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Statements

Subject Item
n2:RIV%2F61389030%3A_____%2F14%3A00433901%21RIV15-GA0-61389030
rdf:type
n6:Vysledek skos:Concept
rdfs:seeAlso
http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=MEDLINE&DestLinkType=FullRecord&UT=25059295
dcterms:description
Many proteins are present in the nucleus; some are involved with its structural and functional organization, some with gene expression, and some with cell division. The plant nuclear proteome has not been well explored. Its characterization requires extraction methods which minimize both the artifactual alteration of the proteins and the extent of contamination with non-nuclear proteins. The conventional multi-step fractionation procedure is both laborious and prone to contamination. Here, we describe a single-step method based on flow sorting. The method allows the separation of G1, S and G2 phase nuclei and minimizes the risk of contamination by non-nuclear proteins. Preliminary results obtained using G1 phase cell nuclei from barley root tips indicate that flow sorting coupled with a protein/peptide separation and mass spectrometry will permit a comprehensive characterization of the plant nuclear proteome. Many proteins are present in the nucleus; some are involved with its structural and functional organization, some with gene expression, and some with cell division. The plant nuclear proteome has not been well explored. Its characterization requires extraction methods which minimize both the artifactual alteration of the proteins and the extent of contamination with non-nuclear proteins. The conventional multi-step fractionation procedure is both laborious and prone to contamination. Here, we describe a single-step method based on flow sorting. The method allows the separation of G1, S and G2 phase nuclei and minimizes the risk of contamination by non-nuclear proteins. Preliminary results obtained using G1 phase cell nuclei from barley root tips indicate that flow sorting coupled with a protein/peptide separation and mass spectrometry will permit a comprehensive characterization of the plant nuclear proteome.
dcterms:title
Proteomic analysis of barley cell nuclei purified by flow sorting Proteomic analysis of barley cell nuclei purified by flow sorting
skos:prefLabel
Proteomic analysis of barley cell nuclei purified by flow sorting Proteomic analysis of barley cell nuclei purified by flow sorting
skos:notation
RIV/61389030:_____/14:00433901!RIV15-GA0-61389030
n3:aktivita
n17:I n17:S n17:P
n3:aktivity
I, P(EE2.3.20.0165), P(GA14-28443S), P(GBP501/12/G090), P(LO1204), S
n3:cisloPeriodika
1-3
n3:dodaniDat
n7:2015
n3:domaciTvurceVysledku
n8:4748174 n8:9606599 n8:7901321 n8:1800477
n3:druhVysledku
n16:J
n3:duvernostUdaju
n4:S
n3:entitaPredkladatele
n14:predkladatel
n3:idSjednocenehoVysledku
40483
n3:idVysledku
RIV/61389030:_____/14:00433901
n3:jazykVysledku
n15:eng
n3:klicovaSlova
Cell cycle; Chromatin; Flow cytometry
n3:klicoveSlovo
n11:Flow%20cytometry n11:Cell%20cycle n11:Chromatin
n3:kodStatuVydavatele
CH - Švýcarská konfederace
n3:kontrolniKodProRIV
[C13B0676931B]
n3:nazevZdroje
Cytogenetics and Genome Research
n3:obor
n18:EB
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
8
n3:projekt
n9:LO1204 n9:GA14-28443S n9:EE2.3.20.0165 n9:GBP501%2F12%2FG090
n3:rokUplatneniVysledku
n7:2014
n3:svazekPeriodika
143
n3:tvurceVysledku
Chamrád, I. Jeřábková, Hana Uřinovská, J. Doležel, Jaroslav Lenobel, R. Vrána, Jan Petrovská, Beáta Šebela, M.
n3:wos
000342614100008
s:issn
1424-8581
s:numberOfPages
9
n19:doi
10.1159/000365311