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Statements

Subject Item
n2:RIV%2F61988987%3A17310%2F13%3AA14018DZ%21RIV14-MSM-17310___
rdf:type
n5:Vysledek skos:Concept
dcterms:description
The existence of two cryptic species within strains of the wood-decaying fungus Fomes fomentarius was revealed recently based on the internal transcribed spacer (ITS) sequence variability. In this study for the first time the sequences of another molecular markers, partial translation elongation factor 1-? (efa) region and partial 25S large subunit ribosomal RNA gene were obtained and used to evaluate genetic variability of F. fomentarius. Congruent phylogeny was observed for all three markers used confirming the presence of two cryptic species within F. fomentarius. Surprisingly, ITS sequence variability within F. fomentarius was significantly lower compared to the variability of efa sequences (0.023 versus 0.036 nucleotide substitutions per site) questioning the discriminatory power of ITS sequences for fungal species identification. The existence of two cryptic species within strains of the wood-decaying fungus Fomes fomentarius was revealed recently based on the internal transcribed spacer (ITS) sequence variability. In this study for the first time the sequences of another molecular markers, partial translation elongation factor 1-? (efa) region and partial 25S large subunit ribosomal RNA gene were obtained and used to evaluate genetic variability of F. fomentarius. Congruent phylogeny was observed for all three markers used confirming the presence of two cryptic species within F. fomentarius. Surprisingly, ITS sequence variability within F. fomentarius was significantly lower compared to the variability of efa sequences (0.023 versus 0.036 nucleotide substitutions per site) questioning the discriminatory power of ITS sequences for fungal species identification.
dcterms:title
Genetic variability in Fomes fomentarius reconfirmed by translation elongation factor 1-? DNA sequences and 25S LSU rRNA sequences Genetic variability in Fomes fomentarius reconfirmed by translation elongation factor 1-? DNA sequences and 25S LSU rRNA sequences
skos:prefLabel
Genetic variability in Fomes fomentarius reconfirmed by translation elongation factor 1-? DNA sequences and 25S LSU rRNA sequences Genetic variability in Fomes fomentarius reconfirmed by translation elongation factor 1-? DNA sequences and 25S LSU rRNA sequences
skos:notation
RIV/61988987:17310/13:A14018DZ!RIV14-MSM-17310___
n5:predkladatel
n7:orjk%3A17310
n3:aktivita
n8:V
n3:aktivity
V
n3:cisloPeriodika
5
n3:dodaniDat
n9:2014
n3:domaciTvurceVysledku
n18:4599942
n3:druhVysledku
n13:J
n3:duvernostUdaju
n6:S
n3:entitaPredkladatele
n12:predkladatel
n3:idSjednocenehoVysledku
76398
n3:idVysledku
RIV/61988987:17310/13:A14018DZ
n3:jazykVysledku
n14:eng
n3:klicovaSlova
Polyporaceae; cryptic species; Fomes fomentarius; molecular phylogeny; variability
n3:klicoveSlovo
n4:Polyporaceae n4:molecular%20phylogeny n4:cryptic%20species n4:Fomes%20fomentarius n4:variability
n3:kodStatuVydavatele
SK - Slovenská republika
n3:kontrolniKodProRIV
[AF9477A5F7BA]
n3:nazevZdroje
BIOLOGIA
n3:obor
n17:EH
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
4
n3:rokUplatneniVysledku
n9:2013
n3:svazekPeriodika
68
n3:tvurceVysledku
Judova, Jana Gaperova, Svetlana Pristas, Peter Gáper, Ján
n3:wos
000323739200006
s:issn
0006-3088
s:numberOfPages
5
n15:organizacniJednotka
17310