This HTML5 document contains 44 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n11http://localhost/temp/predkladatel/
n17http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n13http://linked.opendata.cz/ontology/domain/vavai/
n9http://linked.opendata.cz/resource/domain/vavai/zamer/
n18http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F61989592%3A15110%2F08%3A00010974%21RIV10-MSM-15110___/
shttp://schema.org/
n4http://linked.opendata.cz/ontology/domain/vavai/riv/
skoshttp://www.w3.org/2004/02/skos/core#
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n8http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n14http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n12http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n5http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n15http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n7http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n6http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F61989592%3A15110%2F08%3A00010974%21RIV10-MSM-15110___
rdf:type
skos:Concept n13:Vysledek
dcterms:description
ATR is a protein kinase that orchestrates the cellular response to replication problems and DNA damage. HCLK2 has previously been reported to stabilize ATR and Chk1. Here we provide evidence that human HCLK2 acts at an early step in the ATR signaling pathway and contributes to full-scale activation of ATR kinase activity.Weshow that HCLK2 forms a complex with ATR-ATRIP and the ATR activator TopBP1. We demonstrate that HCLK2-induced ATR kinase activity toward substrates requires TopBP1 and vice versa and provides evidence that HCLK2 facilitates efficient ATR-TopBP1 association. Consistent with its role in ATR activation, HCLK2 depletion severely impaired phosphorylation of multiple ATR targets including Chk1, Nbs1, and Smc1 after DNA damage. We show that HCLK2 is required for and stimulates ATR autophosphorylation and activity toward different substrates in vitro. Furthermore, HCLK2 depletion abrogated the G2 checkpoint and decreased survival of cells after exposure to DNA damaging agents and replicati ATR is a protein kinase that orchestrates the cellular response to replication problems and DNA damage. HCLK2 has previously been reported to stabilize ATR and Chk1. Here we provide evidence that human HCLK2 acts at an early step in the ATR signaling pathway and contributes to full-scale activation of ATR kinase activity.Weshow that HCLK2 forms a complex with ATR-ATRIP and the ATR activator TopBP1. We demonstrate that HCLK2-induced ATR kinase activity toward substrates requires TopBP1 and vice versa and provides evidence that HCLK2 facilitates efficient ATR-TopBP1 association. Consistent with its role in ATR activation, HCLK2 depletion severely impaired phosphorylation of multiple ATR targets including Chk1, Nbs1, and Smc1 after DNA damage. We show that HCLK2 is required for and stimulates ATR autophosphorylation and activity toward different substrates in vitro. Furthermore, HCLK2 depletion abrogated the G2 checkpoint and decreased survival of cells after exposure to DNA damaging agents and replicati
dcterms:title
HCLK2 Is Required for Activity of the DNA Damage Response Kinase ATR HCLK2 Is Required for Activity of the DNA Damage Response Kinase ATR
skos:prefLabel
HCLK2 Is Required for Activity of the DNA Damage Response Kinase ATR HCLK2 Is Required for Activity of the DNA Damage Response Kinase ATR
skos:notation
RIV/61989592:15110/08:00010974!RIV10-MSM-15110___
n4:aktivita
n12:Z
n4:aktivity
Z(MSM6198959216)
n4:cisloPeriodika
284
n4:dodaniDat
n6:2010
n4:domaciTvurceVysledku
n17:6201822
n4:druhVysledku
n15:J
n4:duvernostUdaju
n14:S
n4:entitaPredkladatele
n18:predkladatel
n4:idSjednocenehoVysledku
369858
n4:idVysledku
RIV/61989592:15110/08:00010974
n4:jazykVysledku
n5:eng
n4:klicovaSlova
ATR; DNA damage; replicative stress; checkpoint signaling
n4:klicoveSlovo
n8:DNA%20damage n8:ATR n8:replicative%20stress n8:checkpoint%20signaling
n4:kodStatuVydavatele
US - Spojené státy americké
n4:kontrolniKodProRIV
[267FF4CB8E1B]
n4:nazevZdroje
Journal of Biological Chemistry
n4:obor
n7:EB
n4:pocetDomacichTvurcuVysledku
1
n4:pocetTvurcuVysledku
7
n4:rokUplatneniVysledku
n6:2008
n4:svazekPeriodika
13
n4:tvurceVysledku
Bødtker Schou, Kenneth Lukas, Jiří Falck, Jacob Freire, Raimundo Larsen, Dorthe Helena Rendtlew Danielsen, Jannie M. Bártek, Jiří
n4:zamer
n9:MSM6198959216
s:issn
0021-9258
s:numberOfPages
8
n11:organizacniJednotka
15110