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Statements

Subject Item
n2:RIV%2F61989592%3A15110%2F05%3A00001951%21RIV06-MSM-15110___
rdf:type
n3:Vysledek skos:Concept
dcterms:description
Arange of benzylaminopurines naturally occur in plants and exhibit high biological activity. Others have been synthesized, such as 6-(2-hydroxy-3-methoxybenzylamino)purine riboside (2OH3MeOBAPR), which has shown interesting anti-cancer activity under in vitro conditions. In orderto study the biological activity of this interesting compound in more detail, a rapid and highly efficient method for its purification from complexsamples (e.g. blood and plant extracts) is needed. Therefore, we prepared monoclonal antibodies against 2OH3MeOBAPR. The antibody hadundetectable cross-reactivity with all natural isoprenoid cytokinins, but relatively high cross-reactivity with aromatic cytokinins as well as somesynthetic di- and tri-substituted 6-benzylaminopurines and the corresponding ribosides. The antibody also showed strong responses and specificityin enzyme-linked immunoassays (ELISAs). In addition, it was used to prepare, for the first time, an immunoaffinity sorbent with high specificityand capacity for aro Přirozené benzylaminopuriny přitomné v rostlinách mají vysokou bilogickou aktivitu. Další byly syntetizované jak npř. 6-(2-hydroxy-3-methoxybenzylamino)purine riboside (2OH3MeOBAPR), které vykazují vysokou protinádorovou aktivitu v in vitro podmínkách. Arange of benzylaminopurines naturally occur in plants and exhibit high biological activity. Others have been synthesized, such as 6-(2-hydroxy-3-methoxybenzylamino)purine riboside (2OH3MeOBAPR), which has shown interesting anti-cancer activity under in vitro conditions. In orderto study the biological activity of this interesting compound in more detail, a rapid and highly efficient method for its purification from complexsamples (e.g. blood and plant extracts) is needed. Therefore, we prepared monoclonal antibodies against 2OH3MeOBAPR. The antibody hadundetectable cross-reactivity with all natural isoprenoid cytokinins, but relatively high cross-reactivity with aromatic cytokinins as well as somesynthetic di- and tri-substituted 6-benzylaminopurines and the corresponding ribosides. The antibody also showed strong responses and specificityin enzyme-linked immunoassays (ELISAs). In addition, it was used to prepare, for the first time, an immunoaffinity sorbent with high specificityand capacity for aro
dcterms:title
Batch immunoextraction method for an effective purification of aromatic cytokinins. Série imunoextrakčních metod na efektivní purifikaci aromatických cytokininů. Batch immunoextraction method for an effective purification of aromatic cytokinins.
skos:prefLabel
Batch immunoextraction method for an effective purification of aromatic cytokinins. Batch immunoextraction method for an effective purification of aromatic cytokinins. Série imunoextrakčních metod na efektivní purifikaci aromatických cytokininů.
skos:notation
RIV/61989592:15110/05:00001951!RIV06-MSM-15110___
n5:strany
116-125
n5:aktivita
n14:Z
n5:aktivity
Z(MSM6198959216)
n5:cisloPeriodika
1
n5:dodaniDat
n12:2006
n5:domaciTvurceVysledku
n6:4952340 n6:4712048 n6:9896821 n6:1263684 n6:7608543 n6:4012240 n6:5991803 n6:6085210
n5:druhVysledku
n16:J
n5:duvernostUdaju
n13:S
n5:entitaPredkladatele
n18:predkladatel
n5:idSjednocenehoVysledku
513575
n5:idVysledku
RIV/61989592:15110/05:00001951
n5:jazykVysledku
n8:eng
n5:klicovaSlova
antibody; batch immunoextraction; 6-benzylaminopurine
n5:klicoveSlovo
n10:batch%20immunoextraction n10:antibody n10:6-benzylaminopurine
n5:kodStatuVydavatele
NL - Nizozemsko
n5:kontrolniKodProRIV
[C31E3776BAE1]
n5:nazevZdroje
Journal of Chromatography A: Symposium Volumes
n5:obor
n15:FD
n5:pocetDomacichTvurcuVysledku
8
n5:pocetTvurcuVysledku
9
n5:rokUplatneniVysledku
n12:2005
n5:svazekPeriodika
1100
n5:tvurceVysledku
Popa, Igor Hauserová, Eva Strnad, Miroslav Hajdúch, Marián Vydra, David Swaczynová, Jana Doležal, Karel Lenobel, René Fuksová, Květoslava
n5:zamer
n11:MSM6198959216
s:issn
0021-9673
s:numberOfPages
10
n17:organizacniJednotka
15110