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Statements

Subject Item
n2:RIV%2F61989592%3A15310%2F05%3A00009862%21RIV10-MSM-15310___
rdf:type
skos:Concept n13:Vysledek
dcterms:description
Cytokinin metabolism in plants is very complex. More than 20 cytokinins bearing isoprenoid and aromatic side chains were identified by high performance liquid chromatography-mass spectrometry (HPLC-MS) in pea ( Pisum sativum L. cv. Gotik) leaves, indicating diverse metabolic conversions of primary products of cytokinin biosynthesis. To determine the potential involvement of two enzymes metabolizing cytokinins, cytokinin oxidase/dehydrogenase (CKX, EC 1.5.99.12) and zeatin reductase (ZRED, EC 1.3.1.69), in the control of endogenous cytokinin levels, their in vitro activities were investigated in relation to the uptake and metabolism of [2-H-3] trans- zeatin ([2-H-3] Z) in shoot explants of pea. Trans-zeatin 9-riboside, trans-zeatin 9-riboside-5'-monophosphate and cytokinin degradation products adenine and adenosine were detected as predominant [2-H-3] Z metabolites during 2, 5, 8, and 24 h incubation. Increasing formation of adenine and adenosine indicated extensive degradation of [2-H-3] Z by CKX Cytokinin metabolism in plants is very complex. More than 20 cytokinins bearing isoprenoid and aromatic side chains were identified by high performance liquid chromatography-mass spectrometry (HPLC-MS) in pea ( Pisum sativum L. cv. Gotik) leaves, indicating diverse metabolic conversions of primary products of cytokinin biosynthesis. To determine the potential involvement of two enzymes metabolizing cytokinins, cytokinin oxidase/dehydrogenase (CKX, EC 1.5.99.12) and zeatin reductase (ZRED, EC 1.3.1.69), in the control of endogenous cytokinin levels, their in vitro activities were investigated in relation to the uptake and metabolism of [2-H-3] trans- zeatin ([2-H-3] Z) in shoot explants of pea. Trans-zeatin 9-riboside, trans-zeatin 9-riboside-5'-monophosphate and cytokinin degradation products adenine and adenosine were detected as predominant [2-H-3] Z metabolites during 2, 5, 8, and 24 h incubation. Increasing formation of adenine and adenosine indicated extensive degradation of [2-H-3] Z by CKX
dcterms:title
The involvement of cytokinin oxidase/dehydrogenase and zeatin reductase in regulation of cytokinin levels in pea (Pisum sativum L.) leaves The involvement of cytokinin oxidase/dehydrogenase and zeatin reductase in regulation of cytokinin levels in pea (Pisum sativum L.) leaves
skos:prefLabel
The involvement of cytokinin oxidase/dehydrogenase and zeatin reductase in regulation of cytokinin levels in pea (Pisum sativum L.) leaves The involvement of cytokinin oxidase/dehydrogenase and zeatin reductase in regulation of cytokinin levels in pea (Pisum sativum L.) leaves
skos:notation
RIV/61989592:15310/05:00009862!RIV10-MSM-15310___
n3:aktivita
n12:S n12:P n12:Z
n3:aktivity
P(GA206/03/0313), S, Z(AV0Z50380511), Z(MSM6198959216)
n3:cisloPeriodika
3
n3:dodaniDat
n11:2010
n3:domaciTvurceVysledku
n7:6401309 n7:4712048 n7:3686957
n3:druhVysledku
n16:J
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n14:predkladatel
n3:idSjednocenehoVysledku
525647
n3:idVysledku
RIV/61989592:15310/05:00009862
n3:jazykVysledku
n9:eng
n3:klicovaSlova
Aromatic cytokinin; cis-zeatin; cytokinin; cytokinin oxidase/dehydrogenace; dihydrozeatin; metabolism; pea; trans-zeatin; zeatin reductase
n3:klicoveSlovo
n4:cis-zeatin n4:Aromatic%20cytokinin n4:dihydrozeatin n4:trans-zeatin n4:pea n4:cytokinin n4:cytokinin%20oxidase%2Fdehydrogenace n4:zeatin%20reductase n4:metabolism
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[0F44C6F3A08A]
n3:nazevZdroje
Journal of Plant Growth Regulation
n3:obor
n15:EF
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
7
n3:projekt
n19:GA206%2F03%2F0313
n3:rokUplatneniVysledku
n11:2005
n3:svazekPeriodika
24
n3:tvurceVysledku
Strnad, Miroslav Šolcová, Blanka Motyka, Václav Dobrev, Petre I. Novák, Ondřej Gaudinová, Alena Friedecký, David
n3:zamer
n17:MSM6198959216 n17:AV0Z50380511
s:issn
0721-7595
s:numberOfPages
13
n6:organizacniJednotka
15310