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Statements

Subject Item
n2:RIV%2F61989592%3A15110%2F06%3A00002714%21RIV07-MSM-15110___
rdf:type
skos:Concept n16:Vysledek
dcterms:description
Cryopreservation oVers the potential to maximize the use and availability of biological materials that have a limited supply. This study demonstrates an enhanced technique for the parallel cryopreservation of a series of liver tissue slices using a tray modeled from aluminium foil and low concentrations of a cryoprotectant. Cooling and warming rates of approximately 2000 and 3900 °Cminˇ1, respectively, were achieved as the thermal capacity of the foil-tray was significantly reduced compared to the aluminium sandwich device introduced by Day et al. [S.H. Day, D.A. Nicoll-Grifith, J.M. Silva, Cryopreservation of rat and human liver slices by rapid freezing, Cryobiology 38 (1999) 154-159]. Additionally, the two critical steps involved in the sandwich approach, i.e., clamping the plates and complete filling of the entire space between the plates with liquid, can be omitted using the foil tray. The viability of the slices was verified by measuring tetrazolium salt reduction capacity, cytosolic enzyme lact Kryoprezervace poskytuje možnost maximalizovat využití a dostupnost biologických materiálů, jejichž zdroje jsou omezené. Tato studie předkládá zlepšenou techniku současné kryoprezervace serie řezů jaterní tkáně s využitím přípravku z hliníkové folie při nízké koncentraci kryoprotektantu. Rychlost zchlazení a zahřívání přibližně 2000 a 3900 °C.min-1 je dosažena významným snížením tepelné kapacity hliníkové folie ve srovnání se sandwichovým zařízením, popsaným Dayem et al. [S.H. Day, D.A. Nicoll-Griffith, J.M. Silva, Cryopreservation of rat and human liver slices by rapid freezing, Cryobiology 38 (1999) 154-159]. Viabilita řezů byla ověřována měřením redukce tetrazoliové soli, uvolňování cytosolové laktátdehydrogenázy do media a metabolismu ethoxykumarinu. Cryopreservation oVers the potential to maximize the use and availability of biological materials that have a limited supply. This study demonstrates an enhanced technique for the parallel cryopreservation of a series of liver tissue slices using a tray modeled from aluminium foil and low concentrations of a cryoprotectant. Cooling and warming rates of approximately 2000 and 3900 °Cminˇ1, respectively, were achieved as the thermal capacity of the foil-tray was significantly reduced compared to the aluminium sandwich device introduced by Day et al. [S.H. Day, D.A. Nicoll-Grifith, J.M. Silva, Cryopreservation of rat and human liver slices by rapid freezing, Cryobiology 38 (1999) 154-159]. Additionally, the two critical steps involved in the sandwich approach, i.e., clamping the plates and complete filling of the entire space between the plates with liquid, can be omitted using the foil tray. The viability of the slices was verified by measuring tetrazolium salt reduction capacity, cytosolic enzyme lact
dcterms:title
A novel simplified ultra-rapid freezing technique for cryopreservation of tissue slices Nová zjednodušená technika ultrarychlého zmrazování pro kryoprezervaci tkáňových řezů A novel simplified ultra-rapid freezing technique for cryopreservation of tissue slices
skos:prefLabel
A novel simplified ultra-rapid freezing technique for cryopreservation of tissue slices Nová zjednodušená technika ultrarychlého zmrazování pro kryoprezervaci tkáňových řezů A novel simplified ultra-rapid freezing technique for cryopreservation of tissue slices
skos:notation
RIV/61989592:15110/06:00002714!RIV07-MSM-15110___
n3:strany
193-199
n3:aktivita
n4:Z n4:P
n3:aktivity
P(OC B17.20), Z(MSM6198959205)
n3:cisloPeriodika
2
n3:dodaniDat
n9:2007
n3:domaciTvurceVysledku
n13:5047633 n13:5628288 n13:6974899 n13:6087914 n13:1579843
n3:druhVysledku
n11:J
n3:duvernostUdaju
n8:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
463736
n3:idVysledku
RIV/61989592:15110/06:00002714
n3:jazykVysledku
n14:eng
n3:klicovaSlova
Cryopreservation; Precision-cut slices; Ultra-rapid freezing; Warming rate; Mouse liver
n3:klicoveSlovo
n6:Precision-cut%20slices n6:Cryopreservation n6:Ultra-rapid%20freezing n6:Mouse%20liver n6:Warming%20rate
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[771D980F8CE5]
n3:nazevZdroje
Cryobiology
n3:obor
n7:FB
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
6
n3:projekt
n18:OC%20B17.20
n3:rokUplatneniVysledku
n9:2006
n3:svazekPeriodika
52
n3:tvurceVysledku
Florschutz, Anthony V. Červenková, Kateřina Uherková, Lenka Rypka, Miroslav Veselý, Jaroslav Poczatková, Hana
n3:zamer
n17:MSM6198959205
s:issn
0011-2240
s:numberOfPages
7
n19:organizacniJednotka
15110