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Statements

Subject Item
n2:RIV%2F61989592%3A15110%2F11%3A10223863%21RIV12-MSM-15110___
rdf:type
n12:Vysledek skos:Concept
dcterms:description
Visinin like protein 1 (VILIP-1) is a newly discovered CNS-abundant protein which has shown promise in experimental studies, for early stroke diagnosis. However, to date there is no clinical study that has measured VILIP-1 in sera as a marker of stroke. Aim. To develop an assay for the determination of VILIP-1 in human serum, and to investigate its clinical relevance as a marker of ischemic stroke. A new sandwich ELISA was developed, introduced and clinically tested. Mean spiking recovery was 98%. The mean recovery for dilution linearity was 93%. The limit of detection of the assay was 0.01 mcg/l; the intraassay and interassay coefficient of variation (CV) were always less than 10%. The study was approved by the Ethics Commission of the Hospital Šternberk, Czech Republic. A total of 17 healthy individuals (9 men and 8 women, age 64.0 +- 13.0) and 16 individuals with ischemic stroke (10 men and 6 women, age 63.0+-11.5) were recruited for our study. The criteria of stroke were proposed by the National Czech Standard. All individuals had blood samples drawn, and VILIP-1 analysis and CT and/or MRI were performed. VILIP-1 serum level significantly differentiated healthy subjects from patients with stroke (P{0.01). All individuals with stroke had VILIP-1 serum values higher than } 0.05 mcg/l, healthy had values below this value. The diagnostic efficacy of serum VILIP-1 was very significant (sensitivity 100%, specificity 100% at 0.093 mcg/l VILIP-1 serum values, AUC 1.0 (CI 0.93-1.0, P{0.01), Chi-squared in the frequency table was 33 (P{0.01). We have introduced a new analytical tool for the study of VILIP-1. Our results support the hypothesis that serum VILIP-1 may be associated with ischemic stroke. The ELISA VILIP-1 assay offers a new research tool for the diagnosis and pathophysiology of stroke and other CNS diseases. Visinin like protein 1 (VILIP-1) is a newly discovered CNS-abundant protein which has shown promise in experimental studies, for early stroke diagnosis. However, to date there is no clinical study that has measured VILIP-1 in sera as a marker of stroke. Aim. To develop an assay for the determination of VILIP-1 in human serum, and to investigate its clinical relevance as a marker of ischemic stroke. A new sandwich ELISA was developed, introduced and clinically tested. Mean spiking recovery was 98%. The mean recovery for dilution linearity was 93%. The limit of detection of the assay was 0.01 mcg/l; the intraassay and interassay coefficient of variation (CV) were always less than 10%. The study was approved by the Ethics Commission of the Hospital Šternberk, Czech Republic. A total of 17 healthy individuals (9 men and 8 women, age 64.0 +- 13.0) and 16 individuals with ischemic stroke (10 men and 6 women, age 63.0+-11.5) were recruited for our study. The criteria of stroke were proposed by the National Czech Standard. All individuals had blood samples drawn, and VILIP-1 analysis and CT and/or MRI were performed. VILIP-1 serum level significantly differentiated healthy subjects from patients with stroke (P{0.01). All individuals with stroke had VILIP-1 serum values higher than } 0.05 mcg/l, healthy had values below this value. The diagnostic efficacy of serum VILIP-1 was very significant (sensitivity 100%, specificity 100% at 0.093 mcg/l VILIP-1 serum values, AUC 1.0 (CI 0.93-1.0, P{0.01), Chi-squared in the frequency table was 33 (P{0.01). We have introduced a new analytical tool for the study of VILIP-1. Our results support the hypothesis that serum VILIP-1 may be associated with ischemic stroke. The ELISA VILIP-1 assay offers a new research tool for the diagnosis and pathophysiology of stroke and other CNS diseases.
dcterms:title
DETERMINATION OF SERUM VISININ LIKE PROTEIN-1 AND ITS POTENTIAL FOR THE DIAGNOSIS OF BRAIN INJURY DUE TO THE STROKE - A PILOT STUDY DETERMINATION OF SERUM VISININ LIKE PROTEIN-1 AND ITS POTENTIAL FOR THE DIAGNOSIS OF BRAIN INJURY DUE TO THE STROKE - A PILOT STUDY
skos:prefLabel
DETERMINATION OF SERUM VISININ LIKE PROTEIN-1 AND ITS POTENTIAL FOR THE DIAGNOSIS OF BRAIN INJURY DUE TO THE STROKE - A PILOT STUDY DETERMINATION OF SERUM VISININ LIKE PROTEIN-1 AND ITS POTENTIAL FOR THE DIAGNOSIS OF BRAIN INJURY DUE TO THE STROKE - A PILOT STUDY
skos:notation
RIV/61989592:15110/11:10223863!RIV12-MSM-15110___
n12:predkladatel
n19:orjk%3A15110
n3:aktivita
n18:Z n18:I
n3:aktivity
I, Z(MSM6198959216)
n3:cisloPeriodika
3
n3:dodaniDat
n16:2012
n3:domaciTvurceVysledku
n14:8313334 n14:5477204 n14:9101934
n3:druhVysledku
n9:J
n3:duvernostUdaju
n15:S
n3:entitaPredkladatele
n10:predkladatel
n3:idSjednocenehoVysledku
193885
n3:idVysledku
RIV/61989592:15110/11:10223863
n3:jazykVysledku
n7:eng
n3:klicovaSlova
ELISA; Stroke; Visinin like protein 1; VILIP-1
n3:klicoveSlovo
n5:ELISA n5:VILIP-1 n5:Stroke n5:Visinin%20like%20protein%201
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[E00C877CE815]
n3:nazevZdroje
Biomedical Papers-Olomouc
n3:obor
n17:CE
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
4
n3:rokUplatneniVysledku
n16:2011
n3:svazekPeriodika
155
n3:tvurceVysledku
Karpíšek, Michal Stejskal, David Švesták, Marek Sporová, Lenka
n3:zamer
n4:MSM6198959216
s:issn
1213-8118
s:numberOfPages
5
n13:organizacniJednotka
15110