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Statements

Subject Item
n2:RIV%2F61989592%3A15310%2F05%3A00010650%21RIV10-MSM-15310___
rdf:type
skos:Concept n15:Vysledek
dcterms:description
Proteolytic enzymes (peptidases) are important tools for proteomic research. This article reviews their application to digestion of proteins, which commonly precedes the mass spectrometry analysis of proteins. In the opening part, mechanism of proteolysis is described together with a detailed introduction to classification and nomenclature of enzymes. The following part brings an overview of proteolytic enzymes that are currently used in expression and functional proteomics including their properties and specificity. The review continues with reports on assays of proteolytic activity and computer prediction of peptide cleavage. The methodology of in-solution and in-gel digestion of samples and the use of peptidases for cleavage of proteins on membrane surfaces is also mentioned. Furthermore, chemical modifications of peptidases for improving their stability to autolysis and thermal inactivation are documented. Peptidase treatment of hydrophobic proteins in solutions containing organic solvents are als Proteolytic enzymes (peptidases) are important tools for proteomic research. This article reviews their application to digestion of proteins, which commonly precedes the mass spectrometry analysis of proteins. In the opening part, mechanism of proteolysis is described together with a detailed introduction to classification and nomenclature of enzymes. The following part brings an overview of proteolytic enzymes that are currently used in expression and functional proteomics including their properties and specificity. The review continues with reports on assays of proteolytic activity and computer prediction of peptide cleavage. The methodology of in-solution and in-gel digestion of samples and the use of peptidases for cleavage of proteins on membrane surfaces is also mentioned. Furthermore, chemical modifications of peptidases for improving their stability to autolysis and thermal inactivation are documented. Peptidase treatment of hydrophobic proteins in solutions containing organic solvents are als
dcterms:title
Proteolytic enzymes: Significance for proteomics Proteolytic enzymes: Significance for proteomics
skos:prefLabel
Proteolytic enzymes: Significance for proteomics Proteolytic enzymes: Significance for proteomics
skos:notation
RIV/61989592:15310/05:00010650!RIV10-MSM-15310___
n3:aktivita
n8:Z
n3:aktivity
Z(MSM6198959216)
n3:cisloPeriodika
12
n3:dodaniDat
n10:2010
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n17:J
n3:duvernostUdaju
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n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
539384
n3:idVysledku
RIV/61989592:15310/05:00010650
n3:jazykVysledku
n18:eng
n3:klicovaSlova
tandem mass-spectrometry; amino-acid sequence; protein identification; polyacrylamide-gels; quadrupole-time; lysobacter-enzymogenes; chemical-modification; carboxypeptidase-Y; pseudomonas-fragi; membrane-proteins
n3:klicoveSlovo
n4:quadrupole-time n4:amino-acid%20sequence n4:chemical-modification n4:protein%20identification n4:pseudomonas-fragi n4:carboxypeptidase-Y n4:membrane-proteins n4:polyacrylamide-gels n4:tandem%20mass-spectrometry n4:lysobacter-enzymogenes
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[B1E78F703D8B]
n3:nazevZdroje
Chemické listy
n3:obor
n14:EB
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
4
n3:rokUplatneniVysledku
n10:2005
n3:svazekPeriodika
99
n3:tvurceVysledku
Lenobel, René Šebela, Marek Štosová, Taťána Havliś, Jan
n3:zamer
n12:MSM6198959216
s:issn
0009-2770
s:numberOfPages
10
n13:organizacniJednotka
15310