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Statements

Subject Item
n2:RIV%2F61989592%3A15310%2F04%3A00002217%21RIV%2F2005%2FGA0%2F153105%2FN
rdf:type
n14:Vysledek skos:Concept
dcterms:description
Combination of high performance liquid chromatography (HPLC) or capillary liquid chromatography (CapLC) with quadrupole-time of flight mass spectrometry (Q-TOF) makes possible a new direction in plant hormone research. The instrument facilities are a good alternative for search of new plant metabolites and metabolic pathways. The recently developed method for quantitative analysis of natural cytokinins (Novák et al., 2003) was adapted from single-quadrupole mass spectrometer (SQ-MS) to Q-TOF micro instrument. The full scan spectra at very low concentration levels (100 fmol) were obtained together with MS-MS analyses of selected ions. The accurate masses of the parent ions and their fragments were calculated and used for determination of elementary structure with fidelity 10 ppm and better with a external calibration. The optimal conditions for combination of HPLC and CapLC with Q-TOF, i.e. capillary and cone voltage, desolvation gas flow and temperature for effective ionization and collision energy fo Combination of high performance liquid chromatography (HPLC) or capillary liquid chromatography (CapLC) with quadrupole-time of flight mass spectrometry (Q-TOF) makes possible a new direction in plant hormone research. The instrument facilities are a good alternative for search of new plant metabolites and metabolic pathways. The recently developed method for quantitative analysis of natural cytokinins (Novák et al., 2003) was adapted from single-quadrupole mass spectrometer (SQ-MS) to Q-TOF micro instrument. The full scan spectra at very low concentration levels (100 fmol) were obtained together with MS-MS analyses of selected ions. The accurate masses of the parent ions and their fragments were calculated and used for determination of elementary structure with fidelity 10 ppm and better with a external calibration. The optimal conditions for combination of HPLC and CapLC with Q-TOF, i.e. capillary and cone voltage, desolvation gas flow and temperature for effective ionization and collision energy fo Kombinace HPLC nebo kapilární kapalinové chromatografie (CapLC) s Q-TOF dává nové možnosti ve výzkumu rostlinných hormonů.
dcterms:title
Application of Q-TOF technology in plant hormone research Použití Q-TOF technologie při studiu rostlinných hormonů Application of Q-TOF technology in plant hormone research
skos:prefLabel
Application of Q-TOF technology in plant hormone research Použití Q-TOF technologie při studiu rostlinných hormonů Application of Q-TOF technology in plant hormone research
skos:notation
RIV/61989592:15310/04:00002217!RIV/2005/GA0/153105/N
n3:strany
124
n3:aktivita
n15:P n15:Z
n3:aktivity
P(GA522/03/0323), Z(MSM 153100008)
n3:dodaniDat
n7:2005
n3:domaciTvurceVysledku
n9:3686957 n9:9896821 n9:4712048
n3:druhVysledku
n13:D
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
555158
n3:idVysledku
RIV/61989592:15310/04:00002217
n3:jazykVysledku
n11:eng
n3:klicovaSlova
liquid chromatography;mass spectrometry;quadrupole-time of flight mass spectrometer;cytokinins;identification;quantification;exact mass determination
n3:klicoveSlovo
n6:cytokinins n6:quadrupole-time%20of%20flight%20mass%20spectrometer n6:identification n6:mass%20spectrometry n6:exact%20mass%20determination n6:liquid%20chromatography n6:quantification
n3:kontrolniKodProRIV
[84106E3138A2]
n3:mistoVydani
Canberra
n3:nazevZdroje
18th IPGSA conference, Conference Handbook
n3:obor
n17:EF
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
4
n3:projekt
n20:GA522%2F03%2F0323
n3:rokUplatneniVysledku
n7:2004
n3:tvurceVysledku
Novák, Ondřej Richer, Stephanie Strnad, Miroslav Lenobel, René
n3:zamer
n19:MSM%20153100008
s:numberOfPages
144
n8:hasPublisher
Australian National University
n12:organizacniJednotka
15310