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Statements

Subject Item
n2:RIV%2F61389030%3A_____%2F14%3A00441341%21RIV15-AV0-61389030
rdf:type
n10:Vysledek skos:Concept
dcterms:description
As a second messenger, calcium is involved in auxin signaling, and previous studies demonstrated that Arabidopsis CAX1 (Ca2+/H+ exchanger) is involved in the auxin transduction pathway. This study was performed to investigate the possible role of auxin-binding proteins ABP1 and ABP4 in Ca2+/auxin-regulated growth in maize (Zea mays L.). We identified and cloned two AtCAX1 homologs in maize, ZCAX2 and ZCAX3. Using maize loss-of-function abp1 and abp4 mutants, the role of ABPs in Ca2+-dependent growth and in the Ca2+/auxin-regulated expression of the CAX genes was investigated in etiolated maize seedlings. Exogenous Ca2+ enhanced mesocotyl but not coleoptile growth in WT, abp1, abp4, and abp1/abp4 mutants, but the maximum stimulation was in abp4. As well, in the abp4 mutant, maximum accumulation of Ca2+ was observed when seedlings were exposed to exogenous Ca2+. In the mesocotyl of abp4 and double mutants, the expression of ZCAX3 was significantly reduced in the absence of exogenous Ca2+, whereas exogenous Ca2+ significantly up-regulated its expression in both mutants. This effect of Ca2+ was not observed in the coleoptile. In the absence of NAA, knockout of ABP4 led to significant drop of ZCAX3 expression in the mesocotyl, and exogenous auxin significantly inhibited expression of the ZCAX3 in WT, but not in abp mutants. This effect of auxin was not observed in the coleoptile. Our results indicate that in the absence of NAA, functional ABP4 is required for ZCAX3 expression and that ABP4 mediates the inhibitory effect of NAA on ZCAX3 expression. We provided evidence for a cross talk between ABP4, exogenous auxin, Ca2+, and ZCAX3 during growth of etiolated maize mesocotyl. As a second messenger, calcium is involved in auxin signaling, and previous studies demonstrated that Arabidopsis CAX1 (Ca2+/H+ exchanger) is involved in the auxin transduction pathway. This study was performed to investigate the possible role of auxin-binding proteins ABP1 and ABP4 in Ca2+/auxin-regulated growth in maize (Zea mays L.). We identified and cloned two AtCAX1 homologs in maize, ZCAX2 and ZCAX3. Using maize loss-of-function abp1 and abp4 mutants, the role of ABPs in Ca2+-dependent growth and in the Ca2+/auxin-regulated expression of the CAX genes was investigated in etiolated maize seedlings. Exogenous Ca2+ enhanced mesocotyl but not coleoptile growth in WT, abp1, abp4, and abp1/abp4 mutants, but the maximum stimulation was in abp4. As well, in the abp4 mutant, maximum accumulation of Ca2+ was observed when seedlings were exposed to exogenous Ca2+. In the mesocotyl of abp4 and double mutants, the expression of ZCAX3 was significantly reduced in the absence of exogenous Ca2+, whereas exogenous Ca2+ significantly up-regulated its expression in both mutants. This effect of Ca2+ was not observed in the coleoptile. In the absence of NAA, knockout of ABP4 led to significant drop of ZCAX3 expression in the mesocotyl, and exogenous auxin significantly inhibited expression of the ZCAX3 in WT, but not in abp mutants. This effect of auxin was not observed in the coleoptile. Our results indicate that in the absence of NAA, functional ABP4 is required for ZCAX3 expression and that ABP4 mediates the inhibitory effect of NAA on ZCAX3 expression. We provided evidence for a cross talk between ABP4, exogenous auxin, Ca2+, and ZCAX3 during growth of etiolated maize mesocotyl.
dcterms:title
AUXIN BINDING PROTEIN 4 is involved in the Ca2+/auxin-regulated expression of ZCAX3 gene in maize (Zea mays) AUXIN BINDING PROTEIN 4 is involved in the Ca2+/auxin-regulated expression of ZCAX3 gene in maize (Zea mays)
skos:prefLabel
AUXIN BINDING PROTEIN 4 is involved in the Ca2+/auxin-regulated expression of ZCAX3 gene in maize (Zea mays) AUXIN BINDING PROTEIN 4 is involved in the Ca2+/auxin-regulated expression of ZCAX3 gene in maize (Zea mays)
skos:notation
RIV/61389030:_____/14:00441341!RIV15-AV0-61389030
n3:aktivita
n15:P n15:I
n3:aktivity
I, P(1P05ME792), P(ED2.1.00/03.0058)
n3:cisloPeriodika
5
n3:dodaniDat
n4:2015
n3:domaciTvurceVysledku
n5:4387244 n5:8983798 n5:1152661
n3:druhVysledku
n16:J
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n6:predkladatel
n3:idSjednocenehoVysledku
4688
n3:idVysledku
RIV/61389030:_____/14:00441341
n3:jazykVysledku
n13:eng
n3:klicovaSlova
auxin-binding protein; ABP4; AtCAX1
n3:klicoveSlovo
n12:ABP4 n12:AtCAX1 n12:auxin-binding%20protein
n3:kodStatuVydavatele
CA - Kanada
n3:kontrolniKodProRIV
[59DE494745A8]
n3:nazevZdroje
Botany
n3:obor
n7:EF
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
4
n3:projekt
n17:1P05ME792 n17:ED2.1.00%2F03.0058
n3:rokUplatneniVysledku
n4:2014
n3:svazekPeriodika
92
n3:tvurceVysledku
Jurišić-Knežev, Dejana Fellner, Martin Milde, D. Bergougnoux, Véronique
n3:wos
000335963300003
s:issn
1916-2790
s:numberOfPages
8
n11:doi
10.1139/cjb-2013-0262