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Statements

Subject Item
n2:RIV%2F61989592%3A15310%2F08%3A00005260%21RIV09-MZE-15310___
rdf:type
n3:Vysledek skos:Concept
dcterms:description
Embryo-rescue was used to facilitate interspecific hybridization of Cucumis anguria L. and C. zeyheri Sond. (Cucurbitaceae). Embryos were excised from developing fruits in one week intervals for six weeks after hand pollination. Various culture media were compared. Media containing coconut water were the most suitable for initial germination, and a medium with ascorbic acid provided for the best embryo development and plant recovery. Viable plants were obtained from embryos, and their hybrid character was observed by visual inspection of several morphological traits and then confirmed by isozyme analyses. These plants showed characteristics from both parents, and analysis of the leucine aminopeptidase (LAP) locus revealed three hybrid types (i.e. H1.1, H1.2 and H2). Embryo-rescue was used to facilitate interspecific hybridization of Cucumis anguria L. and C. zeyheri Sond. (Cucurbitaceae). Embryos were excised from developing fruits in one week intervals for six weeks after hand pollination. Various culture media were compared. Media containing coconut water were the most suitable for initial germination, and a medium with ascorbic acid provided for the best embryo development and plant recovery. Viable plants were obtained from embryos, and their hybrid character was observed by visual inspection of several morphological traits and then confirmed by isozyme analyses. These plants showed characteristics from both parents, and analysis of the leucine aminopeptidase (LAP) locus revealed three hybrid types (i.e. H1.1, H1.2 and H2). Metoda embryo-rescue byla využita při interspecifické hybridizaci Cucumis anguria a C. zeyheri. Embrya byla extirpována z plodů 1 - 6 týdnů po opylení. C. anguria byla použita jako mateřská komponenta, C. zeyheri jako otcovská. Pro kultivaci izolovaných embryí byla použita různá média. Média s kokosovým mlékem byla nejvhodnější pro klíčení nezralých embryí, média s kyselinou askorbovou a caseinhydrolyzátem byla vhodná pro následnou organogenezi. Byly získany rostliny, jejichž hybridní charakter byl potvrzen jak morfologicky (pomocí vybraných znaků), tak biochemicky (pomocí isozymové analýzy). Byly určeny 3 typy hybridů (H1.1., H1.2., H2.).
dcterms:title
Interspecific hybridization of Cucumis anguria L. var. longaculeatus J. H. Kirk. and C. zeyheri Sond. via embryo-rescue Interspecific hybridization of Cucumis anguria L. var. longaculeatus J. H. Kirk. and C. zeyheri Sond. via embryo-rescue Interspecifická hybridizace Cucumis anguria L. var. longaculeatus a C. zeyheri Sond. pomocí embryo-rescue
skos:prefLabel
Interspecifická hybridizace Cucumis anguria L. var. longaculeatus a C. zeyheri Sond. pomocí embryo-rescue Interspecific hybridization of Cucumis anguria L. var. longaculeatus J. H. Kirk. and C. zeyheri Sond. via embryo-rescue Interspecific hybridization of Cucumis anguria L. var. longaculeatus J. H. Kirk. and C. zeyheri Sond. via embryo-rescue
skos:notation
RIV/61989592:15310/08:00005260!RIV09-MZE-15310___
n4:aktivita
n10:Z n10:P
n4:aktivity
P(QD1357), P(QF4108), Z(MSM6198959215)
n4:cisloPeriodika
4
n4:dodaniDat
n13:2009
n4:domaciTvurceVysledku
n5:8054312 n5:1314335 n5:2780631 n5:2584913
n4:druhVysledku
n12:J
n4:duvernostUdaju
n9:S
n4:entitaPredkladatele
n17:predkladatel
n4:idSjednocenehoVysledku
373041
n4:idVysledku
RIV/61989592:15310/08:00005260
n4:jazykVysledku
n16:eng
n4:klicovaSlova
crossability; Cucumis spp.; embryo-rescue cultures; germinating capacity; isozyme analyses; morphology; regeneration ability; resistance; wild species
n4:klicoveSlovo
n6:regeneration%20ability n6:germinating%20capacity n6:crossability n6:wild%20species n6:morphology n6:embryo-rescue%20cultures n6:resistance n6:isozyme%20analyses n6:Cucumis%20spp.
n4:kodStatuVydavatele
CZ - Česká republika
n4:kontrolniKodProRIV
[2D4BA9A4CA91]
n4:nazevZdroje
Biologia Plantarum
n4:obor
n18:EF
n4:pocetDomacichTvurcuVysledku
4
n4:pocetTvurcuVysledku
5
n4:projekt
n8:QD1357 n8:QF4108
n4:rokUplatneniVysledku
n13:2008
n4:svazekPeriodika
52
n4:tvurceVysledku
Skálová, D. Navrátilová, Božena Lebeda, Aleš Křístková, Eva Dziechciarková, Marta
n4:zamer
n19:MSM6198959215
s:issn
0006-3134
s:numberOfPages
4
n14:organizacniJednotka
15310