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Statements

Subject Item
n2:RIV%2F61989592%3A15310%2F09%3A00010605%21RIV10-MSM-15310___
rdf:type
skos:Concept n10:Vysledek
dcterms:description
We investigated the effectiveness of short mitochondrial DNA fragments for the identification of lycid larvae. The rrnL, cox1 and nad5 mtDNA sequences from 17 specimens of immature stages of Lycidae and Lampyridae were combined with a previously published dataset of homologous fragments representing all major lineages of Lycidae and outgroups. Their relationships were analysed under parsimony criteria. We demonstrate that high-density profiles are necessary for accurate identification of unknown samples to generic and tribal levels and that a multilocus approach is critical for obtaining reliable results. Although widely used, the cox1 mtDNA fragment showed the worst performance for identification at genus level when the query species was not present in the library. Stronger support for deeper branches came from rrnL mtDNA. The neotenic female larvae and male adult stages of Platerodrilus sp. and Macrolibnetis depressus Pic, 1938 were associated by mtDNA fragments. Based on the present identification, We investigated the effectiveness of short mitochondrial DNA fragments for the identification of lycid larvae. The rrnL, cox1 and nad5 mtDNA sequences from 17 specimens of immature stages of Lycidae and Lampyridae were combined with a previously published dataset of homologous fragments representing all major lineages of Lycidae and outgroups. Their relationships were analysed under parsimony criteria. We demonstrate that high-density profiles are necessary for accurate identification of unknown samples to generic and tribal levels and that a multilocus approach is critical for obtaining reliable results. Although widely used, the cox1 mtDNA fragment showed the worst performance for identification at genus level when the query species was not present in the library. Stronger support for deeper branches came from rrnL mtDNA. The neotenic female larvae and male adult stages of Platerodrilus sp. and Macrolibnetis depressus Pic, 1938 were associated by mtDNA fragments. Based on the present identification,
dcterms:title
Identification of net-winged beetle larvae (Coleoptera: Lycidae) using three mtDNA fragments: a comparison of their utility Identification of net-winged beetle larvae (Coleoptera: Lycidae) using three mtDNA fragments: a comparison of their utility
skos:prefLabel
Identification of net-winged beetle larvae (Coleoptera: Lycidae) using three mtDNA fragments: a comparison of their utility Identification of net-winged beetle larvae (Coleoptera: Lycidae) using three mtDNA fragments: a comparison of their utility
skos:notation
RIV/61989592:15310/09:00010605!RIV10-MSM-15310___
n3:aktivita
n8:Z n8:P
n3:aktivity
P(GA206/06/1392), Z(MSM6198959212)
n3:cisloPeriodika
2
n3:dodaniDat
n14:2010
n3:domaciTvurceVysledku
n17:6419941 n17:4083806
n3:druhVysledku
n12:J
n3:duvernostUdaju
n9:S
n3:entitaPredkladatele
n11:predkladatel
n3:idSjednocenehoVysledku
318355
n3:idVysledku
RIV/61989592:15310/09:00010605
n3:jazykVysledku
n18:eng
n3:klicovaSlova
Coleoptera; Lycidae; larvae; mtDNA; identification
n3:klicoveSlovo
n4:Coleoptera n4:mtDNA n4:Lycidae n4:larvae n4:identification
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[60741E6BA4BB]
n3:nazevZdroje
Systematic Entomology
n3:obor
n16:EG
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
2
n3:projekt
n13:GA206%2F06%2F1392
n3:rokUplatneniVysledku
n14:2009
n3:svazekPeriodika
34
n3:tvurceVysledku
Bocák, Ladislav Levkaničová, Zuzana
n3:zamer
n6:MSM6198959212
s:issn
0307-6970
s:numberOfPages
12
n19:organizacniJednotka
15310