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Statements

Subject Item
n2:RIV%2F61989592%3A15310%2F04%3A00002282%21RIV%2F2005%2FGA0%2F153105%2FN
rdf:type
n11:Vysledek skos:Concept
dcterms:description
Measurement of the chlorophyll (Chl) a fluorescence rise (FR) under higher exciting irradiance (El), the O-J-I-P transient, or under lower irradiance, the O-I-P transient, is a routinely used method to access photosystem 2 function in thylakoid membranes of chloroplasts. Our measurements with a suspension of pea thylakoid membranes showed that the relative heights of the J and I steps in the FR depended not only on El but also on the concentration and thickness of the sample. We explain this effect as a consequence of the gradient of El within the sample. We tested this suggestion by theoretical simulations of the FR based on the model that was previously used for simulation of the FR considering in addition the gradient of El within the sample. Our theoretical results correspond well with the experiments. The irradiance gradient effect may influence measured FR significantly and this fact should be taken into consideration in the interpretation of measured FRs. Measurement of the chlorophyll (Chl) a fluorescence rise (FR) under higher exciting irradiance (El), the O-J-I-P transient, or under lower irradiance, the O-I-P transient, is a routinely used method to access photosystem 2 function in thylakoid membranes of chloroplasts. Our measurements with a suspension of pea thylakoid membranes showed that the relative heights of the J and I steps in the FR depended not only on El but also on the concentration and thickness of the sample. We explain this effect as a consequence of the gradient of El within the sample. We tested this suggestion by theoretical simulations of the FR based on the model that was previously used for simulation of the FR considering in addition the gradient of El within the sample. Our theoretical results correspond well with the experiments. The irradiance gradient effect may influence measured FR significantly and this fact should be taken into consideration in the interpretation of measured FRs. Byl studován gradient excitovaného záření bez ovlivnění vzorku relativní výškou kroku rychlosti vzrůstu fluorescence ve chlorofylu.
dcterms:title
The gradient of exciting radiation within a sample affects the relative height of steps in the fast chlorophyll a fluorescence rise Gradient excitovaného záření bez ovlivnění vzorku relativní výškou kroku rychlosti vzrůstu fluorescence ve chlorofylu The gradient of exciting radiation within a sample affects the relative height of steps in the fast chlorophyll a fluorescence rise
skos:prefLabel
Gradient excitovaného záření bez ovlivnění vzorku relativní výškou kroku rychlosti vzrůstu fluorescence ve chlorofylu The gradient of exciting radiation within a sample affects the relative height of steps in the fast chlorophyll a fluorescence rise The gradient of exciting radiation within a sample affects the relative height of steps in the fast chlorophyll a fluorescence rise
skos:notation
RIV/61989592:15310/04:00002282!RIV/2005/GA0/153105/N
n3:strany
161-172
n3:aktivita
n5:P n5:Z
n3:aktivity
P(GP204/02/P071), Z(MSM 153100010)
n3:cisloPeriodika
2
n3:dodaniDat
n9:2005
n3:domaciTvurceVysledku
n14:1274899 n14:2950561 n14:3124789
n3:druhVysledku
n7:J
n3:duvernostUdaju
n10:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
565703
n3:idVysledku
RIV/61989592:15310/04:00002282
n3:jazykVysledku
n18:eng
n3:klicovaSlova
exciting irradiance gradient;model;O-J-I-P transient;optical properties;Pisum
n3:klicoveSlovo
n6:optical%20properties n6:model n6:exciting%20irradiance%20gradient n6:O-J-I-P%20transient n6:Pisum
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[6C89C923EC55]
n3:nazevZdroje
Photosynthetica
n3:obor
n19:BO
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
5
n3:projekt
n8:GP204%2F02%2FP071
n3:rokUplatneniVysledku
n9:2004
n3:svazekPeriodika
42
n3:tvurceVysledku
Tomek, P. Lazár, Dušan Nauš, Jan Ilík, Petr Sušila, P.
n3:zamer
n16:MSM%20153100010
s:issn
0300-3604
s:numberOfPages
12
n13:organizacniJednotka
15310