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Statements

Subject Item
n2:RIV%2F61989592%3A15310%2F04%3A00002446%21RIV%2F2005%2FGA0%2F153105%2FN
rdf:type
n13:Vysledek skos:Concept
dcterms:description
Jsou uvedeny nové metohologické přístupy v analýzách rostlinných hormonů. New approach in the analytical technologies of plant hormone analysis was introduced in the field of cytokinins and might be also soon applied in the analysis of other plant hormone groups. A new sensitive method for the quantitative analysis of all natural isoprenoid and aromatic cytokinins in plants is based on combined %22generic%22 immunoaffinity chromatography - electrospray single-quadrupole mass. A baseline chromatographic separation of 20 non-derivatized naturally occurring cytokinins has been obtained. Precise analysis of O-glucoside and nucleotide fractions were also performed by the HPLC-MS runs but after an enzymatic treatment. More than 50 different cytokinin metabolites can be measured by this approach. When low cone voltage (20 eV) was applied, all studied cytokinins were determined in aqueous methanol as dominant quasi-molecular ions of M+H + with limits of detection from 10 to 50 fmol. For routine analysis we obtained a linearity range between 25 (75) fmol - 100 pmol. Developed LC-MS New approach in the analytical technologies of plant hormone analysis was introduced in the field of cytokinins and might be also soon applied in the analysis of other plant hormone groups. A new sensitive method for the quantitative analysis of all natural isoprenoid and aromatic cytokinins in plants is based on combined %22generic%22 immunoaffinity chromatography - electrospray single-quadrupole mass. A baseline chromatographic separation of 20 non-derivatized naturally occurring cytokinins has been obtained. Precise analysis of O-glucoside and nucleotide fractions were also performed by the HPLC-MS runs but after an enzymatic treatment. More than 50 different cytokinin metabolites can be measured by this approach. When low cone voltage (20 eV) was applied, all studied cytokinins were determined in aqueous methanol as dominant quasi-molecular ions of M+H + with limits of detection from 10 to 50 fmol. For routine analysis we obtained a linearity range between 25 (75) fmol - 100 pmol. Developed LC-MS
dcterms:title
New methodological approaches to plant hormone analysis Nové metohologické přístupy v analýzách rostlinných hormonů New methodological approaches to plant hormone analysis
skos:prefLabel
Nové metohologické přístupy v analýzách rostlinných hormonů New methodological approaches to plant hormone analysis New methodological approaches to plant hormone analysis
skos:notation
RIV/61989592:15310/04:00002446!RIV/2005/GA0/153105/N
n3:strany
87
n3:aktivita
n14:P
n3:aktivity
P(GA522/01/0275)
n3:dodaniDat
n9:2005
n3:domaciTvurceVysledku
n7:9896821 n7:3686957 n7:4712048 n7:5991803
n3:druhVysledku
n8:D
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
576122
n3:idVysledku
RIV/61989592:15310/04:00002446
n3:jazykVysledku
n10:eng
n3:klicovaSlova
immunoaffinity chromatography;mass spectrometry;cytokinin;plant hormones
n3:klicoveSlovo
n4:cytokinin n4:immunoaffinity%20chromatography n4:mass%20spectrometry n4:plant%20hormones
n3:kontrolniKodProRIV
[D8D318B9C36A]
n3:mistoVydani
Canberra
n3:nazevZdroje
18th IPGSA conference, Conference Handbook
n3:obor
n19:EF
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
5
n3:projekt
n15:GA522%2F01%2F0275
n3:rokUplatneniVysledku
n9:2004
n3:tvurceVysledku
Doležal, Karel Novák, Ondřej Spíchal, Lukáš Lenobel, René Strnad, Miroslav
s:numberOfPages
144
n12:hasPublisher
Australian National University
n16:organizacniJednotka
15310