This HTML5 document contains 55 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n15http://localhost/temp/predkladatel/
n10http://linked.opendata.cz/resource/domain/vavai/projekt/
n7http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n14http://linked.opendata.cz/ontology/domain/vavai/
n9http://linked.opendata.cz/resource/domain/vavai/zamer/
shttp://schema.org/
skoshttp://www.w3.org/2004/02/skos/core#
n3http://linked.opendata.cz/ontology/domain/vavai/riv/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n18http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F61989592%3A15110%2F01%3A00006958%21RIV09-MSM-15110___/
n4http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n5http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n19http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n16http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n17http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n12http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n8http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F61989592%3A15110%2F01%3A00006958%21RIV09-MSM-15110___
rdf:type
n14:Vysledek skos:Concept
dcterms:description
Cryopreservation of porcine hepatocytes would ensure the accessibility of cells for laboratory use, permit the standardisation of experiments and save animals? lives. Therefore, in this study, we sought the optimal procedure for cryopreservation of porcine hepatocytes for both laboratory and clinical purposes. Hepatocytes were isolated from the liver lobe of a mini-pig by two-step collagenase perfusion. The cells were frozen with 20% foetal calf serum and 15% DMSO in two different media in four different concentrations ranging from 1 x 106 cells/ml to 5 x 106 cells/ml. 1.8 ml cryotubes and 120 ml Baxter bags were used for this purpose. Cells were cryopreserved either in a controlled freezer Sylab or step by step in a styrofoam box and stored at -196 ºC. The quality of fresh and cryopreserved hepatocytes were assessed by trypan blue exclusion test and by the evaluation of cytochrome P450 isoenzymes and glutathione-S-transferase activities; primary cultures were evaluated morphologically and by Cryopreservation of porcine hepatocytes would ensure the accessibility of cells for laboratory use, permit the standardisation of experiments and save animals? lives. Therefore, in this study, we sought the optimal procedure for cryopreservation of porcine hepatocytes for both laboratory and clinical purposes. Hepatocytes were isolated from the liver lobe of a mini-pig by two-step collagenase perfusion. The cells were frozen with 20% foetal calf serum and 15% DMSO in two different media in four different concentrations ranging from 1 x 106 cells/ml to 5 x 106 cells/ml. 1.8 ml cryotubes and 120 ml Baxter bags were used for this purpose. Cells were cryopreserved either in a controlled freezer Sylab or step by step in a styrofoam box and stored at -196 ºC. The quality of fresh and cryopreserved hepatocytes were assessed by trypan blue exclusion test and by the evaluation of cytochrome P450 isoenzymes and glutathione-S-transferase activities; primary cultures were evaluated morphologically and by Kryoprezervace prasečích hepatocytů by zabezpečila dostupnost buněk pro laboratorní užití, dovolila standardizaci experimentů a ušetřila životy zvířat. V této práci jsme proto hledali nejlepší postup kryoprezervace prasečích hepatocytů jak pro laboratorní, tak pro klinické účely. Hepatocyty byly izolovány z jaterního laloku experimentálního miniaturního prasete pomocí dvoustupňové perfúze kolagenázou. Buňky byly poté zamraženy ve 20% fetálním telecím séru a 15% DMSO ve 2 různých médiích ve 4 koncentracích od 1 x 106 buněk/ml do 5 x 106 buněk/ml. Použity byly kryotuby o objemu 1.8 ml a vaky Baxter o objemu 120 ml. Buňky byly zamraženy buď řízeným mražením v přístroji Sylab, nebo postupným ochlazováním v polystyrénových boxech a poté uchovávány při -196 ºC. Kvalita čerstvých a kryoprezervovaných hepatocytů byla hodnocena v testu s trypanovou modří a měřením aktivity izoenzymů cytochromu P450 a glutathion-S-transferázy; primární kultury byly posuzovány morfologicky a testem MTT. Kryoprezervované h
dcterms:title
Optimizace kryoprezervace prasečích hepatocytů srovnáním životnosti a enzymatické aktivity čerstvých a kryoprezervovaných buněk Optimisation of porcine hepatocyte cryopreservation by comparison of viability and enzymatic activity of fresh and cryopreserved cells Optimisation of porcine hepatocyte cryopreservation by comparison of viability and enzymatic activity of fresh and cryopreserved cells
skos:prefLabel
Optimisation of porcine hepatocyte cryopreservation by comparison of viability and enzymatic activity of fresh and cryopreserved cells Optimisation of porcine hepatocyte cryopreservation by comparison of viability and enzymatic activity of fresh and cryopreserved cells Optimizace kryoprezervace prasečích hepatocytů srovnáním životnosti a enzymatické aktivity čerstvých a kryoprezervovaných buněk
skos:notation
RIV/61989592:15110/01:00006958!RIV09-MSM-15110___
n3:aktivita
n19:P n19:Z
n3:aktivity
P(GA311/98/0648), Z(MSM 141100003), Z(MSM 151100003), Z(MZE-M03-99-01)
n3:cisloPeriodika
2
n3:dodaniDat
n8:2009
n3:domaciTvurceVysledku
n7:8069239 n7:2514559 n7:8738394
n3:druhVysledku
n17:J
n3:duvernostUdaju
n5:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
690305
n3:idVysledku
RIV/61989592:15110/01:00006958
n3:jazykVysledku
n16:eng
n3:klicovaSlova
Freezing conditions; MTT test; cytochrom P450; primary cultures; porcine hepatocytes
n3:klicoveSlovo
n4:porcine%20hepatocytes n4:MTT%20test n4:Freezing%20conditions n4:cytochrom%20P450 n4:primary%20cultures
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[429A36C4CC26]
n3:nazevZdroje
Acta Veterinaria Brno
n3:obor
n12:CE
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
8
n3:projekt
n10:GA311%2F98%2F0648
n3:rokUplatneniVysledku
n8:2001
n3:svazekPeriodika
70
n3:tvurceVysledku
Dvořák, Zdeněk Dítě, Petr Lata, Jan Ulrichová, Jitka Smržová, Jana Machala, Miroslav Bláha, Luděk Šimánek, Vilím
n3:zamer
n9:MSM%20141100003 n9:MSM%20151100003 n9:MZE-M03-99-01
s:issn
0001-7213
s:numberOfPages
7
n15:organizacniJednotka
15110