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Statements

Subject Item
n2:RIV%2F61989592%3A15110%2F08%3A00006999%21RIV09-MSM-15110___
rdf:type
skos:Concept n10:Vysledek
dcterms:description
Kvarterní benzo[c]fenanthridinový alkaloid chelerythrin vykazuje řadu biologických aktivit včetně cytotoxicity vůči normálním a nádorovým buňkám. Méně je však známo o biologické aktivitě dihydrochelerythrinu, produktu redukce chelerythrinu. My jsme se zabývali srovnáním cytotoxicity chelerythrinu a dihydrochelerythrinu v leukemických buňkách HL-60. Při inkubaci po dobu 4 h chelerythrin snižoval životnost buněk, přičemž hodnota IC50 získaná MTT metodou byla 2,6 ?mol/l. Dihydrochelerythrin byl méně cytotoxický, když při koncentraci 20 ?mol/l snížil po 24 h životnost pouze na 53%. Pokles životnosti vlivem obou alkaloidů byl doprovázen apoptotickými změnami zahrnujícími ztrátu mitochondriálního membránového poteciálu, aktivaci kaspasy-9 a -3 a degradaci DNA. Pouze chelerythrin navíc zvýšil také aktivitu kaspasy-8. Indukce apoptosy a nekrosy vlivem chelerythrinu a dihydrochelerythrinu byla potvrzena průtokovou cytometrií využívající značení buněk annexinem V a propidium jodidem. Oba alkaloidy také způsobov A quaternary benzo[c]phenanthridine alkaloid chelerythrine displays a wide range of biological activities including cytotoxicity to normal and cancer cells. In contrast, less is known about the biological activity of dihydrochelerythrine, a product of chelerythrine reduction. We examined the cytotoxicity of chelerythrine and dihydrochelerythrine in human promyelocytic leukemia HL-60 cells. After 4h of treatment, chelerythrine induced a dose-dependent decrease in the cell viability with IC50 of 2.6 microM as shown by MTT reduction assay. Dihydrochelerythrine appeared to be less cytotoxic since the viability of cells exposed to 20 microM dihydrochelerythrine for 24h was reduced only to 53%. Decrease in the viability induced by both alkaloids was accompanied by apoptotic events including the dissipation of mitochondrial membrane potential, activation of caspase-9 and -3, and appearance of cells with sub-G1 DNA. Moreover, chelerythrine, but not dihydrochelerythrine, elevated the activity of caspase-8. A d A quaternary benzo[c]phenanthridine alkaloid chelerythrine displays a wide range of biological activities including cytotoxicity to normal and cancer cells. In contrast, less is known about the biological activity of dihydrochelerythrine, a product of chelerythrine reduction. We examined the cytotoxicity of chelerythrine and dihydrochelerythrine in human promyelocytic leukemia HL-60 cells. After 4h of treatment, chelerythrine induced a dose-dependent decrease in the cell viability with IC50 of 2.6 microM as shown by MTT reduction assay. Dihydrochelerythrine appeared to be less cytotoxic since the viability of cells exposed to 20 microM dihydrochelerythrine for 24h was reduced only to 53%. Decrease in the viability induced by both alkaloids was accompanied by apoptotic events including the dissipation of mitochondrial membrane potential, activation of caspase-9 and -3, and appearance of cells with sub-G1 DNA. Moreover, chelerythrine, but not dihydrochelerythrine, elevated the activity of caspase-8. A d
dcterms:title
Chelerythrine and dihydrochelerythrine induce G1 phase arrest and bimodal cell death in human leukemia HL-60 cells Chelerythrine and dihydrochelerythrine induce G1 phase arrest and bimodal cell death in human leukemia HL-60 cells Chelerythrin a dihydrochelerythrin způsobují v buňkách HL-60 zastavení buněčného cyklu v G1 fázi a bimodální buněčnou smrt
skos:prefLabel
Chelerythrine and dihydrochelerythrine induce G1 phase arrest and bimodal cell death in human leukemia HL-60 cells Chelerythrine and dihydrochelerythrine induce G1 phase arrest and bimodal cell death in human leukemia HL-60 cells Chelerythrin a dihydrochelerythrin způsobují v buňkách HL-60 zastavení buněčného cyklu v G1 fázi a bimodální buněčnou smrt
skos:notation
RIV/61989592:15110/08:00006999!RIV09-MSM-15110___
n5:aktivita
n12:P n12:Z
n5:aktivity
P(GP303/06/P193), Z(MSM6198959216)
n5:cisloPeriodika
4
n5:dodaniDat
n13:2009
n5:domaciTvurceVysledku
n9:2514559 n9:5773458 n9:1030957
n5:druhVysledku
n14:J
n5:duvernostUdaju
n15:S
n5:entitaPredkladatele
n8:predkladatel
n5:idSjednocenehoVysledku
359698
n5:idVysledku
RIV/61989592:15110/08:00006999
n5:jazykVysledku
n6:eng
n5:klicovaSlova
Apoptosis; Cell cycle; Chelerythrine; Cytotoxicity; Dihydrochelerythrine; HL-60 cells; Mitochondrial membrane potential
n5:klicoveSlovo
n11:Mitochondrial%20membrane%20potential n11:HL-60%20cells n11:Dihydrochelerythrine n11:Apoptosis n11:Cytotoxicity n11:Chelerythrine n11:Cell%20cycle
n5:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n5:kontrolniKodProRIV
[32D1930B1FC6]
n5:nazevZdroje
Toxicology in Vitro
n5:obor
n16:CE
n5:pocetDomacichTvurcuVysledku
3
n5:pocetTvurcuVysledku
5
n5:projekt
n7:GP303%2F06%2FP193
n5:rokUplatneniVysledku
n13:2008
n5:svazekPeriodika
22
n5:tvurceVysledku
Ulrichová, Jitka Doležel, Petr Modrianský, Martin Vrba, Jiří Vičar, Jaroslav
n5:zamer
n18:MSM6198959216
s:issn
0887-2333
s:numberOfPages
10
n19:organizacniJednotka
15110