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Statements

Subject Item
n2:RIV%2F61989592%3A15310%2F09%3A00010627%21RIV10-MSM-15310___
rdf:type
skos:Concept n18:Vysledek
dcterms:description
Various experiments were conducted to characterize and control factors affecting shoot-tip necrosis (STN) in Harpagophytum procumbens. Higher cytokinin concentrations increased the incidence of STN and the problem was aggravated by the addition of auxin (IAA) to the multiplication medium. Optimum shoot multiplication was achieved by omitting auxin and using the cytokinin meta-Topolin riboside (mTR). In the presence of auxin, plantlets produced basal callus that interfered with rooting. The quantity of this basal callus was minimal when mTR was used. Increasing the concentration of either calcium or boron prevented the development of necrotic shoots. When the concentration of both elements was increased simultaneously, negative effects on both growth and STN were observed. Using 6 mM Ca in half-strength MS medium was optimum. Boron was toxic at higher (0.4 and 0.5 mM) concentrations. Plantlets rooted readily in half-strength cytokinin-free MS media supplemented with 2.5 mu M IAA. Rooted plantlets produ Various experiments were conducted to characterize and control factors affecting shoot-tip necrosis (STN) in Harpagophytum procumbens. Higher cytokinin concentrations increased the incidence of STN and the problem was aggravated by the addition of auxin (IAA) to the multiplication medium. Optimum shoot multiplication was achieved by omitting auxin and using the cytokinin meta-Topolin riboside (mTR). In the presence of auxin, plantlets produced basal callus that interfered with rooting. The quantity of this basal callus was minimal when mTR was used. Increasing the concentration of either calcium or boron prevented the development of necrotic shoots. When the concentration of both elements was increased simultaneously, negative effects on both growth and STN were observed. Using 6 mM Ca in half-strength MS medium was optimum. Boron was toxic at higher (0.4 and 0.5 mM) concentrations. Plantlets rooted readily in half-strength cytokinin-free MS media supplemented with 2.5 mu M IAA. Rooted plantlets produ
dcterms:title
Solving the problem of shoot-tip necrosis in Harpagophytum procumbens by changing the cytokinin types, calcium and boron concentrations in the medium Solving the problem of shoot-tip necrosis in Harpagophytum procumbens by changing the cytokinin types, calcium and boron concentrations in the medium
skos:prefLabel
Solving the problem of shoot-tip necrosis in Harpagophytum procumbens by changing the cytokinin types, calcium and boron concentrations in the medium Solving the problem of shoot-tip necrosis in Harpagophytum procumbens by changing the cytokinin types, calcium and boron concentrations in the medium
skos:notation
RIV/61989592:15310/09:00010627!RIV10-MSM-15310___
n3:aktivita
n8:P n8:Z
n3:aktivity
P(GA522/06/0108), Z(AV0Z50380511), Z(MSM6198959216)
n3:cisloPeriodika
1
n3:dodaniDat
n17:2010
n3:domaciTvurceVysledku
n4:5991803
n3:druhVysledku
n5:J
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
342323
n3:idVysledku
RIV/61989592:15310/09:00010627
n3:jazykVysledku
n14:eng
n3:klicovaSlova
Boron; Calcium; Cytokinins; Harpagophytum procumbens; Regeneration; Shoot-tip necrosis; Topolins
n3:klicoveSlovo
n10:Harpagophytum%20procumbens n10:Calcium n10:Cytokinins n10:Regeneration n10:Boron n10:Topolins n10:Shoot-tip%20necrosis
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[EE799200B7E1]
n3:nazevZdroje
South African Journal of Botany
n3:obor
n11:EF
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
5
n3:projekt
n9:GA522%2F06%2F0108
n3:rokUplatneniVysledku
n17:2009
n3:svazekPeriodika
75
n3:tvurceVysledku
Jain, N. Stirk, Wendy A. Van Staden, Johanes Bairu, M. W. Doležal, Karel
n3:zamer
n16:MSM6198959216 n16:AV0Z50380511
s:issn
0254-6299
s:numberOfPages
6
n13:organizacniJednotka
15310