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Statements

Subject Item
n2:RIV%2F61989592%3A15310%2F06%3A00005815%21RIV10-MSM-15310___
rdf:type
n7:Vysledek skos:Concept
dcterms:description
A miniaturized post-column fluorimetric detection cell for capillary separation methods based on optical fibers and liquid core waveguides (LCWs) is described. The main part of the detection cell is a fused-silica capillary coated with Teflon AF serving as an LCW. The optical fibers are used both for coupling the excitation source with the detection domain in the LCW and for the axial fluorescence collection from the LCW end. The latter fiber is connected with a compact CCD spectrometer that serves for the rejection of the scattered excitation light and for the fluorescence signal detection. The proposed design offers a compact fluorescence detector for various microcolumn separation techniques without optical elements such as filters or objectives. Moreover, its construction and optical adjustment are very simple and the whole system is highly miniaturized. The function of the detection cell is demonstrated by CE of amino acids labelled by fluorescein-based tags. Separations of different standard ami A miniaturized post-column fluorimetric detection cell for capillary separation methods based on optical fibers and liquid core waveguides (LCWs) is described. The main part of the detection cell is a fused-silica capillary coated with Teflon AF serving as an LCW. The optical fibers are used both for coupling the excitation source with the detection domain in the LCW and for the axial fluorescence collection from the LCW end. The latter fiber is connected with a compact CCD spectrometer that serves for the rejection of the scattered excitation light and for the fluorescence signal detection. The proposed design offers a compact fluorescence detector for various microcolumn separation techniques without optical elements such as filters or objectives. Moreover, its construction and optical adjustment are very simple and the whole system is highly miniaturized. The function of the detection cell is demonstrated by CE of amino acids labelled by fluorescein-based tags. Separations of different standard ami
dcterms:title
Miniaturized liquid core waveguide-based fluorimetric detection cell for capillary separation methods: Application in CE of amino acids Miniaturized liquid core waveguide-based fluorimetric detection cell for capillary separation methods: Application in CE of amino acids
skos:prefLabel
Miniaturized liquid core waveguide-based fluorimetric detection cell for capillary separation methods: Application in CE of amino acids Miniaturized liquid core waveguide-based fluorimetric detection cell for capillary separation methods: Application in CE of amino acids
skos:notation
RIV/61989592:15310/06:00005815!RIV10-MSM-15310___
n3:aktivita
n4:S n4:P n4:Z
n3:aktivity
P(GA203/06/1179), P(LC06023), S, Z(AV0Z40310501), Z(MSM6198959216)
n3:cisloPeriodika
23
n3:dodaniDat
n14:2010
n3:domaciTvurceVysledku
n9:9063455
n3:druhVysledku
n8:J
n3:duvernostUdaju
n15:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
485887
n3:idVysledku
RIV/61989592:15310/06:00005815
n3:jazykVysledku
n18:eng
n3:klicovaSlova
Amino acids; CE; Fluorescence detection; Liquid core waveguide; Separation methods
n3:klicoveSlovo
n5:Liquid%20core%20waveguide n5:CE n5:Amino%20acids n5:Separation%20methods n5:Fluorescence%20detection
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[2AD983578CB5]
n3:nazevZdroje
Electrophoresis
n3:obor
n13:CB
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
4
n3:projekt
n11:LC06023 n11:GA203%2F06%2F1179
n3:rokUplatneniVysledku
n14:2006
n3:svazekPeriodika
27
n3:tvurceVysledku
Košťál, Vratislav Zeisbergerová, Marta Šlais, Karel Kahle, Vladislav
n3:zamer
n10:MSM6198959216 n10:AV0Z40310501
s:issn
0173-0835
s:numberOfPages
8
n19:organizacniJednotka
15310