This HTML5 document contains 58 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n16http://localhost/temp/predkladatel/
n17http://linked.opendata.cz/resource/domain/vavai/projekt/
n4http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n15http://linked.opendata.cz/ontology/domain/vavai/
n11http://linked.opendata.cz/resource/domain/vavai/zamer/
shttp://schema.org/
skoshttp://www.w3.org/2004/02/skos/core#
n3http://linked.opendata.cz/ontology/domain/vavai/riv/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n7http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n13http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n18http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n9http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n19http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n5http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n10http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F61989592%3A15110%2F07%3A00004435%21RIV08-MSM-15110___/
n8http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F61989592%3A15110%2F07%3A00004435%21RIV08-MSM-15110___
rdf:type
skos:Concept n15:Vysledek
dcterms:description
To investigate the effect of dehydroepiandrosterone (DHEA) on intracellular mRNA levels of peroxisome proliferator-activated receptors (PPARs) and PPAR-gamma coactivators (PGCs), we conducted a quantitative real-time RT-PCR study using HepG2 cells. Treatment with 100 micromol/l DHEA for 2-20 h caused a time-dependent elevation of mRNA levels in the cells. Upon 20 h, PPAR-alpha, -gamma1, and -gamma2 mRNAs and PGC-1alpha and -1beta mRNAs increased to 157, 161, 155, 656, and 475% of control levels, respectively (p < 0.05 each). Treatment with actinomycin D for 2.5-8 h revealed a significant stabilization effect of DHEA on PPAR-gamma1 and PGC-1alpha mRNAs at both 2.5 and 8 h incubation periods and a mild but significant stabilization effect on PGC-1beta mRNA at the 8 h incubation period suggesting that DHEA can modulate turnover of these mRNA transcripts. Basal mRNA levels of PPAR-alpha and PGC-1alpha were significantly suppressed upon 20 h treatment with cycloheximide, while those of PPAR-gamma1, -gam To investigate the effect of dehydroepiandrosterone (DHEA) on intracellular mRNA levels of peroxisome proliferator-activated receptors (PPARs) and PPAR-gamma coactivators (PGCs), we conducted a quantitative real-time RT-PCR study using HepG2 cells. Treatment with 100 micromol/l DHEA for 2-20 h caused a time-dependent elevation of mRNA levels in the cells. Upon 20 h, PPAR-alpha, -gamma1, and -gamma2 mRNAs and PGC-1alpha and -1beta mRNAs increased to 157, 161, 155, 656, and 475% of control levels, respectively (p < 0.05 each). Treatment with actinomycin D for 2.5-8 h revealed a significant stabilization effect of DHEA on PPAR-gamma1 and PGC-1alpha mRNAs at both 2.5 and 8 h incubation periods and a mild but significant stabilization effect on PGC-1beta mRNA at the 8 h incubation period suggesting that DHEA can modulate turnover of these mRNA transcripts. Basal mRNA levels of PPAR-alpha and PGC-1alpha were significantly suppressed upon 20 h treatment with cycloheximide, while those of PPAR-gamma1, -gam V této práci jsme stanovili účinky dehydroepiandrosteronu (DHEA) na intracelulární hladiny mRNA receptorů aktivovanými peroxisomálními proliferátory (PPAR) a PPAR-γ koaktivátorů v buněčné linii HepG2 za pomoci metody kvantitativní real-time RT-PCR. Ovlivnění 100 μmol/l DHEA po dobu 2-20 hodin vedlo ke zvýšení hladin sledovaných mRNA s časem. Po 20 hodinách jsme pozorovali zvýšení mRNA PPAR-α, -γ1 a ?γ2 i koaktivátorů PGC-1α a -1β na 157, 161, 155, 656 a 475 % (p < 0,05) oproti kontrole. Ovlivnění aktinomycinem D po dobu 2,5 ? 8 hodin odhalilo signifikantní stabilizační účinek DHEA na mRNA PPAR-γ1 a PGC-1α v obou časových intervalech. 8-hodinová inkubace s aktinomycinem D také vyvolala mírný, ale signifikantní stabilizační účinek na PGC-1β. Z výsledků vyplývá, že DHEA může řídit přeměnu těchto transkriptů. Po 20-hodinovém působeni cykloheximidu došlo k signifikantnímu poklesu bazálních hladin mRNA PPAR-α a PGC-1α, zatímco hladiny mRNA PPAR
dcterms:title
Účinky dehydroepiandrosteronu na úroveň mRNA PPAR a jejich kokoaktivátorů v lidských hepatomových buňkách HepG2 Dehydroepiandrosterone effects on the mRNA levels of peroxisome proliferator-activated receptors and their coactivators in human hepatoma HepG2 cells Dehydroepiandrosterone effects on the mRNA levels of peroxisome proliferator-activated receptors and their coactivators in human hepatoma HepG2 cells
skos:prefLabel
Dehydroepiandrosterone effects on the mRNA levels of peroxisome proliferator-activated receptors and their coactivators in human hepatoma HepG2 cells Dehydroepiandrosterone effects on the mRNA levels of peroxisome proliferator-activated receptors and their coactivators in human hepatoma HepG2 cells Účinky dehydroepiandrosteronu na úroveň mRNA PPAR a jejich kokoaktivátorů v lidských hepatomových buňkách HepG2
skos:notation
RIV/61989592:15110/07:00004435!RIV08-MSM-15110___
n3:strany
268-274
n3:aktivita
n9:S n9:P n9:Z
n3:aktivity
P(OC 153), S, Z(MSM6198959205)
n3:cisloPeriodika
4
n3:dodaniDat
n8:2008
n3:domaciTvurceVysledku
n4:5879426 n4:2718898 n4:6974899 n4:6087914 n4:5047633 n4:1579843 n4:5628288
n3:druhVysledku
n5:J
n3:duvernostUdaju
n13:S
n3:entitaPredkladatele
n10:predkladatel
n3:idSjednocenehoVysledku
416056
n3:idVysledku
RIV/61989592:15110/07:00004435
n3:jazykVysledku
n18:eng
n3:klicovaSlova
Dehydroepiandrosterone; Peroxisome proliferator-activated receptor; PPAR-gamma coactivator; mRNA stability; HepG2 cells
n3:klicoveSlovo
n7:mRNA%20stability n7:Dehydroepiandrosterone n7:HepG2%20cells n7:Peroxisome%20proliferator-activated%20receptor n7:PPAR-gamma%20coactivator
n3:kodStatuVydavatele
SK - Slovenská republika
n3:kontrolniKodProRIV
[D1C3DB9845C1]
n3:nazevZdroje
General Physiology and Biophysics
n3:obor
n19:EB
n3:pocetDomacichTvurcuVysledku
7
n3:pocetTvurcuVysledku
7
n3:projekt
n17:OC%20153
n3:rokUplatneniVysledku
n8:2007
n3:svazekPeriodika
26
n3:tvurceVysledku
Uherková, Lenka Veselý, Jaroslav Rypka, Miroslav Bogdanová, Kateřina Riegrová, Dagmar Červenková, Kateřina Poczatková, Hana
n3:zamer
n11:MSM6198959205
s:issn
0231-5882
s:numberOfPages
7
n16:organizacniJednotka
15110