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Statements

Subject Item
n2:RIV%2F61989592%3A15310%2F04%3A00002115%21RIV%2F2005%2FMSM%2F153105%2FN
rdf:type
skos:Concept n16:Vysledek
dcterms:description
The analytical performances of a manual and a partially automated chemiluminescent (CL) assay, of total antioxidant capacity (TAC) were assessed. In both cases the light emitting reaction involved luminol, horseradish peroxidase and hydrogen peroxyde, but the emission kinetics and the parameters taken into account to calculate TAC values were completely different. The major characteristics expressing the quality of the two analytical methods, i.e. inaccuracy, repeteability and reproducibility, sensitivity, time required for the analysis and detection limit, were estimated by using standard solutions of Trolox. The reliability of the automated method, in comparison with the more validated manual one, was demonstrated testing food samples such as honey, wine and dietary supplements and performing a statistical analysis of the results. The comparison of the two series of data by t-test resulted in p values in the range 0.1-0.01. The time required for the analysis of each sample was reduced to one third u Práce se zabývá automatizovaným a manuálním luminescenčním stanovením antioxidační kapacity. Jsou srovnány analytické rysy. The analytical performances of a manual and a partially automated chemiluminescent (CL) assay, of total antioxidant capacity (TAC) were assessed. In both cases the light emitting reaction involved luminol, horseradish peroxidase and hydrogen peroxyde, but the emission kinetics and the parameters taken into account to calculate TAC values were completely different. The major characteristics expressing the quality of the two analytical methods, i.e. inaccuracy, repeteability and reproducibility, sensitivity, time required for the analysis and detection limit, were estimated by using standard solutions of Trolox. The reliability of the automated method, in comparison with the more validated manual one, was demonstrated testing food samples such as honey, wine and dietary supplements and performing a statistical analysis of the results. The comparison of the two series of data by t-test resulted in p values in the range 0.1-0.01. The time required for the analysis of each sample was reduced to one third u
dcterms:title
Automated and manual luminescent assay of antioxidant capacity: analytical features by comparison Automated and manual luminescent assay of antioxidant capacity: analytical features by comparison Automatizované a manuální luminescenční stanovení antioxidační kapacity: srovnání analytických rysů
skos:prefLabel
Automated and manual luminescent assay of antioxidant capacity: analytical features by comparison Automated and manual luminescent assay of antioxidant capacity: analytical features by comparison Automatizované a manuální luminescenční stanovení antioxidační kapacity: srovnání analytických rysů
skos:notation
RIV/61989592:15310/04:00002115!RIV/2005/MSM/153105/N
n3:strany
665-670
n3:aktivita
n5:Z
n3:aktivity
Z(MSM 153100007)
n3:cisloPeriodika
3
n3:dodaniDat
n17:2005
n3:domaciTvurceVysledku
n18:5136326
n3:druhVysledku
n11:J
n3:duvernostUdaju
n7:S
n3:entitaPredkladatele
n12:predkladatel
n3:idSjednocenehoVysledku
555644
n3:idVysledku
RIV/61989592:15310/04:00002115
n3:jazykVysledku
n13:eng
n3:klicovaSlova
luminescence;antioxidant capacity;microplate luminometer;foods
n3:klicoveSlovo
n4:antioxidant%20capacity n4:foods n4:luminescence n4:microplate%20luminometer
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[45D620CE7874]
n3:nazevZdroje
Talanta
n3:obor
n6:CF
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
7
n3:rokUplatneniVysledku
n17:2004
n3:svazekPeriodika
64
n3:tvurceVysledku
Sabatini, A. G. Fini, F. Ferri, E. Bolelli, L. Sichertová, Dana Girotti, S. Budini, R.
n3:zamer
n15:MSM%20153100007
s:issn
0039-9140
s:numberOfPages
6
n14:organizacniJednotka
15310