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Statements

Subject Item
n2:RIV%2F61989592%3A15310%2F06%3A00010655%21RIV10-MSM-15310___
rdf:type
skos:Concept n15:Vysledek
dcterms:description
Background: PTEN is a tumour suppressor protein with phosphatase activity frequently altered in several types of human cancers. Materials and Methods: The PTEN effect was studied on the cell cycle (by bromdeoxyuridine incorporation) and on the phosphatidylinositol-3-kinaselprotein kinase B/Akt (PI3-K/PKB/Akt) pathway regulating proteins (by immunocytochemical, Western blot analysis and kinase assay) upon transfection of wild-type PTEN and its mutant H123Y in breast cancer cell lines. Results: The expression of the important proteins in the MCF-7 and BT-549 cells was characterised and the cellular localisation of PTEN was analysed. Transfection of H123Y led to the down-regulation of p27(KiP1) and p21(Waf1/Cip1) protein levels and the up-regulation of phosphorylated PKB/Akt. An overexpression of PTEN decreased cyclin E/cdk2 activity and inhibited S-phase entry in MCF-7. In BT-549 these changes were not observed, but overexpression of PTEN led to a diminution of PKB/Akt phosphorylation. Conclusion: PTEN Background: PTEN is a tumour suppressor protein with phosphatase activity frequently altered in several types of human cancers. Materials and Methods: The PTEN effect was studied on the cell cycle (by bromdeoxyuridine incorporation) and on the phosphatidylinositol-3-kinaselprotein kinase B/Akt (PI3-K/PKB/Akt) pathway regulating proteins (by immunocytochemical, Western blot analysis and kinase assay) upon transfection of wild-type PTEN and its mutant H123Y in breast cancer cell lines. Results: The expression of the important proteins in the MCF-7 and BT-549 cells was characterised and the cellular localisation of PTEN was analysed. Transfection of H123Y led to the down-regulation of p27(KiP1) and p21(Waf1/Cip1) protein levels and the up-regulation of phosphorylated PKB/Akt. An overexpression of PTEN decreased cyclin E/cdk2 activity and inhibited S-phase entry in MCF-7. In BT-549 these changes were not observed, but overexpression of PTEN led to a diminution of PKB/Akt phosphorylation. Conclusion: PTEN
dcterms:title
Tumour suppressor PTEN regulates cell cycle and protein kinase B/Akt pathway in breast cancer cells Tumour suppressor PTEN regulates cell cycle and protein kinase B/Akt pathway in breast cancer cells
skos:prefLabel
Tumour suppressor PTEN regulates cell cycle and protein kinase B/Akt pathway in breast cancer cells Tumour suppressor PTEN regulates cell cycle and protein kinase B/Akt pathway in breast cancer cells
skos:notation
RIV/61989592:15310/06:00010655!RIV10-MSM-15310___
n3:aktivita
n7:P n7:Z
n3:aktivity
P(GA204/01/0488), P(NC6779), P(NR7828), Z(AV0Z50380511), Z(MSM6198959216)
n3:cisloPeriodika
2A
n3:dodaniDat
n11:2010
n3:domaciTvurceVysledku
n10:2619725 n10:9961216 n10:9582320 n10:2411520 n10:4445082
n3:druhVysledku
n19:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
504532
n3:idVysledku
RIV/61989592:15310/06:00010655
n3:jazykVysledku
n13:eng
n3:klicovaSlova
breast cancer cell lines; cell cycle; phosphatase activity; PKB/Akt; PTEN; tumour suppressor; p27(Kip1); p21(Waf1/Cip1); p53 and pRb
n3:klicoveSlovo
n5:breast%20cancer%20cell%20lines n5:PKB%2FAkt n5:PTEN n5:tumour%20suppressor n5:phosphatase%20activity n5:cell%20cycle n5:p21%28Waf1%2FCip1%29 n5:p27%28Kip1%29 n5:p53%20and%20pRb
n3:kodStatuVydavatele
GR - Řecká republika
n3:kontrolniKodProRIV
[DA1BB8952A68]
n3:nazevZdroje
Anticancer Research
n3:obor
n18:FD
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
6
n3:projekt
n9:GA204%2F01%2F0488 n9:NC6779 n9:NR7828
n3:rokUplatneniVysledku
n11:2006
n3:svazekPeriodika
26
n3:tvurceVysledku
Hlobilková, Alice Šváchová, Michaela Kolář, Zdeněk Knillová, Jana Skypalová, Petra Kryštof, Vladimír
n3:zamer
n4:AV0Z50380511 n4:MSM6198959216
s:issn
0250-7005
s:numberOfPages
8
n6:organizacniJednotka
15310