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Statements

Subject Item
n2:RIV%2F61989592%3A15310%2F05%3A00002191%21RIV06-MSM-15310___
rdf:type
skos:Concept n16:Vysledek
dcterms:description
The amino acid sequence of methylamine oxidase (MeAO) from the fungus Aspergillus niger was analyzed using mass spectrometry (MS). First, MeAO was characterized by an accurate molecular mass of 72.4 kDa of the monomer measured using MALDI-TOF-MS and by a pI value of 5.8 determined by isoelectric focusing. MALDI-TOF-MS revealed a clear peptide mass fingerprint after tryptic digestion, which did not provide any relevant hit when searched against a nonredundant protein database and was different from that of A. niger amine oxidase AO-I. Tandem mass spectrometry with electrospray ionization coupled to liquid chromatography allowed unambiguous reading of six peptide sequences (11-19 amino acids) and seven sequence tags (4-15 amino acids), which were used for MS BLAST homology searching. MeAO was found to be largely homologous to a hypothetical protein AN7641.2 (EMBL/GenBank protein accession code EAA61827) from Aspergillus nidulans FGSC A4 with a theoretical molecular mass of 76 460 Da and pI 6.14, which b The amino acid sequence of methylamine oxidase (MeAO) from the fungus Aspergillus niger was analyzed using mass spectrometry (MS). First, MeAO was characterized by an accurate molecular mass of 72.4 kDa of the monomer measured using MALDI-TOF-MS and by a pI value of 5.8 determined by isoelectric focusing. MALDI-TOF-MS revealed a clear peptide mass fingerprint after tryptic digestion, which did not provide any relevant hit when searched against a nonredundant protein database and was different from that of A. niger amine oxidase AO-I. Tandem mass spectrometry with electrospray ionization coupled to liquid chromatography allowed unambiguous reading of six peptide sequences (11-19 amino acids) and seven sequence tags (4-15 amino acids), which were used for MS BLAST homology searching. MeAO was found to be largely homologous to a hypothetical protein AN7641.2 (EMBL/GenBank protein accession code EAA61827) from Aspergillus nidulans FGSC A4 with a theoretical molecular mass of 76 460 Da and pI 6.14, which b Aminokyselinová sekvence methylaminoxidasy izolované z plísně Aspergillus niger byla analyzována různými MS technikami. MALDI-MS umožnila stanovení přesné hmoty monomeru o velikosti 72,4 kDa. Isoelektrický bod byl určen pomocí isoelektrické fokusace. Peptidové mapování pomocí MALDI-MS ukázalo, že sekvence methylaminoxidasy se výrazně liší od sekvence aminoxidasy AO-I. Částečná sekvence vyizolované methylaminoxidasy byla určena pomocí konbinace přístupu MALDI-PSD-MS a nanoLC-ESI-MSMS a s využitím MS BLAST softwaru pro hledání homologie podle zadaných peptidových sekvencí proteinu. V databázi byl nalezen homologní hypotetický protein AN7641.2 (EMBL/GenBank protein-accession code EAA61827) z Aspergillus nidulans FGSC A4 o teoretické molekulové hmotnosti 76.46 kDa a pI 6.14, ktery patří do skupiny aminoxidas obsahujících měď. Tento protein vykazuje pouze malou homologii k aminooxidase AO-I (32 % identity, 49 % podobnost).
dcterms:title
Mapping the primary structure of copper/topaquinone-containing methylamine oxidase from Aspergillus niger Mapování primární struktury methylaminoxidasy z Aspergillus niger obsahující topachinon a měď Mapping the primary structure of copper/topaquinone-containing methylamine oxidase from Aspergillus niger
skos:prefLabel
Mapování primární struktury methylaminoxidasy z Aspergillus niger obsahující topachinon a měď Mapping the primary structure of copper/topaquinone-containing methylamine oxidase from Aspergillus niger Mapping the primary structure of copper/topaquinone-containing methylamine oxidase from Aspergillus niger
skos:notation
RIV/61989592:15310/05:00002191!RIV06-MSM-15310___
n3:strany
401-408
n3:aktivita
n5:P n5:Z
n3:aktivity
P(ME 664), Z(MSM6198959216)
n3:cisloPeriodika
5
n3:dodaniDat
n11:2006
n3:domaciTvurceVysledku
n14:9708790 n14:4536274 n14:9896821
n3:druhVysledku
n10:J
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n13:predkladatel
n3:idSjednocenehoVysledku
528868
n3:idVysledku
RIV/61989592:15310/05:00002191
n3:jazykVysledku
n9:eng
n3:klicovaSlova
Aspergillus niger; mass specrometry; methylamine oxidase; peptide; primary structure
n3:klicoveSlovo
n8:mass%20specrometry n8:methylamine%20oxidase n8:primary%20structure n8:peptide n8:Aspergillus%20niger
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[53EF209E25FB]
n3:nazevZdroje
Folia Microbiologica
n3:obor
n19:CE
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:projekt
n4:ME%20664
n3:rokUplatneniVysledku
n11:2005
n3:svazekPeriodika
50
n3:tvurceVysledku
Frébort, Ivo Lenobel, René Šebela, Marek
n3:zamer
n7:MSM6198959216
s:issn
0015-5632
s:numberOfPages
8
n17:organizacniJednotka
15310