This HTML5 document contains 50 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n15http://localhost/temp/predkladatel/
n19http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n6http://linked.opendata.cz/resource/domain/vavai/projekt/
n13http://linked.opendata.cz/ontology/domain/vavai/
n10http://linked.opendata.cz/resource/domain/vavai/zamer/
shttp://schema.org/
skoshttp://www.w3.org/2004/02/skos/core#
n3http://linked.opendata.cz/ontology/domain/vavai/riv/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n16http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F61988987%3A17310%2F04%3AA1000V4D%21RIV10-MSM-17310___/
n7http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n14http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n11http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n4http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n18http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n12http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n17http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F61988987%3A17310%2F04%3AA1000V4D%21RIV10-MSM-17310___
rdf:type
n13:Vysledek skos:Concept
dcterms:description
Using a new competition assay, we investigated the effect of DNA negative supercoiling on the DNA sequence-specific binding ( SSDB) of human wild- type ( wt) p53 protein. We found that supercoiled ( sc) pBluescript DNAs with different inserted p53 target sequences were stronger competitors than a mixture of scDNA pBluescript with the given 20- mer target oligodeoxynucleotide. ScDNAs were always better competitors than their linearized or relaxed forms. Two DNAs with extruded cruciforms within the target sequence were the best competitors; removal of the cruciforms resulted in a decrease of competitor strength. In contrast to the full- length wt p53, the deletion mutant p53CDelta30 and the p53 core domain ( 93 - 312 aa) showed no enhancement of p53 SSDB to scDNA, suggesting that, in addition to the p53 core domain, the C- terminal was involved in this binding. Using a new competition assay, we investigated the effect of DNA negative supercoiling on the DNA sequence-specific binding ( SSDB) of human wild- type ( wt) p53 protein. We found that supercoiled ( sc) pBluescript DNAs with different inserted p53 target sequences were stronger competitors than a mixture of scDNA pBluescript with the given 20- mer target oligodeoxynucleotide. ScDNAs were always better competitors than their linearized or relaxed forms. Two DNAs with extruded cruciforms within the target sequence were the best competitors; removal of the cruciforms resulted in a decrease of competitor strength. In contrast to the full- length wt p53, the deletion mutant p53CDelta30 and the p53 core domain ( 93 - 312 aa) showed no enhancement of p53 SSDB to scDNA, suggesting that, in addition to the p53 core domain, the C- terminal was involved in this binding.
dcterms:title
Enhancement of p53 sequence-specific binding by DNA supercoiling Enhancement of p53 sequence-specific binding by DNA supercoiling
skos:prefLabel
Enhancement of p53 sequence-specific binding by DNA supercoiling Enhancement of p53 sequence-specific binding by DNA supercoiling
skos:notation
RIV/61988987:17310/04:A1000V4D!RIV10-MSM-17310___
n3:aktivita
n11:P n11:V n11:Z
n3:aktivity
P(GA301/00/D001), P(IAA4004110), P(IAB5004203), P(OC D21.002), V, Z(AV0Z5004920)
n3:cisloPeriodika
12
n3:dodaniDat
n17:2010
n3:domaciTvurceVysledku
n19:3787125
n3:druhVysledku
n12:J
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
562906
n3:idVysledku
RIV/61988987:17310/04:A1000V4D
n3:jazykVysledku
n4:eng
n3:klicovaSlova
competition assay; p53; DNA sequence-specific binding; supercoiled DNA; cruciform extrusion
n3:klicoveSlovo
n7:p53 n7:cruciform%20extrusion n7:supercoiled%20DNA n7:DNA%20sequence-specific%20binding n7:competition%20assay
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[142DB35D9AFD]
n3:nazevZdroje
ONCOGENE
n3:obor
n18:EB
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
6
n3:projekt
n6:GA301%2F00%2FD001 n6:OC%20D21.002 n6:IAA4004110 n6:IAB5004203
n3:rokUplatneniVysledku
n17:2004
n3:svazekPeriodika
23
n3:tvurceVysledku
Karlovská, Lenka Brázdová, Marie Paleček, Emil Brázda, Václav Pečinka, Petr Jagelská, Eva
n3:zamer
n10:AV0Z5004920
s:issn
0950-9232
s:numberOfPages
8
n15:organizacniJednotka
17310