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Statements

Subject Item
n2:RIV%2F61989592%3A15110%2F14%3A33148910%21RIV15-MSM-15110___
rdf:type
n16:Vysledek skos:Concept
rdfs:seeAlso
http://dx.doi.org/10.1016/j.electacta.2013.06.115
dcterms:description
This study focuses on application of room temperature ionic liquids (RTILs) as solubilizers, adsorption solvents and supporting electrolytes for electrochemical analysis of human and bovine serum albumins, HSA and BSA. The proteins were analyzed by ex situ, adsorptive transfer, square-wave voltammetry (SWV) at a basal-plane pyrolytic graphite electrode after solubilization using imidazolium- and ammonium-based RTILs. The application of RTILs enabled SWV scan from 0 to +1.5 V (vs. Ag/AgCl/3 M KCl) without interference with the anodic response of the proteins. Concretely, Tyr (Y) and Trp (W) oxidation currentsof HSA and BSA, peak Y&W around +0.85 V, were observed and characterized under different RTILs and RTIL/water conditions. The electrochemical data were supported by electrophoresis under denaturing and native conditions. These provided evidence for the structural changes and stability of the studied proteinsin the presence of RTILs. The data acquired using BSA and HSA model proteins, could be used in further applications of RTILs in protein electrochemistry and for developing new protein sensing strategies. This study focuses on application of room temperature ionic liquids (RTILs) as solubilizers, adsorption solvents and supporting electrolytes for electrochemical analysis of human and bovine serum albumins, HSA and BSA. The proteins were analyzed by ex situ, adsorptive transfer, square-wave voltammetry (SWV) at a basal-plane pyrolytic graphite electrode after solubilization using imidazolium- and ammonium-based RTILs. The application of RTILs enabled SWV scan from 0 to +1.5 V (vs. Ag/AgCl/3 M KCl) without interference with the anodic response of the proteins. Concretely, Tyr (Y) and Trp (W) oxidation currentsof HSA and BSA, peak Y&W around +0.85 V, were observed and characterized under different RTILs and RTIL/water conditions. The electrochemical data were supported by electrophoresis under denaturing and native conditions. These provided evidence for the structural changes and stability of the studied proteinsin the presence of RTILs. The data acquired using BSA and HSA model proteins, could be used in further applications of RTILs in protein electrochemistry and for developing new protein sensing strategies.
dcterms:title
Electrochemical oxidation of proteins using ionic liquids as solubilizers, adsorption solvents and electrolytes Electrochemical oxidation of proteins using ionic liquids as solubilizers, adsorption solvents and electrolytes
skos:prefLabel
Electrochemical oxidation of proteins using ionic liquids as solubilizers, adsorption solvents and electrolytes Electrochemical oxidation of proteins using ionic liquids as solubilizers, adsorption solvents and electrolytes
skos:notation
RIV/61989592:15110/14:33148910!RIV15-MSM-15110___
n5:aktivita
n11:I n11:P
n5:aktivity
I, P(EE2.3.20.0057)
n5:cisloPeriodika
April
n5:dodaniDat
n6:2015
n5:domaciTvurceVysledku
n7:4629868 n7:7668872 n7:4186559
n5:druhVysledku
n10:J
n5:duvernostUdaju
n20:S
n5:entitaPredkladatele
n18:predkladatel
n5:idSjednocenehoVysledku
14114
n5:idVysledku
RIV/61989592:15110/14:33148910
n5:jazykVysledku
n15:eng
n5:klicovaSlova
Electrolytes; Protein stability; Electrochemical sensing; Ionic liquid; Protein
n5:klicoveSlovo
n9:Electrolytes n9:Protein n9:Protein%20stability n9:Electrochemical%20sensing n9:Ionic%20liquid
n5:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n5:kontrolniKodProRIV
[FAEC2C160ED3]
n5:nazevZdroje
Electrochimica Acta
n5:obor
n14:CG
n5:pocetDomacichTvurcuVysledku
3
n5:pocetTvurcuVysledku
4
n5:projekt
n17:EE2.3.20.0057
n5:rokUplatneniVysledku
n6:2014
n5:svazekPeriodika
126
n5:tvurceVysledku
Kubala, Martin Zatloukalová, Martina Vacek, Jan Vrba, Jiří
s:issn
0013-4686
s:numberOfPages
6
n13:doi
10.1016/j.electacta.2013.06.115
n19:organizacniJednotka
15110