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Statements

Subject Item
n2:RIV%2F61989592%3A15110%2F04%3A00002001%21RIV06-MSM-15110___
rdf:type
skos:Concept n15:Vysledek
dcterms:description
V této práci jsme studovali, zda jsou mikrotubuly a %22heat shock%22 protein 90 (Hsp90) v buňkách HL-60 diferencovaných dimethylsulfoxidem zapojeny do oxidativního vzplanutí aktivovaného 12-O-myristoylforbol-13-acetátem (PMA) a peptidem N-formyl-Met-Leu-Phe (fMLP). Výsledky ukázaly, že látky interagující s mikrotubuly, paklitaxel (1-5 #M), kolchicin (1-100 #M), nokodazol (1-20 #M) a vinkristin (1-50 #M), neovlivňují oxidativní vzplanutí aktivované PMA ani fMLP. Naproti tomu radicikol, inhibitor proteinu Hsp90, inhiboval časově a koncentračně závislým způsobem oxidativní vzplanutí aktivované fMLP, přičemž hodnota IC50 měla při preinkubaci 30 min hodnotu 16,5 # 3,5 #M. Z výsledků usuzujeme, že oxidativní vzplanutí v diferencovaných buňkách HL-60 aktivované PMA i fMLP je nezávislé na mikrotubulech a že vzplanutí vyvolané fMLP vyžaduje aktivitu Hsp90. In this study we examined whether microtubules and heat shock protein 90 (Hsp90) are involved in phorbol myristate acetate (PMA) and N-formyl-Met-Leu-Phe (fMLP)-induced oxidative burst in DMSO-differentiated HL-60 cells. Our results showed that microtubule interfering agents, paclitaxel (1-5 microM), colchicine (1-100 microM), nocodazole (1-20 microM), and vincristine (1-50 microM), did not affect either PMA or fMLP-induced oxidative burst. In contrast, radicicol, an inhibitor of Hsp90, inhibited fMLP-induced oxidative burst in time and concentration-dependent manner where IC50 value for 30 min pre-incubation was 16.5 +/- 3.5 microM radicicol. We conclude that both PMA and fMLP-induced oxidative burst in DMSO-differentiated HL-60 cells is microtubule-independent while the latter requires Hsp90 activity. In this study we examined whether microtubules and heat shock protein 90 (Hsp90) are involved in phorbol myristate acetate (PMA) and N-formyl-Met-Leu-Phe (fMLP)-induced oxidative burst in DMSO-differentiated HL-60 cells. Our results showed that microtubule interfering agents, paclitaxel (1-5 microM), colchicine (1-100 microM), nocodazole (1-20 microM), and vincristine (1-50 microM), did not affect either PMA or fMLP-induced oxidative burst. In contrast, radicicol, an inhibitor of Hsp90, inhibited fMLP-induced oxidative burst in time and concentration-dependent manner where IC50 value for 30 min pre-incubation was 16.5 +/- 3.5 microM radicicol. We conclude that both PMA and fMLP-induced oxidative burst in DMSO-differentiated HL-60 cells is microtubule-independent while the latter requires Hsp90 activity.
dcterms:title
N-formyl-Met-Leu-Phe-induced oxidative burst in DMSO-differentiated HL-60 cells requires active Hsp90, but not intact microtubules. N-formyl-Met-Leu-Phe-induced oxidative burst in DMSO-differentiated HL-60 cells requires active Hsp90, but not intact microtubules. Oxidativní vzplanutí aktivované peptidem N-formyl-Met-Leu-Phe vyžaduje v buňkách HL-60 diferencovaných dimethylsulfoxidem aktivní Hsp90, ale nevyžaduje neporušené mikrotubuly
skos:prefLabel
N-formyl-Met-Leu-Phe-induced oxidative burst in DMSO-differentiated HL-60 cells requires active Hsp90, but not intact microtubules. N-formyl-Met-Leu-Phe-induced oxidative burst in DMSO-differentiated HL-60 cells requires active Hsp90, but not intact microtubules. Oxidativní vzplanutí aktivované peptidem N-formyl-Met-Leu-Phe vyžaduje v buňkách HL-60 diferencovaných dimethylsulfoxidem aktivní Hsp90, ale nevyžaduje neporušené mikrotubuly
skos:notation
RIV/61989592:15110/04:00002001!RIV06-MSM-15110___
n4:strany
141-144
n4:aktivita
n18:Z
n4:aktivity
Z(MSM 151100003)
n4:cisloPeriodika
2
n4:dodaniDat
n7:2006
n4:domaciTvurceVysledku
n9:1810596 n9:1030957
n4:druhVysledku
n6:J
n4:duvernostUdaju
n5:S
n4:entitaPredkladatele
n17:predkladatel
n4:idSjednocenehoVysledku
576255
n4:idVysledku
RIV/61989592:15110/04:00002001
n4:jazykVysledku
n13:eng
n4:klicovaSlova
Radicicol; Hsp90; Microtubules; Oxidative burst; HL-60
n4:klicoveSlovo
n8:HL-60 n8:Oxidative%20burst n8:Hsp90 n8:Microtubules n8:Radicicol
n4:kodStatuVydavatele
CZ - Česká republika
n4:kontrolniKodProRIV
[F54B72B9E76C]
n4:nazevZdroje
Biomedical Papers
n4:obor
n14:CE
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
2
n4:rokUplatneniVysledku
n7:2004
n4:svazekPeriodika
148
n4:tvurceVysledku
Modrianský, Martin Vrba, Jiří
n4:zamer
n12:MSM%20151100003
s:issn
1213-8118
s:numberOfPages
4
n16:organizacniJednotka
15110