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Statements

Subject Item
n2:RIV%2F61989592%3A15110%2F05%3A00001984%21RIV07-MSM-15110___
rdf:type
n13:Vysledek skos:Concept
dcterms:description
Byl sledován vliv neesterifikovaných mastných kyselin (NEFA) a hormonu dehydroepiandrosteronu na hladiny mRNA isoforem δ a  proteinkinázy C (PKC) a mRNA liver fatty acid binding proteinu (L-FABP) v lidských hepatomových buňkách HepG2. Buňky byly po dobu experimentu udržovány v mediu s nízkým obsahem séra a albuminu. Zvolená koncentrace mastných kyselin byla 100 µM a časové intervaly 4 a 20 hodin. Za těchto podmínek nasycené (palmitová, stearová) a mononenasycené (olejová) kyseliny poměrně selektivně stimulovaly mRNA PKC . Neočekávaně jsme zjistili, že tyto mastné kyseliny také snižují hladinu mRNA L-FABP. DHEA ve farmakologických dávkách (100 μM) vyvolává znatelné zvýšení hladin mRNA PKC-δ a -ε. I když v tomto příspěvku nebyly zkoumány molekulární mechanismy na pozadí zjištěných změn, naše výsledky zdůrazňují, že modulace hladin mRNA, indukované NEFA, představují další mechanismus, kterým mohou NEFA v prostředí ovlivňovat metabolickou homeo The effects of non-esterified fatty acids (NEFA) and hormone dehydroepiandrosterone (DHEA) on the levels of mRNAs of protein kinase C (PKC) -δ and -ε isoforms and those of liver fatty acid binding protein (L-FABP) were investigated in the human hepatoma HepG2 cell line. The cells were kept in low-serum, low-albumin medium during experiments. Low FA levels (100 μM) and time intervals of 4 h and 20 h were used. In these conditions, the saturated (palmitic, stearic) and monounsaturated (oleic) acids rather selectively stimulated PKC-ε mRNA levels. Unexpectedly, we found that these acids also suppressed liver fatty-acid binding protein (L-FABP) mRNA levels. DHEA in pharmacological doses (100 μM) produced a significant increase in PKC-δ and -ε mRNA levels. Although molecular mechanisms underlying the identified changes have not been investigated in this paper, our findings emphasize that NEFA-induced modulation of mRNA levels of key signalling The effects of non-esterified fatty acids (NEFA) and hormone dehydroepiandrosterone (DHEA) on the levels of mRNAs of protein kinase C (PKC) -δ and -ε isoforms and those of liver fatty acid binding protein (L-FABP) were investigated in the human hepatoma HepG2 cell line. The cells were kept in low-serum, low-albumin medium during experiments. Low FA levels (100 μM) and time intervals of 4 h and 20 h were used. In these conditions, the saturated (palmitic, stearic) and monounsaturated (oleic) acids rather selectively stimulated PKC-ε mRNA levels. Unexpectedly, we found that these acids also suppressed liver fatty-acid binding protein (L-FABP) mRNA levels. DHEA in pharmacological doses (100 μM) produced a significant increase in PKC-δ and -ε mRNA levels. Although molecular mechanisms underlying the identified changes have not been investigated in this paper, our findings emphasize that NEFA-induced modulation of mRNA levels of key signalling
dcterms:title
Změny v hladinách mRNA intracelulárních regulátorů mastných kyselin působením neesterifikovaných mastných kyselin a dehydroepiandrosteronu na lidské hepatomové buňky HepG2 Changes in mRNA levels of intracellular fatty acid metabolism regulators in human hepatoma HepG2 cells following their treatment with non-esterified fatty acids and dehydroepiandrosterone Changes in mRNA levels of intracellular fatty acid metabolism regulators in human hepatoma HepG2 cells following their treatment with non-esterified fatty acids and dehydroepiandrosterone
skos:prefLabel
Změny v hladinách mRNA intracelulárních regulátorů mastných kyselin působením neesterifikovaných mastných kyselin a dehydroepiandrosteronu na lidské hepatomové buňky HepG2 Changes in mRNA levels of intracellular fatty acid metabolism regulators in human hepatoma HepG2 cells following their treatment with non-esterified fatty acids and dehydroepiandrosterone Changes in mRNA levels of intracellular fatty acid metabolism regulators in human hepatoma HepG2 cells following their treatment with non-esterified fatty acids and dehydroepiandrosterone
skos:notation
RIV/61989592:15110/05:00001984!RIV07-MSM-15110___
n3:strany
251-256
n3:aktivita
n9:P n9:Z
n3:aktivity
P(1P05OC063), P(1P05OC064), P(OC B17.20), Z(MSM6198959205)
n3:cisloPeriodika
2
n3:dodaniDat
n14:2007
n3:domaciTvurceVysledku
n4:5047633 n4:6087914 n4:6974899 n4:5628288 n4:1579843
n3:druhVysledku
n6:J
n3:duvernostUdaju
n12:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
514913
n3:idVysledku
RIV/61989592:15110/05:00001984
n3:jazykVysledku
n19:eng
n3:klicovaSlova
Fatty acids; Protein kinase C (PKC); Liver fatty acid binding protein (L-FABP); Insulin resistance; HepG2 cells
n3:klicoveSlovo
n8:Liver%20fatty%20acid%20binding%20protein%20%28L-FABP%29 n8:Insulin%20resistance n8:Protein%20kinase%20C%20%28PKC%29 n8:Fatty%20acids n8:HepG2%20cells
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[3F66F2ED46DC]
n3:nazevZdroje
Biomedical Papers
n3:obor
n17:FB
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
6
n3:projekt
n15:1P05OC063 n15:1P05OC064 n15:OC%20B17.20
n3:rokUplatneniVysledku
n14:2005
n3:svazekPeriodika
149
n3:tvurceVysledku
Uherková, Lenka Červenková, Kateřina Veselý, Jaroslav Rypka, Miroslav Poczatková, Hana Bogdanová, Kateřina
n3:zamer
n11:MSM6198959205
s:issn
1213-8118
s:numberOfPages
6
n18:organizacniJednotka
15110