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Statements

Subject Item
n2:RIV%2F61989592%3A15110%2F06%3A00003478%21RIV07-MSM-15110___
rdf:type
skos:Concept n5:Vysledek
dcterms:description
Putative interactions between quaternary benzo[c]phenanthridine alkaloid sanguinarine (SA) and aryl hydrocarbon receptor/cytochrome P450 CYP1A (AhR/CYP1A) regulatory pathway are the subject of perpetual disputations. The role of CYP1A enzymes and AhR receptor in SA cytotoxicity was anticipated. In this paper, we tested, whether selected inducers of CYP enzymes modulate cytotoxicity of SA in primary cultures of rat hepatocytes. Cells were challenged 48h with dioxin (TCDD; 5nM), phenobarbital (PB; 500microM) or DMSO prior to the treatment with SA. SA itself displayed time- and dose-dependent cytotoxicity as revealed by lactate dehydrogenase leakage into the medium and MTT test. Pre-treatment of hepatocytes with TCDD and/or PB significantly attenuated SA cytotoxicity, the effects being more pronounced at lower concentrations of SA and shorter periods of incubation. We assumed involvement of CYP1A enzymes in diminution of SA cytotoxicity. Surprisingly, co-treatment with SA and furafylline, an inhibitor of Putative interactions between quaternary benzo[c]phenanthridine alkaloid sanguinarine (SA) and aryl hydrocarbon receptor/cytochrome P450 CYP1A (AhR/CYP1A) regulatory pathway are the subject of perpetual disputations. The role of CYP1A enzymes and AhR receptor in SA cytotoxicity was anticipated. In this paper, we tested, whether selected inducers of CYP enzymes modulate cytotoxicity of SA in primary cultures of rat hepatocytes. Cells were challenged 48h with dioxin (TCDD; 5nM), phenobarbital (PB; 500microM) or DMSO prior to the treatment with SA. SA itself displayed time- and dose-dependent cytotoxicity as revealed by lactate dehydrogenase leakage into the medium and MTT test. Pre-treatment of hepatocytes with TCDD and/or PB significantly attenuated SA cytotoxicity, the effects being more pronounced at lower concentrations of SA and shorter periods of incubation. We assumed involvement of CYP1A enzymes in diminution of SA cytotoxicity. Surprisingly, co-treatment with SA and furafylline, an inhibitor of Možné interakce mezi kvartérním bezofenanthridinovým alkaloidem sanguinarinem (SA) a regulační drahou aryluhlovodíkový receptor/cytochrom P450 CYP1A (AhR/CYP1A) jsou předmětem neutuchajících diskuzí. Předpokládá se úloha CYP1A enzymů a AhR receptoru v toxicitě SA. V této práci jsme testovali, zda vybrané induktory CYP enzymů modulují cytotoxicitu SA v primárních kulturách potkaních hepatocytů. Buňky byly pre-inkubovány 48 hod s dioxinem (TCDD; 5 nM), fenobarbitalem (PB; 500 mM) nebo DMSO před vlastní inkubací s SA. Samotný SA vykazoval časově a koncentračně závislou cytotoxicitu, monitorováno jako aktivita laktát dehydrogenasy v médiu a MTT test. Preinkubace hepatocytů s TCDD nebo PB signifikantně snížila cytotoxicitu SA; účinky byly výraznější v nižších koncentracích SA a v kratších periodách inkubace. Předpokládali jsme, že zymy CYP1A jsou zapojeny do vymizení cytotoxicity SA. Bylo překvapivé, že inkubace buněk se SA společně s furafylinem, inhibitorem CYP1A enzymů, ještě více snížila cytotoxicitu S
dcterms:title
Cytotoxicity of sanguinarine in primary rat hepatocytes is attenuated by dioxin and phenobarbital Cytotoxicity of sanguinarine in primary rat hepatocytes is attenuated by dioxin and phenobarbital Cytotoxicita sanguinarinu v primárních potkaních hepatocytech je snížena dioxinem a fenobarbitalem
skos:prefLabel
Cytotoxicita sanguinarinu v primárních potkaních hepatocytech je snížena dioxinem a fenobarbitalem Cytotoxicity of sanguinarine in primary rat hepatocytes is attenuated by dioxin and phenobarbital Cytotoxicity of sanguinarine in primary rat hepatocytes is attenuated by dioxin and phenobarbital
skos:notation
RIV/61989592:15110/06:00003478!RIV07-MSM-15110___
n4:strany
282-288
n4:aktivita
n18:Z
n4:aktivity
Z(MSM6198959216)
n4:cisloPeriodika
3
n4:dodaniDat
n8:2007
n4:domaciTvurceVysledku
n6:8738394 n6:8321124 n6:8069239 n6:2706571 n6:2514559
n4:druhVysledku
n13:J
n4:duvernostUdaju
n14:S
n4:entitaPredkladatele
n7:predkladatel
n4:idSjednocenehoVysledku
470277
n4:idVysledku
RIV/61989592:15110/06:00003478
n4:jazykVysledku
n9:eng
n4:klicovaSlova
Sanguinarine; Cytochrome P450; Induction; Cytotoxicity
n4:klicoveSlovo
n11:Sanguinarine n11:Cytochrome%20P450 n11:Cytotoxicity n11:Induction
n4:kodStatuVydavatele
IE - Irsko
n4:kontrolniKodProRIV
[76AF4FDA3445]
n4:nazevZdroje
Toxicology Letters
n4:obor
n15:CE
n4:pocetDomacichTvurcuVysledku
5
n4:pocetTvurcuVysledku
6
n4:rokUplatneniVysledku
n8:2006
n4:svazekPeriodika
165
n4:tvurceVysledku
Anzenbacherová, Eva Šimánek, Vilím Zdařilová, Adéla Šperlíková, Lucie Ulrichová, Jitka Dvořák, Zdeněk
n4:zamer
n17:MSM6198959216
s:issn
0378-4274
s:numberOfPages
7
n16:organizacniJednotka
15110