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Statements

Subject Item
n2:RIV%2F61989592%3A15310%2F09%3A00010056%21RIV10-MSM-15310___
rdf:type
n3:Vysledek skos:Concept
dcterms:description
This paper reports on the structural rearrangement of satellite DNA type I repeats and heterochromatin during the dedifferentiation and cell cycling of mesophyll protoplasts of cucumber (Cucumis sativus). These repeats were localized in the telomeric heterochromatin of cucumber chromosomes and in the chromocenters of interphase nuclei. The dramatic reduction of heterochromatin involves decondensation of subtelomeric repeats in freshly isolated protoplasts; however, there are not a great many remarkable changes in the expression profile. In spite of that, reformation of the chromocenters, occurring 48 h after protoplast isolation, is accompanied by recondensation of satellite DNA type I; however, only partial reassembly of these repeats was revealed. In this study, FISH and a flow cytometry assay show a correlation between the partial chromocenter and the repeats reassembly, and with the reentry of cultivated protoplasts into the cell cycle and first cell division. After that, divided cells displayed a This paper reports on the structural rearrangement of satellite DNA type I repeats and heterochromatin during the dedifferentiation and cell cycling of mesophyll protoplasts of cucumber (Cucumis sativus). These repeats were localized in the telomeric heterochromatin of cucumber chromosomes and in the chromocenters of interphase nuclei. The dramatic reduction of heterochromatin involves decondensation of subtelomeric repeats in freshly isolated protoplasts; however, there are not a great many remarkable changes in the expression profile. In spite of that, reformation of the chromocenters, occurring 48 h after protoplast isolation, is accompanied by recondensation of satellite DNA type I; however, only partial reassembly of these repeats was revealed. In this study, FISH and a flow cytometry assay show a correlation between the partial chromocenter and the repeats reassembly, and with the reentry of cultivated protoplasts into the cell cycle and first cell division. After that, divided cells displayed a
dcterms:title
Chromatin Structural Rearrangement during Dedifferentiation of Protoplasts of Cucumis sativus L. Chromatin Structural Rearrangement during Dedifferentiation of Protoplasts of Cucumis sativus L.
skos:prefLabel
Chromatin Structural Rearrangement during Dedifferentiation of Protoplasts of Cucumis sativus L. Chromatin Structural Rearrangement during Dedifferentiation of Protoplasts of Cucumis sativus L.
skos:notation
RIV/61989592:15310/09:00010056!RIV10-MSM-15310___
n4:aktivita
n17:Z
n4:aktivity
Z(MSM6198959215)
n4:cisloPeriodika
4
n4:dodaniDat
n5:2010
n4:domaciTvurceVysledku
n13:8054312 n13:5930731 n13:9124918 n13:2584913 n13:6953468 n13:4021436
n4:druhVysledku
n14:J
n4:duvernostUdaju
n16:S
n4:entitaPredkladatele
n18:predkladatel
n4:idSjednocenehoVysledku
307038
n4:idVysledku
RIV/61989592:15310/09:00010056
n4:jazykVysledku
n12:eng
n4:klicovaSlova
cell cycle; chromatin; plant protoplasts; subtelomeric repeats
n4:klicoveSlovo
n6:cell%20cycle n6:plant%20protoplasts n6:subtelomeric%20repeats n6:chromatin
n4:kodStatuVydavatele
KR - Korejská republika
n4:kontrolniKodProRIV
[7116102A5279]
n4:nazevZdroje
Molecules and Cells
n4:obor
n9:EB
n4:pocetDomacichTvurcuVysledku
6
n4:pocetTvurcuVysledku
6
n4:rokUplatneniVysledku
n5:2009
n4:svazekPeriodika
27
n4:tvurceVysledku
Doležalová, Ivana Lebeda, Aleš Nádvorník, Petr Ondřej, Vladan Kitner, Miloslav Navrátilová, Božena
n4:zamer
n11:MSM6198959215
s:issn
1016-8478
s:numberOfPages
5
n15:organizacniJednotka
15310