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Statements

Subject Item
n2:RIV%2F61989592%3A15110%2F06%3A00003481%21RIV07-MSM-15110___
rdf:type
n10:Vysledek skos:Concept
dcterms:description
We compared the effects of chelerythrine (CHE) and sanguinarine (SA) on human prostate cancer cell lines (LNCaP and DU-145) and primary culture of human gingival fibroblasts. CHE and SA treatment of cell lines for 24 h resulted in (1) inhibition of cell viability in a dose-dependent manner in all tested cells (as evaluated by MTT test and bromodeoxyuridine incorporation assay); (2) dose-dependent increase in DNA damage in all tested cells (as evaluated by DNA comet assay); (3) changes in apoptosis (assessed by western blot analysis and TUNEL assay); and (4) significant induction of cyclin kinase inhibitors p21(Waf1/Cip1) and p27(Kip1) in prostate cancer cells (identified by western blot analysis). Our study demonstrates that CHE had significant cytotoxic effect, independent of p53 and androgen status, on human prostate cancer cell lines. Normal gingival fibroblasts and DU-145 cells were more sensitive to the treatment with both alkaloids than were LNCaP cells. CHE and SA may be prospective natural mol V této studii jsme porovnávali vliv chelerythrinu (CHE) a sanguinarinu (SA) na lidské prostatické nádorové buněčné linie (LNCaP a DU-145) a primární kulturu lidských gingiválních fibroblastů. Buňky byly vystaveny 24 h působení SA a CHE. Poté bylo pozorováno (1) snížení životnosti buněk v závislosti na koncentraci SA a CHE u všech testovaných buněčných modelů (hodnoceno MTT testem a inkorporací bromdeoxyuridinu do buněk); (2) nárůst poškození DNA v závislosti na koncentraci látek u všech testovaných buněk (hodnoceno metodou Comet essay); (3) změny v hladinách proteinů zapojených do procesu apoptózy (sledované western blot analýzou a metodou TUNEL); (4) signifikantní indukce inhibitorů cyklin-dependentních kinas p21Waf1/Cip1 a p27Kip1 u prostatických nádorových buněčných linií (stanovené western blot analýzou). V této studii jsme dokázaly, že CHE má statisticky významný cytotoxický účinek na prostatické nádorové buněčné linie, nezávislý na obsahu proteinu p53 a androgenu. Normální gingivální fibroblast We compared the effects of chelerythrine (CHE) and sanguinarine (SA) on human prostate cancer cell lines (LNCaP and DU-145) and primary culture of human gingival fibroblasts. CHE and SA treatment of cell lines for 24 h resulted in (1) inhibition of cell viability in a dose-dependent manner in all tested cells (as evaluated by MTT test and bromodeoxyuridine incorporation assay); (2) dose-dependent increase in DNA damage in all tested cells (as evaluated by DNA comet assay); (3) changes in apoptosis (assessed by western blot analysis and TUNEL assay); and (4) significant induction of cyclin kinase inhibitors p21(Waf1/Cip1) and p27(Kip1) in prostate cancer cells (identified by western blot analysis). Our study demonstrates that CHE had significant cytotoxic effect, independent of p53 and androgen status, on human prostate cancer cell lines. Normal gingival fibroblasts and DU-145 cells were more sensitive to the treatment with both alkaloids than were LNCaP cells. CHE and SA may be prospective natural mol
dcterms:title
The effect of chelerythrine on cell growth, apoptosis, and cell cycle in human normal and cancer cells in comparison with sanguinarine The effect of chelerythrine on cell growth, apoptosis, and cell cycle in human normal and cancer cells in comparison with sanguinarine Porovnání vlivu chelerythrinu a sanguinarine na buněčný růst, apoptózu a buněčný cyklus u lidských gingiválních fibroblastů a nádorových buněk
skos:prefLabel
Porovnání vlivu chelerythrinu a sanguinarine na buněčný růst, apoptózu a buněčný cyklus u lidských gingiválních fibroblastů a nádorových buněk The effect of chelerythrine on cell growth, apoptosis, and cell cycle in human normal and cancer cells in comparison with sanguinarine The effect of chelerythrine on cell growth, apoptosis, and cell cycle in human normal and cancer cells in comparison with sanguinarine
skos:notation
RIV/61989592:15110/06:00003481!RIV07-MSM-15110___
n4:strany
439-453
n4:aktivita
n18:Z
n4:aktivity
Z(MSM6198959216)
n4:cisloPeriodika
6
n4:dodaniDat
n8:2007
n4:domaciTvurceVysledku
n9:2706571 n9:2495058 n9:2514559 n9:2619725
n4:druhVysledku
n11:J
n4:duvernostUdaju
n7:S
n4:entitaPredkladatele
n12:predkladatel
n4:idSjednocenehoVysledku
472985
n4:idVysledku
RIV/61989592:15110/06:00003481
n4:jazykVysledku
n14:eng
n4:klicovaSlova
Apoptosis; Cell cycle; Chelerythrine; Cytotoxicity; DNA damage; Sanguinarine
n4:klicoveSlovo
n5:Apoptosis n5:Cytotoxicity n5:Cell%20cycle n5:Sanguinarine n5:Chelerythrine n5:DNA%20damage
n4:kodStatuVydavatele
NL - Nizozemsko
n4:kontrolniKodProRIV
[1D87BF8EDFEB]
n4:nazevZdroje
Cell Biology and Toxicology
n4:obor
n15:CE
n4:pocetDomacichTvurcuVysledku
4
n4:pocetTvurcuVysledku
4
n4:rokUplatneniVysledku
n8:2006
n4:svazekPeriodika
22
n4:tvurceVysledku
Ulrichová, Jitka Malíková, Jana Zdařilová, Adéla Hlobilková, Alice
n4:zamer
n16:MSM6198959216
s:issn
0742-2091
s:numberOfPages
15
n17:organizacniJednotka
15110