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Statements

Subject Item
n2:RIV%2F61989592%3A15110%2F13%3A33145788%21RIV14-MZ0-15110___
rdf:type
skos:Concept n11:Vysledek
dcterms:description
Introduction. MicroRNAs (miRNAs) are small non-coding single-stranded RNA molecules that regulate gene expression at the post-transcriptional level. In the pathogenesis of chronic lymphocytic leukemia (CLL), miR-15a and miR-16-1 play an important role. These miRNAs are located on chromosome 13 in the 13q14.3 region, which is deleted in more than 55% of CLL patients. This aberration affects expression of miRNAs. Objectives. The study aimed at performing a molecular genetic analysis of miR-15a and miR-16-1 expression in a group of 39 patients diagnosed with CLL and determining the association between the expression of the two miRNAs and types of deletions in the 13q14 region. Methods. We used fluorescence in situ hybridiziation (FISH) for determination of mono- or biallelic deletion 13q and quantitative polymerase chain reaction (Q-RT-PCR) to revealed expression miR-15a and miR-16-1 in 39 patients suffering from CLL. Results. The analysis comprised 19 patients with monoallelic 13q14 deletion, 3 patients with biallelic deletion, 9 patients with both monoallelic and biallelic deletions, and 8 patients without 13q14 deletion serving as controls. The results showed different levels of miRNA expression in individual patients. Significantly higher normalized levels of miR-15a expression were found in the control group and patients with monoallelic 13q14 expression compared with patients with biallelic deletion. There was a significantly decreased expression of both miRNAs in patients with biallelic deletion of the 13q14 region but only when deletions were present in 77% or more of cells, as detected by fluorescent in situ hybridization (FISH). Introduction. MicroRNAs (miRNAs) are small non-coding single-stranded RNA molecules that regulate gene expression at the post-transcriptional level. In the pathogenesis of chronic lymphocytic leukemia (CLL), miR-15a and miR-16-1 play an important role. These miRNAs are located on chromosome 13 in the 13q14.3 region, which is deleted in more than 55% of CLL patients. This aberration affects expression of miRNAs. Objectives. The study aimed at performing a molecular genetic analysis of miR-15a and miR-16-1 expression in a group of 39 patients diagnosed with CLL and determining the association between the expression of the two miRNAs and types of deletions in the 13q14 region. Methods. We used fluorescence in situ hybridiziation (FISH) for determination of mono- or biallelic deletion 13q and quantitative polymerase chain reaction (Q-RT-PCR) to revealed expression miR-15a and miR-16-1 in 39 patients suffering from CLL. Results. The analysis comprised 19 patients with monoallelic 13q14 deletion, 3 patients with biallelic deletion, 9 patients with both monoallelic and biallelic deletions, and 8 patients without 13q14 deletion serving as controls. The results showed different levels of miRNA expression in individual patients. Significantly higher normalized levels of miR-15a expression were found in the control group and patients with monoallelic 13q14 expression compared with patients with biallelic deletion. There was a significantly decreased expression of both miRNAs in patients with biallelic deletion of the 13q14 region but only when deletions were present in 77% or more of cells, as detected by fluorescent in situ hybridization (FISH).
dcterms:title
Expression of miR-15a and miR-16-1 in patients with chronic lymphocytic leukemia Expression of miR-15a and miR-16-1 in patients with chronic lymphocytic leukemia
skos:prefLabel
Expression of miR-15a and miR-16-1 in patients with chronic lymphocytic leukemia Expression of miR-15a and miR-16-1 in patients with chronic lymphocytic leukemia
skos:notation
RIV/61989592:15110/13:33145788!RIV14-MZ0-15110___
n11:predkladatel
n12:orjk%3A15110
n3:aktivita
n17:S n17:P
n3:aktivity
P(NT13576), S
n3:cisloPeriodika
4
n3:dodaniDat
n18:2014
n3:domaciTvurceVysledku
n6:3461904 n6:9788662 n6:1030957 n6:8478325 n6:7405774
n3:druhVysledku
n16:J
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n5:predkladatel
n3:idSjednocenehoVysledku
74352
n3:idVysledku
RIV/61989592:15110/13:33145788
n3:jazykVysledku
n20:eng
n3:klicovaSlova
biallelic deletion; monoallelic deletion; 13q14 deletion; fluorescence in situ hybridization; miRNA expression; microRNA; chronic lymphocytic leukemia
n3:klicoveSlovo
n4:microRNA n4:biallelic%20deletion n4:miRNA%20expression n4:13q14%20deletion n4:chronic%20lymphocytic%20leukemia n4:monoallelic%20deletion n4:fluorescence%20in%20situ%20hybridization
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[8B2B2C468ED6]
n3:nazevZdroje
Biomedical Papers-Olomouc
n3:obor
n9:FD
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
10
n3:projekt
n7:NT13576
n3:rokUplatneniVysledku
n18:2013
n3:svazekPeriodika
157
n3:tvurceVysledku
Jarošová, Marie Pikalová, Zuzana Modrianský, Martin Holzerová, Milena Procházka, Vít Indrák, Karel Humplíková, L. Kollinerová, Soňa Papajík, Tomáš Divoká, Martina
s:issn
1213-8118
s:numberOfPages
10
n19:doi
10.5507/bp.2013.057
n15:organizacniJednotka
15110