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Statements

Subject Item
n2:RIV%2F61989592%3A15110%2F13%3A33146665%21RIV14-MZ0-15110___
rdf:type
n5:Vysledek skos:Concept
rdfs:seeAlso
http://biomed.papers.upol.cz/pdfs/bio/2013/04/18.pdf
dcterms:description
The aim of this study was to find possible relationship between ROS production measured by chemiluminescence and flow cytometry in human semen and sperm DNA damage. Study included 39 men from infertile couples and 23 fertile volunteers who served as a control group. Aliquot of neat semen was used for ROS detection by chemiluminescence. Aliquot of sperm suspension in phosphate buffered saline was used for the detection of ROS by flow cytometry. Another aliquot of sperm suspension was used for SCSA to measure DNA fragmentation index and High DNA stainability. DNA fragmentation index correlated negatively with sperm morphology and motility. High DNA stainability correlated positively with ROS production and negatively with sperm morphology and concentration. Relationship between DNA fragmentation index and ROS production did not reach statistical significance. The aim of this study was to find possible relationship between ROS production measured by chemiluminescence and flow cytometry in human semen and sperm DNA damage. Study included 39 men from infertile couples and 23 fertile volunteers who served as a control group. Aliquot of neat semen was used for ROS detection by chemiluminescence. Aliquot of sperm suspension in phosphate buffered saline was used for the detection of ROS by flow cytometry. Another aliquot of sperm suspension was used for SCSA to measure DNA fragmentation index and High DNA stainability. DNA fragmentation index correlated negatively with sperm morphology and motility. High DNA stainability correlated positively with ROS production and negatively with sperm morphology and concentration. Relationship between DNA fragmentation index and ROS production did not reach statistical significance.
dcterms:title
Relationship between reactive oxygen species production in human semen and sperm DNA damage assessed by Sperm Chromatin Structure Assay Relationship between reactive oxygen species production in human semen and sperm DNA damage assessed by Sperm Chromatin Structure Assay
skos:prefLabel
Relationship between reactive oxygen species production in human semen and sperm DNA damage assessed by Sperm Chromatin Structure Assay Relationship between reactive oxygen species production in human semen and sperm DNA damage assessed by Sperm Chromatin Structure Assay
skos:notation
RIV/61989592:15110/13:33146665!RIV14-MZ0-15110___
n5:predkladatel
n17:orjk%3A15110
n3:aktivita
n19:S n19:P
n3:aktivity
P(NT11083), S
n3:cisloPeriodika
4
n3:dodaniDat
n10:2014
n3:domaciTvurceVysledku
n4:1386581 n4:2796910 n4:1856057 n4:4891228
n3:druhVysledku
n18:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n14:predkladatel
n3:idSjednocenehoVysledku
102197
n3:idVysledku
RIV/61989592:15110/13:33146665
n3:jazykVysledku
n20:eng
n3:klicovaSlova
DNA damage; SCSA; ROS; human semen
n3:klicoveSlovo
n8:ROS n8:DNA%20damage n8:SCSA n8:human%20semen
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[840FF7D79D86]
n3:nazevZdroje
Biomedical Papers-Olomouc
n3:obor
n21:FK
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
8
n3:projekt
n13:NT11083
n3:rokUplatneniVysledku
n10:2013
n3:svazekPeriodika
157
n3:tvurceVysledku
Kopecká, Věra Řeřuchová, Marie Březinová, Jana Oborná, Ivana Aziz, Nabil Filipčíková, Radka Rybář, Roman Novotný, Jiří
n3:wos
000329091500019
s:issn
1213-8118
s:numberOfPages
4
n11:doi
10.5507/bp.2013.065
n15:organizacniJednotka
15110