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Statements

Subject Item
n2:RIV%2F61989592%3A15310%2F06%3A00002800%21RIV07-MSM-15310___
rdf:type
n18:Vysledek skos:Concept
dcterms:description
CKX (cytokinin dehydrogenase) is a flavoprotein that cleaves cytokinins to adenine and the corresponding side-chain aldehyde using a quinone-type electron acceptor. In the present study, reactions of maize (Zea mays) CKX with five different substrates (N6-isopentenyladenine, trans-zeatin, kinetin, p-topolin and Nmethyl- isopentenyladenine) were studied. By using stopped-flow analysis of the reductive half-reaction, spectral intermediates were observed indicative of the transient formation of a binary enzyme-product complex between the cytokinin imine and the reduced enzyme. The reduction rate was high for isoprenoid cytokinins that showed formation of a charge-transfer complex of reduced enzyme with bound cytokinin imine. For the other cytokinins, flavin reduction was slow and no charge-transfer intermediates were observed. The binary complex of reduced enzyme and imine product intermediate decays relatively slowly to form an unbound product, cytokinin imine, which accumulates in the reaction mixture Byly studovány reakce kukuřičné cytokininoxidasy/dehydrogenasy s pěti různými substráty (N6-isopentenyladenin, trans-zeatin, kinetin, p-topolin and Nmethyl- isopentenyladenin). Pomocí metody zastaveného toku byly spektrofotometricky sledovány reakční meziprodukty. Byla prokázána tvorba přechodného binárního komplexu mezi redukovaným enzymem a iminovým derivátem substrátu. Stabilita iminových produktů umožnila jejich strukturní analýzu pomocí hmotnostní spektrometrie. CKX (cytokinin dehydrogenase) is a flavoprotein that cleaves cytokinins to adenine and the corresponding side-chain aldehyde using a quinone-type electron acceptor. In the present study, reactions of maize (Zea mays) CKX with five different substrates (N6-isopentenyladenine, trans-zeatin, kinetin, p-topolin and Nmethyl- isopentenyladenine) were studied. By using stopped-flow analysis of the reductive half-reaction, spectral intermediates were observed indicative of the transient formation of a binary enzyme-product complex between the cytokinin imine and the reduced enzyme. The reduction rate was high for isoprenoid cytokinins that showed formation of a charge-transfer complex of reduced enzyme with bound cytokinin imine. For the other cytokinins, flavin reduction was slow and no charge-transfer intermediates were observed. The binary complex of reduced enzyme and imine product intermediate decays relatively slowly to form an unbound product, cytokinin imine, which accumulates in the reaction mixture
dcterms:title
Kinetic and chemical analyses of the cytokinin oxidase/dehydrogenase catalysed reaction: correlations with the crystal structure Kinetické a chemické analýzy reakce katalyzované cytokininoxidasou/dehydrogenasou: korelace s krystalovou strukturou Kinetic and chemical analyses of the cytokinin oxidase/dehydrogenase catalysed reaction: correlations with the crystal structure
skos:prefLabel
Kinetické a chemické analýzy reakce katalyzované cytokininoxidasou/dehydrogenasou: korelace s krystalovou strukturou Kinetic and chemical analyses of the cytokinin oxidase/dehydrogenase catalysed reaction: correlations with the crystal structure Kinetic and chemical analyses of the cytokinin oxidase/dehydrogenase catalysed reaction: correlations with the crystal structure
skos:notation
RIV/61989592:15310/06:00002800!RIV07-MSM-15310___
n4:strany
113-124
n4:aktivita
n12:Z
n4:aktivity
Z(MSM6198959216)
n4:cisloPeriodika
Part 1
n4:dodaniDat
n6:2007
n4:domaciTvurceVysledku
n5:3343219 n5:5609763 n5:4536274 n5:3686957
n4:druhVysledku
n15:J
n4:duvernostUdaju
n10:S
n4:entitaPredkladatele
n14:predkladatel
n4:idSjednocenehoVysledku
481657
n4:idVysledku
RIV/61989592:15310/06:00002800
n4:jazykVysledku
n16:eng
n4:klicovaSlova
cytokinin; cytokinin dehydrogenase (CKX); flavoprotein; maize; mass spectrometry (MS); stopped-flow spectrophotometry
n4:klicoveSlovo
n11:stopped-flow%20spectrophotometry n11:cytokinin%20dehydrogenase%20%28CKX%29 n11:maize n11:cytokinin n11:mass%20spectrometry%20%28MS%29 n11:flavoprotein
n4:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n4:kontrolniKodProRIV
[6B3B0B4CE7CB]
n4:nazevZdroje
Biochemical Journal
n4:obor
n9:CE
n4:pocetDomacichTvurcuVysledku
4
n4:pocetTvurcuVysledku
6
n4:rokUplatneniVysledku
n6:2006
n4:svazekPeriodika
398
n4:tvurceVysledku
Fraaije, Marco W. Frébortová, Jitka Bilyeu, Krsitin D. Novák, Ondřej Popelková, Hana Frébort, Ivo
n4:zamer
n8:MSM6198959216
s:issn
0264-6021
s:numberOfPages
12
n17:organizacniJednotka
15310