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Statements

Subject Item
n2:RIV%2F61989592%3A15310%2F09%3A00010411%21RIV10-MSM-15310___
rdf:type
n3:Vysledek skos:Concept
dcterms:description
The assessment of capillary electrophoresis for separation and identification of microorganisms is described in this article. The work brings a comparison of the application of uncoated capillaries modified with poly(ethylene oxide) and coated capillaries for the separation of model microorganisms Saccharomyces cerevisiae and Escherichia coli with Tris-borate-EDTA and Tris-citrate-fructose as electrolytes. The best separation was achieved in the coated capillary using 1 mM Tris-citrate-fructose buffer, pH 6.9. A simple identification based on the migration time and UV spectrum was found unsatisfactory and thus a method based on a post-separation cultivation and sequential analysis was developed. Off-line combination with mass spectrometric analysis with the use of desorption electrospray was shown to be an interesting alternative in a study of microorganisms. Mass spectra allowed distinguishing among analyzed cells. The assessment of capillary electrophoresis for separation and identification of microorganisms is described in this article. The work brings a comparison of the application of uncoated capillaries modified with poly(ethylene oxide) and coated capillaries for the separation of model microorganisms Saccharomyces cerevisiae and Escherichia coli with Tris-borate-EDTA and Tris-citrate-fructose as electrolytes. The best separation was achieved in the coated capillary using 1 mM Tris-citrate-fructose buffer, pH 6.9. A simple identification based on the migration time and UV spectrum was found unsatisfactory and thus a method based on a post-separation cultivation and sequential analysis was developed. Off-line combination with mass spectrometric analysis with the use of desorption electrospray was shown to be an interesting alternative in a study of microorganisms. Mass spectra allowed distinguishing among analyzed cells.
dcterms:title
Assessment of CE for the identification of microorganisms Assessment of CE for the identification of microorganisms
skos:prefLabel
Assessment of CE for the identification of microorganisms Assessment of CE for the identification of microorganisms
skos:notation
RIV/61989592:15310/09:00010411!RIV10-MSM-15310___
n4:aktivita
n14:S n14:Z
n4:aktivity
S, Z(MSM6198959216)
n4:cisloPeriodika
3
n4:dodaniDat
n8:2010
n4:domaciTvurceVysledku
n5:7710607 n5:2465205 n5:4316851 n5:6267408 Kowalska, Marta n5:6833446 n5:1239287 Znaleziona, Joanna n5:4536274 n5:2008998 n5:2098628
n4:druhVysledku
n13:J
n4:duvernostUdaju
n17:S
n4:entitaPredkladatele
n6:predkladatel
n4:idSjednocenehoVysledku
304181
n4:idVysledku
RIV/61989592:15310/09:00010411
n4:jazykVysledku
n12:eng
n4:klicovaSlova
Capillary electrophoresis; Desorption electrospray; Identification; Mass spectrometry; Microorganism
n4:klicoveSlovo
n11:Capillary%20electrophoresis n11:Mass%20spectrometry n11:Desorption%20electrospray n11:Identification n11:Microorganism
n4:kodStatuVydavatele
DE - Spolková republika Německo
n4:kontrolniKodProRIV
[62B87B910098]
n4:nazevZdroje
Electrophoresis
n4:obor
n18:CB
n4:pocetDomacichTvurcuVysledku
11
n4:pocetTvurcuVysledku
11
n4:rokUplatneniVysledku
n8:2009
n4:svazekPeriodika
30
n4:tvurceVysledku
Lemr, Karel Knob, Radim Petr, Jan Hinnerová, Petra Frébort, Ivo Ryparová, Olga Znaleziona, Joanna Maier, Vítězslav Ranc, Václav Kowalska, Marta Ševčík, Juraj
n4:zamer
n16:MSM6198959216
s:issn
0173-0835
s:numberOfPages
6
n15:organizacniJednotka
15310