15310