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Statements

Subject Item
n2:RIV%2F61989592%3A15110%2F14%3A33151444%21RIV15-MSM-15110___
rdf:type
skos:Concept n16:Vysledek
dcterms:description
Objectives: Little is known about the effect of adipokines in the pathogenesis of the acute myocardial infarction (AMI) in young patients. The aim of this pilot study was to evaluate whether it is possible to detect mRNA expressions of selected adipokines in the peripheral blood leukocytes of young patients with myocardial infarction. Methods: We prospectively examined 42 patients who had an acute myocardial infarction (AMI) at a young age (men 18-45 years of age and women 18-55 years of age) and control groups of 31 patients with metabolic syndrome and 15 healthy individuals. RNA was isolated from their peripheral blood leukocytes and transcribed into cDNA (transcription kit iSCRIPT). Subsequently, the expression of the following genes were analysed (LC 480II, BioVendor kits): Adipocyte Fatty Acid Binding protein (AFABP, FABP4), Leptin and Adiponectin receptor 2 (ADN-R2). Beta-2-microglobulin was examined as a housekeeping gene. Results: In the group of patients after AMI the expression of ADN-R2 was detected in 80% patients, Leptin in 75% and FABP4 expression in 67.5%. In the control group AND-R2 and FABP4 gene were expressed in all subjects and Leptin expression was absent in 2 healthy subjects (4.3%). A correlation could not be found between FABP4 gene expression and the concentration of its product (AFABP) in the serum of the group with AMI. Conclusion: We have shown that the mRNA expression of selected adipokines can be quantified in the peripheral blood leukocytes. Whether low expressions of protective adipokines contribute to early manifestations of acute myocardial infarction needs to be explored in further studies. Objectives: Little is known about the effect of adipokines in the pathogenesis of the acute myocardial infarction (AMI) in young patients. The aim of this pilot study was to evaluate whether it is possible to detect mRNA expressions of selected adipokines in the peripheral blood leukocytes of young patients with myocardial infarction. Methods: We prospectively examined 42 patients who had an acute myocardial infarction (AMI) at a young age (men 18-45 years of age and women 18-55 years of age) and control groups of 31 patients with metabolic syndrome and 15 healthy individuals. RNA was isolated from their peripheral blood leukocytes and transcribed into cDNA (transcription kit iSCRIPT). Subsequently, the expression of the following genes were analysed (LC 480II, BioVendor kits): Adipocyte Fatty Acid Binding protein (AFABP, FABP4), Leptin and Adiponectin receptor 2 (ADN-R2). Beta-2-microglobulin was examined as a housekeeping gene. Results: In the group of patients after AMI the expression of ADN-R2 was detected in 80% patients, Leptin in 75% and FABP4 expression in 67.5%. In the control group AND-R2 and FABP4 gene were expressed in all subjects and Leptin expression was absent in 2 healthy subjects (4.3%). A correlation could not be found between FABP4 gene expression and the concentration of its product (AFABP) in the serum of the group with AMI. Conclusion: We have shown that the mRNA expression of selected adipokines can be quantified in the peripheral blood leukocytes. Whether low expressions of protective adipokines contribute to early manifestations of acute myocardial infarction needs to be explored in further studies.
dcterms:title
Determination of adipokine expression in the peripheral blood leukocytes of young patients with myocardial infarction Determination of adipokine expression in the peripheral blood leukocytes of young patients with myocardial infarction
skos:prefLabel
Determination of adipokine expression in the peripheral blood leukocytes of young patients with myocardial infarction Determination of adipokine expression in the peripheral blood leukocytes of young patients with myocardial infarction
skos:notation
RIV/61989592:15110/14:33151444!RIV15-MSM-15110___
n3:aktivita
n13:O
n3:aktivity
O
n3:cisloPeriodika
1
n3:dodaniDat
n15:2015
n3:domaciTvurceVysledku
n8:2348411 n8:4157311
n3:druhVysledku
n11:J
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n14:predkladatel
n3:idSjednocenehoVysledku
10730
n3:idVysledku
RIV/61989592:15110/14:33151444
n3:jazykVysledku
n7:eng
n3:klicovaSlova
metabolic syndrome; acute myocardial infarction; peripheral blood leukocytes; mRNA; adipokine expression; adipokines
n3:klicoveSlovo
n5:metabolic%20syndrome n5:acute%20myocardial%20infarction n5:adipokines n5:peripheral%20blood%20leukocytes n5:adipokine%20expression n5:mRNA
n3:kodStatuVydavatele
CH - Švýcarská konfederace
n3:kontrolniKodProRIV
[3095915C2980]
n3:nazevZdroje
Experimental and Clinical Cardiology
n3:obor
n9:FA
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
10
n3:rokUplatneniVysledku
n15:2014
n3:svazekPeriodika
20
n3:tvurceVysledku
Švesták, Marek Stejskal, David Cibulková, Petra Václavík, Jan Louis, Albert Janýšková, Hana Richterová, Radmila Smékal, Aleš Hutyra, Martin Táborský, Miloš
s:issn
1205-6626
s:numberOfPages
13
n10:organizacniJednotka
15110