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Statements

Subject Item
n2:RIV%2F61989592%3A15110%2F09%3A00009785%21RIV10-MZ0-15110___
rdf:type
n12:Vysledek skos:Concept
dcterms:description
Background: It is generally accepted that oxidative stress is an important factor in male infertility because it may impair the physiological function of spermatozoa at the molecular level. Nevertheless, although several approaches have been reported, the imbalance between production of reactive oxygen species (ROS) and activity of the antioxidant defense system in semen is difficult to investigate and remains poorly understood. Methods: This study compares measurement of ROS production in neat semen and in washed spermatozoa obtained from the same ejaculate, and suspended in phosphate buffered saline using exactly the same luminol-mediated chemiluminescence method. Ninety one samples were obtained from males of infertile couples and 34 from volunteers with proven fertility. Results: As expected, ROS levels were markedly lower in neat semen than in washed spermatozoa suspensions where seminal plasma with its potent antioxidant capacity was removed. In the cases of both neat semen and washed sperma Background: It is generally accepted that oxidative stress is an important factor in male infertility because it may impair the physiological function of spermatozoa at the molecular level. Nevertheless, although several approaches have been reported, the imbalance between production of reactive oxygen species (ROS) and activity of the antioxidant defense system in semen is difficult to investigate and remains poorly understood. Methods: This study compares measurement of ROS production in neat semen and in washed spermatozoa obtained from the same ejaculate, and suspended in phosphate buffered saline using exactly the same luminol-mediated chemiluminescence method. Ninety one samples were obtained from males of infertile couples and 34 from volunteers with proven fertility. Results: As expected, ROS levels were markedly lower in neat semen than in washed spermatozoa suspensions where seminal plasma with its potent antioxidant capacity was removed. In the cases of both neat semen and washed sperma
dcterms:title
The Measurement of Reactive Oxygen Species in Human Neat Semen and in Suspended Spermatozoa: A Comparison The Measurement of Reactive Oxygen Species in Human Neat Semen and in Suspended Spermatozoa: A Comparison
skos:prefLabel
The Measurement of Reactive Oxygen Species in Human Neat Semen and in Suspended Spermatozoa: A Comparison The Measurement of Reactive Oxygen Species in Human Neat Semen and in Suspended Spermatozoa: A Comparison
skos:notation
RIV/61989592:15110/09:00009785!RIV10-MZ0-15110___
n3:aktivita
n8:P
n3:aktivity
P(1A8621)
n3:cisloPeriodika
10
n3:dodaniDat
n6:2010
n3:domaciTvurceVysledku
n13:4607120 n13:5281415 n13:4891228 n13:4452240 n13:2796910 n13:7212186
n3:druhVysledku
n4:J
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n10:predkladatel
n3:idSjednocenehoVysledku
325048
n3:idVysledku
RIV/61989592:15110/09:00009785
n3:jazykVysledku
n18:eng
n3:klicovaSlova
oxidative stress; reactive oxygen species; luminol chemiluminescence assay; male infertility; human semen
n3:klicoveSlovo
n9:oxidative%20stress n9:reactive%20oxygen%20species n9:male%20infertility n9:luminol%20chemiluminescence%20assay n9:human%20semen
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[7976553F5856]
n3:nazevZdroje
Reproductive biology and endocrinology
n3:obor
n16:FK
n3:pocetDomacichTvurcuVysledku
6
n3:pocetTvurcuVysledku
6
n3:projekt
n15:1A8621
n3:rokUplatneniVysledku
n6:2009
n3:svazekPeriodika
7
n3:tvurceVysledku
Oborná, Ivana Svobodová, Magda Radová, Lenka Novotný, Jiří Březinová, Jana Fingerová, Helena
s:issn
1477-7827
s:numberOfPages
6
n17:organizacniJednotka
15110